DNA characterization and polymorphism of KISS1 gene in Egyptian small ruminant breeds

Abstract

Genetic information especially of the Quantitative Trait Loci (QTL) which affect different performance traits is considered one of the most effective tools in the breeding programs of livestock. Several genes were reported as candidate genes that effect litter size performance and one of these genes is the KISS1 which is considered as a regulator of puberty onset. The polymorphisms of KISS1 gene have some relationships with high prolific and sexual precocity. The objective of this study was the detection of the restriction fragment length polymorphism (RFLP) and single nucleotide polymorphisms (SNPs) of KISS1 gene in six major Egyptian small ruminant breeds. The primers used in this study flanked a 377 bp fragment from intron 1 of KISS1 gene in sheep and goat. These PCR amplified fragments were digested with XmnI endonuclease. According to the presence or absence of the restriction site (GAANN^NNTTC) at position 121^122, we genotyped the 122 tested animals as AT (54.92%) and TT (45.08) with the absence of AA genotype. The overall frequencies of alleles A and T were 27.46 and 72.54%, respectively. The sequence analysis of purified PCR products representing these two detected genotypes declared the presence of a SNP (T→A) at position 125 in the amplified fragment which is responsible for the elimination of the restriction site and consequently the presence of two different alleles T and A. The nucleotide sequences of sheep KISS1 alleles T and A as well as goat KISS1 alleles T and A were submitted to GenBank database and have accession numbers: KP835797, KP835798, KP835799 and KP835800, respectively. It is concluded that small ruminant breeds have high frequency of KISS1 allele T which was associated with greater litter size. We recommend to increase this allele in Egyptian small ruminant breeds and also to select the animals which possess TT genotypes of KISS1 gene and enter them in breeding programs of Egyptian small ruminants to increase their fecundity traits.

Association of kisspeptin gene (KISS1) with litter size in migratory Gaddi goats in western Himalayan state of Himachal Pradesh

“…The amplified fragments obtained from all tested Gaddi goats were of 377 bp. Similar amplicon sizes were reported by An et al (2012) in 3 Chinese goat breeds, Maitra et al (2014) in 9 Indian goat breeds, Othman et al (2015) in Egyptian small ruminant breeds and El-Tarabany et al (2017) in Egyptian goat breeds. These PCR amplified fragments (377 bp) were digested with XmnI endonuclease.…”

“…Gupta et al (2015) also observed 3 digestion patterns in Black Bengal goat. Othman et al (2015) also reported similar digestion patterns in Egyptian small ruminant breeds. Similar to present investigation, polymorphism in KISS1 gene was also reported by Feng et al (2009) in Chinese goat breeds, Cao et al (2010) in Jining Grey goat, Hou et al (2011) in Chinese goat populations and Gupta et al (2015) in Black Bengal goat.…”

Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats

“…In a previous study An et al [3], it was shown that four SNPs (384G>A, 2489T>C, 2510G>A, and 2540C>T) potentially linked with litter size had been detected in Xinong Saanen goats and Guan Zhong goats, while no polymorphism was detected in exon 1 of the goat KiSS1 gene [23]. In Egyptian small ruminant breeds, the loci g2124T>A and g2270C>T were found to be significantly related to litter size [4]. However, in Indian goats, the loci G296C, G2510A, and C2540T were associated with litter size [22].…”

“…The previous studies [3,4] showed that the kisspeptin (KiSS1) gene makes a major contribution to multiparity in goats. The KiSS1 gene is situated on the long arm of chromosome 1 (1q32) [5,6] and encodes the KiSS1 (formerly known as metastin) protein [6].…”

Sequencing, polymorphism and phylogenetic characterisation of KiSS-1 gene in two Nigerian indigenous goat breeds

“…With the increasing population and demand always exceeding supply for chevon (Okewu and Iheanacho, 2015), it is necessary to exploit ways of increasing animal protein availability other than the traditional breeding method which is by evaluating genetic variation in genes relatively affecting prolificacy in goat. Genetic markers like single nucleotide polymorphisms of some genes have been reported to be significantly associated with litter size in goats such as POU class 1 homebox 1 (POU1F1) gene (Feng et al, 2011), KiSS-1 gene (Cao et al, 2010;An et al, 2013a,b;Othman et al, 2015;Mekuriaw et al, 2017) Gonadotropin-releasing hormone receptor (GnRHR) gene (Yang et al, 2011;Huang et al, 2012;Bemji et al, 2018), KIT ligand gene (An et al, 2011;2015;, Inhibin alpha (INHA) gene (An et al, 2012;Sharma et al, 2015;Isa et al, 2017). Although characterization of genetic variation in Nigerian goat breeds have been carried out using genetic markers like Microsatellite, Mitochondrial, Biochemical and SNPs (Shoyombo et al, 2015;Awotunde et al, 2015;Ojo et al, 2017;Bemji et al, 2018;Isa et al, 2019), there has been no published information on KiSS-1 gene variant of Nigerian goat breeds.…”