DNA controllable peroxidase-like activity of Ti3C2 nanosheets for colorimetric detection of microcystin-LR

Guo, Jingfang; Wang, Guodong; Zou, Jing; Lei, Zhen

doi:10.1007/s00216-023-04745-0

Cited by 5 publications

(1 citation statement)

References 44 publications

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“…There are many reported studies on the POD/OD-like properties of MXene-based nanomaterials. 37,75–84 Similar to horseradish peroxidase (HRP), the MXene- based nanomaterials 37 could also catalyze the reaction of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue-colored reaction. The catalytic activity was dependent on temperature, pH, and H 2 O 2 concentration (Fig.…”

Section: Enzymatic Properties Of Mxene-based Nanozymesmentioning

confidence: 99%

MXene-based nanomaterials with enzyme-like properties for biomedical applications

et al. 2023

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Section: Enzymatic Properties Of Mxene-based Nanozymesmentioning

confidence: 99%

MXene-based nanomaterials with enzyme-like properties for biomedical applications

et al. 2023

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Dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase-tethered magnetic microspheres for colorimetric detection of microcystin-LR

Zhang

Xue

et al. 2023

Microchim Acta

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A novel dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase (HRP) was developed for colorimetric determination of MC-LR. This dual-amplification was accomplished by combining the nuclease activity of CRISPR-Cas12a with the redox activity of HRP. HRP linked to magnetic beads through an ssDNA (MB-ssDNA-HRP) was used to induce a color change of the 3,3′,5,5′-tetramethylbenzidine (TMB)-H2O2 chromogenic substrate solution. Specific binding of MC-LR with its aptamer initiated the release of a complementary DNA (cDNA), which was designed to activate the trans-cleavage activity of CRISPR-Cas12a. Upon activation, Cas12a cut the ssDNA linker in MB-ssDNA-HRP, causing a reduction of HRP on the magnetic beads. Consequently, the UV–Vis absorbance of the HRP-catalyzed reaction was decreased. The dual-signal amplification facilitated by CRISPR-Cas12a and HRP enabled the colorimetric detection of MC-LR in the range 0.01 to 50 ng·mL−1 with a limit of detection (LOD) of 4.53 pg·mL−1. The practicability of the developed colorimetric method was demonstrated by detecting different levels of MC-LR in spiked real water samples. The recoveries ranged from 86.2 to 118.5% and the relative standard deviation (RSD) was 8.4 to 17.6%. This work provides new inspiration for the construction of effective signal amplification platforms and demonstrates a simple and user-friendly colorimetric method for determination of trace MC-LR. Graphical Abstract

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CeO2 nanocages with tetra-enzyme mimetic activities for dual-channel ratiometric colorimetric detection of microcystins-LR

Wang,

Guo,

Zou

et al. 2024

Analytica Chimica Acta

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DNA controllable peroxidase-like activity of Ti3C2 nanosheets for colorimetric detection of microcystin-LR

Cited by 5 publications

References 44 publications

MXene-based nanomaterials with enzyme-like properties for biomedical applications

MXene-based nanomaterials with enzyme-like properties for biomedical applications

Dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase-tethered magnetic microspheres for colorimetric detection of microcystin-LR

CeO2 nanocages with tetra-enzyme mimetic activities for dual-channel ratiometric colorimetric detection of microcystins-LR

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