DNA Content Differences Between Male and Female Chicken (Gallus gallus domesticus) Nuclei and Z and W Chromosomes Resolved by Image Cytometry

Mendonça, Maria Andréia Corrěa; Carvalho, Carlos Roberto

doi:10.1369/jhc.2009.954727

Cited by 25 publications

(24 citation statements)

References 37 publications

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“…Using trout blood as the internal standard, the haploid female chicken genome size (GS) determined by flow cytometry was 1.05 Gb (1.07 pg) as previously reported for female chicken [59]. We preferred rainbow trout nuclei as the internal standard in the assessment of the Artemia GS because their fluorescence values did not overlap with those of Artemia , as was the case with fluorescence values obtained from chicken nuclei.…”

Section: Resultsmentioning

confidence: 96%

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A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

et al. 2013

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We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp Artemia franciscana. Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2n = 42), covering 1,041 and 1,313 cM respectively. Fifteen putatively homologous linkage groups, including the sex linkage groups, were identified between the female and male linkage map. Eight sex-linked AFLP marker alleles were inherited from the female parent, supporting the hypothesis of a WZ–ZZ sex-determining system. The haploid Artemia genome size was estimated to 0.93 Gb by flow cytometry. The produced Artemia linkage maps provide the basis for further fine mapping and exploring of the sex-determining region and are a possible marker resource for mapping genomic loci underlying phenotypic differences among Artemia species.

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Section: Resultsmentioning

confidence: 96%

“…The haploid genome size (GS) of Artemia was assessed against the rainbow trout (haploid GS 2.4–3.0 pg or 2.35−2.93 Gb [58]) and the chicken genome (haploid GS 1.07 pg or 1.05 Gb [59]), both used as internal standards.…”

Section: Methodsmentioning

confidence: 99%

A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

et al. 2013

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“…DAPI-stained nuclei were analyzed at a flow rate of 100 nuclei/sec using a 400 nm long pass filter and chicken red blood cell (CRBC) nuclei as external standard for instrumental calibration and fluorescence reference during measurements, CRBC 2C DNA content = 2.15 pg [25]. DNA fluorescence was acquired as maximum fluorescence intensity (pulse height) and time-related parameters (fluorescence pulse area and width).…”

Section: Ploidy Estimation and Flow Cytometric Seed Screen (Fcss) Anamentioning

confidence: 99%

DNA Markers and FCSS Analyses Shed Light on the Genetic Diversity and Reproductive Strategy of Jatropha curcas L.

Ambrosi

Galla

Purelli³

et al. 2010

Diversity

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Jatropha curcas L. (2n = 2x = 22) is becoming a popular non-food oleaginous crop in several developed countries due to its proposed value in the biopharmaceutical industry. Despite the potentials of its oil-rich seeds as a renewable source of biodiesel and an interest in large-scale cultivation, relatively little is known with respect to plant reproduction strategies and population dynamics. Here, genomic DNA markers and FCSS analyses were performed to gain insights into ploidy variation and heterozygosity levels of multiple accessions, and genomic relationships among commercial varieties of Jatropha grown in different geographical areas. The determination of ploidy and the differentiation of either pseudogamous or autonomous apomixis from sexuality were based on the seed DNA contents of embryo and endosperm. The presence of only a high 2C embryo peak and a smaller 3C endosperm peak (ratio 2:3) is consistent with an obligate sexual reproductive system. Because of the lack of either 4C or 5C endosperm DNA estimates, the occurrence of gametophytic apomixis seems unlikely in this species but adventitious embryony cannot be ruled out. The investigation of genetic variation within and between cultivated populations was carried out using dominant RAPD and Inter-SSR markers, and codominant SSR markers. Nei’s genetic diversity, corresponding to the expected heterozygosity, was equal to He = 0.3491 and the fixation index as low as Fst = 0.2042. The main finding is that seeds commercialized worldwide include a few closely related genotypes, which are not representative of the original Mexican gene pool, revealing high degrees of homozygosity for single varieties and very low genetic diversity between varieties

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“…The main techniques currently employed for measuring DNA amount are flow cytometry [Geraci et al, 2007] and Feulgen image analysis densitometry (FIAD) [Hardie et al, 2002;Gregory and Shorthouse, 2003;Mendonça et al, 2010], although real time PCR has also been used [Wilhelm et al, 2003]. Doležel et al [1998] showed an excellent correspondence of genome size measurements performed by flow cytometry and FIAD.…”

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confidence: 99%

DNA Amount of X and B Chromosomes in the Grasshoppers Eyprepocnemis plorans and Locusta migratoria

Ruiz‐Ruano

Ruiz-Estévez

Rodriguez-Perez

et al. 2011

Cytogenet Genome Res

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We analyzed the DNA amount in X and B chromosomes of 2 XX/X0 grasshopper species (Eyprepocnemis plorans and Locusta migratoria), by means of Feulgen image analysis densitometry (FIAD), using previous estimates in L. migratoria as standard (5.89 pg). We first analyzed spermatids of 0B males and found a bimodal distribution of integrated optical densities (IODs), suggesting that one peak corresponded to +X and the other to –X spermatids. The difference between the 2 peaks corresponded to the X chromosome DNA amount, which was 1.28 pg in E. plorans and 0.80 pg in L. migratoria. In addition, the +X peak in E. plorans gave an estimate of the C-value in this species (10.39 pg). We next analyzed diplotene cells from 1B males in E. plorans and +B males in L. migratoria (a species where Bs are mitotically unstable and no integer B number can be defined for an individual) and measured B chromosome IOD relative to X chromosome IOD, within the same cell, taking advantage of the similar degree of condensation for both positively heteropycnotic chromosomes at this meiotic stage. From this proportion, we estimated the DNA amount for 3 different B chromosome variants found in individuals from 3 E. plorans Spanish populations (0.54 pg for B1 from Saladares, 0.51 pg for B2 from Salobreña and 0.64 for B24 from Torrox). Likewise, we estimated the DNA amount of the B chromosome in L. migratoria to be 0.15 pg. To automate measurements, we wrote a GPL3 licensed Python program (pyFIA). We discuss the utility of the present approach for estimating X and B chromosome DNA amount in a variety of situations, and the meaning of the DNA amount estimates for X and B chromosomes in these 2 species.

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DNA Content Differences Between Male and Female Chicken (Gallus gallus domesticus) Nuclei and Z and W Chromosomes Resolved by Image Cytometry

Cited by 25 publications

References 37 publications

A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

DNA Markers and FCSS Analyses Shed Light on the Genetic Diversity and Reproductive Strategy of Jatropha curcas L.

DNA Amount of X and B Chromosomes in the Grasshoppers <i>Eyprepocnemis plorans</i> and <i>Locusta migratoria</i>

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