DNA cross-triggered cascading self-amplification artificial biochemical circuit

Nie, Ji; Zhao, Mi; Xie, Wen Jun; Cai, Liangyuan; Zhou, Ying‐Lin; Zhang, Xin‐Xiang

doi:10.1039/c4sc03225j

Cited by 22 publications

(10 citation statements)

References 29 publications

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“…For example, two EATR cycles can cross-trigger one another to achieve simultaneous self-amplification for use towards a DNA-based artificial biochemical circuit. 24 Additionally, cascaded catalytic nucleic acids have been used to control reactivity, perform logic operations and assemble complex structures. 25…”

Section: Target Recyclingmentioning

confidence: 99%

Approaches towards molecular amplification for sensing

Goggins

Frost

2016

Analyst

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Diagnostic assays that rely on molecular interactions have come a long way; from initial reversible detection systems towards irreversible reaction indicator-based methods. More recently, the emergence of innovative molecular amplification methodologies has revolutionised sensing, allowing diagnostic assays to achieve ultra-low limits of detection. There have been a significant number of molecular amplification approaches developed over recent years to accommodate the wide variety of analytes that require sensitive detection. To celebrate this achievement, this comprehensive critical review has been compiled to give a broad overview of the many different approaches used to attain amplification in sensing with an aim to inspire the next generation of diagnostic assays looking to achieve the ultimate detection limit. This review has been created with the focus on how each conceptually unique molecular amplification methodology achieves amplification, not just its sensitivity, while highlighting any key processes. Excluded are any references that were not found to contain an obvious molecular amplifier or amplification component, or that did not use an appropriate signal readout that could be incorporated into a sensing application. Additionally, methodologies where amplification is achieved through advances in instrumentation are also excluded. Depending upon the type of approach employed, amplification strategies are divided into four categories: target, label, signal or receptor amplification. More recent, more complex protocols combine a number of approaches and are therefore categorised by which amplification component described within was considered as the biggest advancement. The advantages and disadvantages of each methodology are discussed along with any limits of detection, if stated in the original article. Any subsequent use of the methodology within sensing or any other application is also mentioned to draw attention to its practicality. The importance of amplification within sensing is wholly emphasised while perspectives on the future direction of the field are also shared.

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Section: Target Recyclingmentioning

confidence: 99%

Approaches towards molecular amplification for sensing

Goggins

Frost

2016

Analyst

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“…Firstly, long ssDNA obtain from dsDNA samples through the combined action of nicking enzyme and polymerase (Jia et al, 2010;Qian et al, 2012;Nie et al, 2015;Shi et al, 2016). Then, hairpin self-assembly trigger recycling happen in the presence of ssDNA (Yin et al, 2008;Wu et al, 2015;Zang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

An electrochemical biosensor for double-stranded Wnt7B gene detection based on enzymatic isothermal amplification

Chen

et al. 2016

Biosensors and Bioelectronics

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“…and microRNAs in cancer cells are being studied as early biomarkers for determination of cancers. 7–9 These markers can be used to distinguish cancer cells from healthy cells. Highly sensitive detection of microRNAs can allow effective differentiation and quantification of cancer cells.…”

Section: Introductionmentioning

confidence: 99%