DNA content polymorphism and tissue culture regeneration in Caribbean pine

Berlyn, Graeme P.; Anoruo, Ambrose O.; Beck, Richard C.E.; Cheng, Junjun

doi:10.1139/b87-132

Cited by 23 publications

(13 citation statements)

References 22 publications

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“…However, the present data render a reconsideration of their results. There is a long list of plants purportedly showing infraspecific genome size variation (see Bennett 1985;Greilhuber 1988b;Price 1988b), sometimes in association with developmental events (e.g., Berlyn et al 1987;Cavallini et al 1989;Nagl et al 1979;Schaffner and Nagl 1979). One has the impression that the old concept of the fairly stable genome has been largely replaced in recent years by the idea of the plastic genome.…”

Section: Bgrc Accessions 3 Levels Telophases and Prophases Togethermentioning

confidence: 99%

Genome size variation in Pisum sativum

Greilhuber¹,

Ebert²

1994

Genome

173

113

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Pisum sativum L. is one of the plant species where infraspecific genome size variation, up to 1.29-fold between cultivars, has been reported. The present investigation deals with a Feulgen cytophotometric analysis of this phenomenon in 25 wild accessions, landraces, and cultivars of widely different geographic origin. Differences between accessions were maximally 1.054-fold in single experiments but proved to be nonreproducible upon repeated measurements. Seedlings of the same accession often differed significantly, up to 1.056-fold, but values from root and shoot tips in one individual were not significantly correlated, indicating the absence of true genome size variation between plants. Upon calibration against Allium cepa a 1C value of 4.42 pg is estimated for Pisum sativum. Altogether the data suggest that, contrary to the divergence in the literature data and recent reports on DNA content variation, the pea has a stable genome size.

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Section: Bgrc Accessions 3 Levels Telophases and Prophases Togethermentioning

confidence: 99%

Genome size variation in Pisum sativum

Greilhuber¹,

Ebert²

1994

Genome

173

113

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“…In this study, rooting in shoots not treated with auxins occurred at 24%, which did not significantly differ from those treated with 0.5p~M NAA (20%), l p~M NAA (48%), 100 I~M NAA (38%), 1 p~M NAA + 10 p~M IBA (15%), and 10 p~M IBA (18%). Rooting without auxin pretreatment is an advantage since auxins have been associated with loss of genetic stability of tissue cultured P. caribaea plantlets (Berlyn et al 1987). Addition of organic supplements such as 1.2 ~M glyphosate (37%) and 1% coconut water (38%) did not improve the rooting efficiency of P. caribaea.…”

Section: Rooting Of Adventitious Shootsmentioning

confidence: 98%

“…They used Schenk and Hildebrandt's medium supplemented with 22.2 ~M BA, which induced 33% of the embryos to produce adventitious buds. Berlyn et al (1987) produced adventitious buds from mature embryos of P. caribaea using half-strength Campbell and Durzan's medium with 13.3-22.2 ~M BA. Baxter et al (1989) obtained clonal plantlets of P. caribaea via axillary shoot activation in tissue culture wherein 52.5% of P. caribaea clones produced from 11 up to 1,000 explants from seven transfers per year.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Pinus caribaea Morelet

Halos

1993

Plant Cell Tiss Organ Cult

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Adventitious shoot formation was induced in excised mature embryos of Pinus caribaea using a modified Murashige and Skoog medium (MSM) supplemented with 6-benzyladenine. The highest frequency (96%) of adventitious bud production was observed when embryos were exposed to 8.9 ~M BA for one week prior to transfer to a growth regulator-free medium. Increased BA concentration and longer exposure to BA significantly reduced survival rates of explants. Dilution of the basal medium to 1/4x and 1/8x decreased shoot formation but 1/2x was just as effective as full-strength. Addition of auxins, glyphosate and coconut water to the rooting medium did not improve rooting success beyond that of spontaneous rooting. Sucrose at 1.5% significantly increased rooting of shoots. Plantlets were successfully transferred to the soil after preincubation in liquid medium.

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“…hondurensis), and a third on the Bahamas and Caicos Islands (P. caribaea var. bahamensis; Berlyn, Anoruo, Beck, & Cheng, 1987;Anoruo & Berlyn, 1993). The three varieties are therefore isolated, and interbreeding does not occur in nature.…”

Section: The Ecological Species Conceptmentioning

confidence: 99%

The Relevance of Species Concepts in Sustainable Forestry

Marshall

Berlyn

Oliver

2014

Journal of Sustainable Forestry

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Species concepts and the definition of a species are discussed in the context of sustainable forest management and the sustainability of biological systems in general. Sustainable forestry requires that the habitats created by silvicultural manipulation match the ecological niches of the species being managed, enabling them not only to grow but to reproduce themselves so they can persist on the landscape beyond the current rotation. For this to be possible, foresters must be able to identify species and understand how species are defined.

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DNA content polymorphism and tissue culture regeneration in Caribbean pine

Cited by 23 publications

References 22 publications

Genome size variation in Pisum sativum

Genome size variation in Pisum sativum

Micropropagation of Pinus caribaea Morelet

The Relevance of Species Concepts in Sustainable Forestry

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