DNA compaction by azobenzene-containing surfactant

Zakrevskyy, Yuriy; Kopyshev, Alexey; Lomadze, Nino; Morozova, Elena; Lysyakova, Liudmila; Kasyanenko, N. A.; Santer, Svetlana

doi:10.1103/physreve.84.021909

Cited by 43 publications

(68 citation statements)

References 30 publications

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“…In this way, azobenzene containing surfactant bears an enormous potential for practical applications. One can, for instance, trigger compaction and decompaction of a DNA molecule by light in a reversible way2021222324252627282930, or remotely control the size of microgels by applying periodically UV and blue irradiation313233. One can modify polymer brushes with these molecules and make them photoresponsive with respect to topography and surface energy343536.…”

mentioning

confidence: 99%

Manipulation of small particles at solid liquid interface: light driven diffusioosmosis

Feldmann

Maduar

Santer

et al. 2016

Sci Rep

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The strong adhesion of sub-micron sized particles to surfaces is a nuisance, both for removing contaminating colloids from surfaces and for conscious manipulation of particles to create and test novel micro/nano-scale assemblies. The obvious idea of using detergents to ease these processes suffers from a lack of control: the action of any conventional surface-modifying agent is immediate and global. With photosensitive azobenzene containing surfactants we overcome these limitations. Such photo-soaps contain optical switches (azobenzene molecules), which upon illumination with light of appropriate wavelength undergo reversible trans-cis photo-isomerization resulting in a subsequent change of the physico-chemical molecular properties. In this work we show that when a spatial gradient in the composition of trans- and cis- isomers is created near a solid-liquid interface, a substantial hydrodynamic flow can be initiated, the spatial extent of which can be set, e.g., by the shape of a laser spot. We propose the concept of light induced diffusioosmosis driving the flow, which can remove, gather or pattern a particle assembly at a solid-liquid interface. In other words, in addition to providing a soap we implement selectivity: particles are mobilized and moved at the time of illumination, and only across the illuminated area.

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confidence: 99%

Manipulation of small particles at solid liquid interface: light driven diffusioosmosis

Feldmann

Maduar

Santer

et al. 2016

Sci Rep

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“…At Z = 0.33 only this peak is present in the distribution and it is mostly identical with the distribution of the colloidally stable compacted globules at the same DNA concentration. 36 The peak becomes sharper with increasing Z and stays unaffected up to the precipitation onset (Z ≈ 1.2); for Z = 1.00, it is slightly shifted to the larger sizes indicating the onset of aggregation.…”

Section: A Dna Compaction By Photosensitive Surfactantmentioning

confidence: 93%

“…First of all, the overlap concentrations of DNA in 5 mM NaCl 36 and PAA in water (calculated considering the polyelectrolyte as a random coil in a good solvent) are shown as horizontal lines. In the log-log scale the CMCs of the surfactant (in 5 mM NaCl for the complex with DNA and in pure water for the complex with PAA) are represented by the corresponding lines; all lines parallel to the CMC correspond to the respective constant surfactant concentrations.…”

Section: B Phase Diagrams Of the Complexesmentioning

confidence: 99%

“…In order to improve the complex formation, a surfactant with extended hydrophobic tail was used and the compaction could be realized at 1:1 ratio of the surfactant to the DNA charges. 36 It has been recently shown that a phase diagram for a photosensitive surfactant-DNA complex can be constructed using titration UV-visible spectroscopy experiments. 37 Very interestingly, the isothermal titration calorimetry (ITC) characterization revealed the first order transition for surfactant concentrations between the CAC (critical aggregation concentration) and the precipitation onset (complete DNA charge compensation).…”

Section: Introductionmentioning

confidence: 99%

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Interaction of photosensitive surfactant with DNA and poly acrylic acid

Zakrevskyy

Cywiński

Cywinska

et al. 2014

The Journal of Chemical Physics

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In this paper, we investigate interactions and phase transitions in polyelectrolyte-surfactant complexes formed between a cationic azobenzene-containing surfactant and two types of polyelectrolytes: natural (DNA) or synthetic (PAA: poly acrylic acid). The construction of a phase diagram allowed distancing between four major phases: extended coil conformation, colloidally stable compacted globules, colloidal instability range, and surfactant-stabilized compact state. Investigation on the complexes' properties in different phases and under irradiation with UV light provides information about the role of the surfactant's hydrophobic trans isomers both in the formation and destruction of DNA and PAA globules as well as in their colloidal stabilization. The trans isomer shows much stronger affinity to the polyelectrolytes than the hydrophilic cis counterpart. There is no need for complete compensation of the polyelectrolyte charges to reach the complete compaction. On contrary to the findings previously reported in the literature, we demonstrate - for the first time - complete polyelectrolyte compaction which occurs already at 20% of DNA (and at 50% of PAA) charge compensation. The trans isomer plays the main role in the compaction. The aggregation between azobenzene units in the photosensitive surfactant is a driving force of this process. The decompaction can be realized during UV light irradiation and is strongly influenced by the interplay between surfactant-surfactant and surfactant-DNA interactions in the compacted globules.

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“…Systems of z = 0.8 also can be compared with the system z = 0.4. It was shown earlier that the precipitation zone depends on C(DNA) [9] so the system with C(DNA) = 0.01 % at z = 0.8 is closed to the border of this zone without any turbidity in solution. The tendency to the reversibility can be manifested for some of the systems.…”

Section: Reversibility Of Transitionmentioning

confidence: 99%

DNA interaction with cis- and trans- isomers of photosensitive surfactant

Unksov¹,

Kasyanenko²

2014

J. Phys.: Conf. Ser.

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Abstract. Interaction between DNA and photosensitive cationic surfactant in a solution is studied. Studies were conducted to examine the impact of the surfactant in its cisconformation on the size of DNA molecule and also to investigate the phase behavior of the system depending on DNA and surfactant concentration. We conclude that trans-isomer of surfactant requires its smaller concentration to reach the DNA compaction compared with cisisomer received by UV radiation of solutions. Studies of DNA-surfactant systems were performed by means of spectrophotometry and viscometry. Variation of surfactant concentration enables us to determine the precipitation zone on phase diagram. From the viscosity study it can be indicated that precipitation zone is narrower for UV-radiated surfactant and it shifts to higher surfactant concentration. Also we examine the reversibility of DNA compaction in systems with the surfactant in its trans-form.

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DNA compaction by azobenzene-containing surfactant

Cited by 43 publications

References 30 publications

Manipulation of small particles at solid liquid interface: light driven diffusioosmosis

Manipulation of small particles at solid liquid interface: light driven diffusioosmosis

Interaction of photosensitive surfactant with DNA and poly acrylic acid

DNA interaction with cis- and trans- isomers of photosensitive surfactant

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