DNA damage and apoptosis in the mussel Mytilus galloprovincialis

Mičić, Milena; Bihari, Nevenka; Jakšić, Željko; Müller, Wernér E.G.; Batel, Renato

doi:10.1016/s0141-1136(01)00112-x

Cited by 42 publications

(11 citation statements)

References 43 publications

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“…Less well understood -but of pivotal importance to the integrity of the genome -is the role of temperature-induced damage to DNA. Many previous studies have shown that DNA damage is induced by various environmental chemical stressors including genotoxic substances, heavy metals and organic contaminants (Mičić et al, 2002;Klobučar et al, 2008;Wepener et al, 2008). However, relatively little is known about the effects of thermal stress on the integrity of DNA.…”

Section: Introductionmentioning

confidence: 99%

The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

Yao

Somero

2012

Journal of Experimental Biology

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SUMMARYWe investigated the effects of acute heat stress and cold stress on cell viability, lysosome membrane stability, double-and singlestranded DNA breakage, and signaling mechanisms involved in cellular homeostasis and apoptosis in hemocytes of native and invasive mussels, Mytilus californianus and Mytilus galloprovincialis, respectively. Both heat stress (28, 32°C) and cold stress (2, 6°C) led to significant double-and single-stranded breaks in DNA. The type and extent of DNA damage were temperature and time dependent, as was caspase-3 activation, an indicator of apoptosis, which may occur in response to DNA damage. Hemocyte viability and lysosomal membrane stability decreased significantly under heat stress. Western blot analyses of hemocyte extracts with antibodies for proteins associated with cell signaling and stress responses [including members of the phospho-specific mitogen-activated protein kinase (MAPK) family c-JUN NH 2 -terminal kinase (JNK) and p38-MAPK, and apoptosis executor caspase-3] revealed that heat and cold stress induced a time-dependent activation of JNK, p38-MAPK and caspase-3 and that these signaling and stress responses differed between species. The thermal limits for activation of cell signaling processes linked to the repair of stress-induced damage may help determine cellular thermal tolerance limits. Our results show similarities in responses to cold and heat stress and suggest causal linkages between levels of DNA damage at both extremes of temperature and downstream regulatory responses, including induction of apoptosis. Compared with M. californianus, M. galloprovincialis might have a wider temperature tolerance due to a lower amount of single-and double-stranded DNA damage, faster signaling activation and transduction, and stronger repair ability against temperature stress.

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Section: Introductionmentioning

confidence: 99%

The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

Yao

Somero

2012

Journal of Experimental Biology

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“…Accordingly, the other forms of abnormal nuclei recorded in the present study such as lobed nuclei and kidney-shaped nuclei may represent different precursors of micronuclei or binuclei phenomena (Harabawy and Mosleh, 2014). In addition fragmented-apoptotic cells can be considered as one of the principal mechanisms in elimination of micronucleated damaged cells Mičić et al, 2002). Although the mechanisms responsible for NAs have not been fully explained, the formation of different NAs may represent a way by which the cell reduces any amplified genetic material from the nucleus (Shimizu et al, 1998).…”

Section: Blood Alteration Detection 331 Genotoxic Effects: Micronumentioning

confidence: 62%

Biochemical and Histopathological Changes in Nile Tilapia, Oreochromis niloticus at Lake of Edku

Mourad¹,

Tanekhy²,

Wassif³

et al. 2017

AJVS

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Key words:Biochemical; Histopathology; Oreochromis niloticus; Lake EdkuThe main objective of this study is to assess some biochemical and histopathological changes in Nile Tilapia, Oreochromis niloticus caught from Lake Edku. A decrease in serum total protein, albumin, total lipids, bactericidal activity, ALT and AST was observed in El-Maadyah and Edku drain regions compared to recovery group, while the levels of serum glucose and lysozyme activity were increased. The frequencies of micronucleus (MN) and nuclear abnormalities (NAs) in fish erythrocytes showed an increase of micronuclei, lobed nuclei and fragmented apoptotic nuclei while notched nuclei were decreased. Finally, the frequencies of morphologically altered erythrocytes (MAEs) of fish showed an increase in swelled cells, vacuolated cytoplasm; microcytes; echinocytes; teardrop like; sickle cells and nuclear retraction. On the other hand, the main prominent hepatic lesions were parasitic granuloma, encapsulated parasitic cysts by connective tissue, fatty degeneration, congestion and necrosis. The histopathological analysis of muscle sections of both polluted sites showed edema, splitting of muscle fibers, hyalinized muscle fibers, fatty tissue, necrosis and infiltration of lymphocytes. From the above, we can conclude that the contaminated environment of fish could cause detrimental effects on fish tissues and immune system causing great economic losses for the natural resources.

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“…The RGD peptides have been used in many human cell types as inhibitors of integrin-ligand interactions and are well known to induce apoptosis integrin-dependently or -independently, for example, by direct caspase activation (Aoshiba et al 1997, Buckley et al 1999. Cadmium induced apoptosis in oyster C. virginica haemocytes in a dose-dependent manner (Sokolova et al 2004) similar to vertebrate cells (de la Fuente et al 2002), and trin-butyltin also induced apoptotic processes in gills of the mussel Mytilus galloprovincialis (Mi<ić et al 2001(Mi<ić et al , 2002. Lacoste et al (2002) studied the induction and signaling pathway of apoptosis in oyster C. gigas haemocytes and suggested that noradrenaline can elicit apoptosis via the b-adrenoceptor-mediated activator of adenylate cyclase and c-adenosine monophosphate (AMP)-dependent protein kinase.…”

Section: Abstract: Branchial Lesions · Apoptosis · Tem · Ostrea Edulmentioning

confidence: 80%

Branchial lesions associated with abundant apoptotic cells in oysters Ostrea edulis of Galicia (NW Spain)

Silva

Villalba²,

Sunila³

2006

Dis. Aquat. Org.

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DNA damage and apoptosis in the mussel Mytilus galloprovincialis

Cited by 42 publications

References 43 publications

The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

Biochemical and Histopathological Changes in Nile Tilapia, Oreochromis niloticus at Lake of Edku

Branchial lesions associated with abundant apoptotic cells in oysters Ostrea edulis of Galicia (NW Spain)

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