DNA Condensation Induced by Ruthenium(II) Polypyridyl Complexes [Ru(bpy)₂(PIPSH)]²⁺ and [Ru(bpy)₂(PIPNH)]²⁺

Abstract

Two novel DNA-intercalating ruthenium(II) complexes, [Ru(bpy)(2)(PIPSH)](2+) and [Ru(bpy)(2)(PIPNH)](2+), have been synthesized and characterized. Gel retardation assay, atomic force microscopy, and dynamic light scattering studies show that both complexes can induce the condensation of originally circular plasmid pBR322 DNA to particulate structures under neutral conditions.

Cell Cycle Arrest, Cytotoxicity, Apoptosis, DNA‐Binding, Photocleavage, and Antioxidant Activity of Octahedral Ruthenium(II) Complexes

“…2+ [24] and [Ru(phen) 2 (DBHIP)] 2+ [25] can effectively condense DNA at room temperature. To evaluate their ability to condense DNA, gel retardation of pGL3 DNA was performed in the presence of different concentrations of complexes 1, 2, and 3.…”

Cellular uptake, cytotoxicity, apoptosis, antioxidant activity and DNA binding of polypyridyl ruthenium(II) complexes

“…In recent years, the small molecules to condense DNA have been paid great attention. Complex [Ru(bpy) 2 (PIPSH)] 2þ [43] can condense DNA at a concentration of 80 mM. And complex Cu 2 (egtb)(ClO 4 ) 2 (H 2 O) 2 ] 2þ [44] can effectively and rapidly condense free DNA.…”

2-(3,5-Dibromo-4-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthrolinoruthenium(II) complexes: synthesis, characterization, cytotoxicity, apoptosis, DNA-binding and antioxidant activity

“…DNA condensation into contact structures has been received considerable attention to understand the It is well known that polyamine can effectively condense DNA (Huang et al 2006;Vijayanathan et al 2001;Sarkar et al 2009 (Sun et al 2009) can effectively condense DNA at a concentration of 80 lM. The abilities of complexes 1 and 2 to condense pGL 3 DNA were assessed by gel retardation assay.…”

“…The rich optical properties of these complexes facilitate assessments of their DNA binding capabilities as binding to DNA can be probed through changes in absorption and emission spectra. Many octahedral ruthenium(II) complexes have been proved to bind with DNA through intercalative mode (Erkkila et al 1999;Metcalfe and Thomas 2003;Zeglis et al 2007;Ghosh et al 2009;Liu et al 2009Liu et al , 2010Sun et al 2009;Tan et al 2009;Arockiasamy et al 2009;Yu et al 2009;Friedman et al 1990). These ruthenium complexes show interesting properties.…”