1997
DOI: 10.1038/sj.onc.1201412
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DNA-conformation is an important determinant of sequence-specific DNA binding by tumor suppressor p53

Abstract: Sequence-speci®c transactivation of target genes is one of the most important molecular properties of the tumor suppressor p53. Binding of p53 to its target DNAs is tightly regulated, with modi®cations in the carboxyterminal regulatory domain of the p53 protein playing an important role. In this study we examined the possible in¯uence of DNA structure on sequence-speci®c DNA binding by p53, by analysing its binding to p53 consensus elements adopting di erent conformations. We found that p53 has the ability to … Show more

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Cited by 63 publications
(61 citation statements)
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References 43 publications
(63 reference statements)
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“…On the contrary, binding of p53 to some of such isoforms was inhibited by PAb421. We concluded that sequencespeci®c DNA binding of p53 can be modulated both by p53 protein conformation and by the conformation of its cognate DNA sequence (Kim et al, 1997). This ®nding opens the way for a better understanding of target selectivity of p53 sequence-speci®c transactivation, as it is becoming increasingly accepted that chromatin con®guration, speci®cally promoter DNA conformation, is an important determinant of promoter activation.…”
Section: Selectivity Of Transcriptional Regulation By P53mentioning
confidence: 75%
“…On the contrary, binding of p53 to some of such isoforms was inhibited by PAb421. We concluded that sequencespeci®c DNA binding of p53 can be modulated both by p53 protein conformation and by the conformation of its cognate DNA sequence (Kim et al, 1997). This ®nding opens the way for a better understanding of target selectivity of p53 sequence-speci®c transactivation, as it is becoming increasingly accepted that chromatin con®guration, speci®cally promoter DNA conformation, is an important determinant of promoter activation.…”
Section: Selectivity Of Transcriptional Regulation By P53mentioning
confidence: 75%
“…Sequence speci®c DNA binding by p53 also requires a certain¯exibility or secondary structures on the target DNAs and seems to be accompanied by conformational changes of p53 (Nagaich et al, 1997;Balagurumoorthy et al, 1995;Stenger et al, 1992;Kim et al, 1997). Therefore, we studied, whether these similarities between the two types of DNA interactions are re¯ected by the structural requirements on the p53 molecule.…”
Section: Discussionmentioning
confidence: 98%
“…It was found that the conformational state of the target sequence in oligos is an important determinant for p53 SSDB (Kim et al, 1997;Gohler et al, 2002). The enhanced p53 SSDB observed in long DNA molecules (Espinosa and Emerson, 2001) was assigned to a hypothetical structural changes in these molecules.…”
Section: Effect Of Supercoiling On P53 Dna Sequence-specific Bindingmentioning
confidence: 99%
“…The effect of DNA supercoiling in regulating the p53 biological activity and the SSDB has become a topic of interest only recently (Kim et al, 1997;Mazur et al, Palecek et al, 1997Palecek et al, , 1999Brazda et al, 2000;Brazdova et al, 2002;Jagelska et al, 2002) (for a review, see Hupp, 1999). In contrast to the prokaryotic genome, the eucaryotic genome was long believed to not be under superhelical stress due to accommodation of DNA writhing around histone octamers in nucleosomes (van Holde and Zlatanova, 1994;Pearson et al, 1996).…”
Section: Unconstrained Supercoiling In Eucaryotic Cellsmentioning
confidence: 99%