DNA Content of Stichococcus bacillaris (Trebouxiophyceae, Chlorophyta) Nuclei Determined with Laser Scanning Cytometry

Abstract

SummaryThe unicellular green microalgal genus Stichococcus Nägeli propagates by binary fission. In the present study, we measured the DNA content of Stichococcus bacillaris Nägeli in propidium iodide stained nuclei using laser scanning cytometry (LSC). Using the nuclei of Ulva compressa Linnaeus and Saccharomyces cerevisiae Hansen as references, the nuclear genome size of S. bacillaris was calculated as 80 Mbp. This value is approximately 4 times larger than that of Nannochloris bacillaris (20.3 Mbp), another member of the Trebouxiophyceae that propagates by binary fission.

Evaluating the Cytometric Detection and Enumeration of the Wine Bacterium, Oenococcus oeni

“…However, there are several bacteria that also most commonly exist as doublets or chains that can impact the method used for enumeration (23)(24)(25)(26). Additionally, it is critical to understand cellular morphology that may impact the enumeration method, such as binary fission or species-specific physiological changes (27,28).…”