DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips

Hu, Mei; He, Yao; Song, Shiping; Yan, Juan; Lu, Hao‐Ting; Weng, Lixing; Wang, Lianhui; Fan, Chunhai

doi:10.1039/c0cc01608j

Cited by 32 publications

(28 citation statements)

References 39 publications

(44 reference statements)

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“…Aqueous QDs could bind to biomoleculars through electrostatical adsorption, coordinated compound, biotin-avidin interactions, covalent cross-linking (amine and carboxylic groups, amine and sulfhydryl groups, or aldehyde and hydrazide functions), or polyhistidine tags [146–151]. Our group developed a DNA-bridged strategy to facilely conjugate streptavidin (STV) to QDs, leading to a convenient and stable QD-DNA-biotin-STV conjugate, which served as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers [147]. These QD-based bioconjugates have been applied into ELISA, western blot, and microarray, and so on [152–154].…”

Section: Biosensorsmentioning

confidence: 99%

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Early Lung Cancer Diagnosis by Biosensors

Zhang

Yang

Weng

et al. 2013

IJMS

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Lung cancer causes an extreme threat to human health, and the mortality rate due to lung cancer has not decreased during the last decade. Prognosis or early diagnosis could help reduce the mortality rate. If microRNA and tumor-associated antigens (TAAs), as well as the corresponding autoantibodies, can be detected prior to clinical diagnosis, such high sensitivity of biosensors makes the early diagnosis and prognosis of cancer realizable. This review provides an overview of tumor-associated biomarker identifying methods and the biosensor technology available today. Laboratorial researches utilizing biosensors for early lung cancer diagnosis will be highlighted.

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Section: Biosensorsmentioning

confidence: 99%

“…The microfluidic chip combines the high-throughput capabilities of a microfluidic network with the high sensitivity and multicolor imaging ability of QDs [148]. The QD-DNA-biotin-STV conjugates mentioned previously were also applied for ultrasensitive detection of cancer biomarkers within a microfluidic protein chip [147]. …”

Section: Biosensorsmentioning

confidence: 99%

Early Lung Cancer Diagnosis by Biosensors

Zhang

Yang

Weng

et al. 2013

IJMS

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“…Fan et al fabricated QD-DNA-biotinstreptavidin conjugates that serve as fluorescent immunoprobes for ultrasensitive detection of cancer biomarkers carcinoembryonic antigen [127]. Meanwhile, Wang et al developed multiplexed assay microfluidic protein chip based on the fluorescent immunoprobes [128].…”

Section: Protein and Enzymementioning

confidence: 99%

Fluorescent nanoparticles for chemical and biological sensing

Liu

Yang

et al. 2011

Sci. China Chem.

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Fluorescent nanoparticles (NPs), including quantum dots (QDs), dye-doped NPs, and rare earth-based NPs, etc., have been a major focus of research and development during the past decade. The impetus behind such endeavors can be attributed to their unique chemical and optical properties, such as bright fluorescence, high photostability, large Stocks shift and flexible processability. The introduction of fluorescent NPs into analytical chemistry has opened up new venues for fluorescent analysis. In this review, we focus on the developments and analytical applications of fluorescent NPs in the chemical and biological sensing of pH, ions, organic compounds, small biological molecules, nucleic acids, proteins, virus and bacteria. The review also points out the in vitro and in vivo imaging application of fluorescent NPs at the cell and body levels. Meanwhile, the advantages of NPs brought field of sensing and signal transductions are also discussed. fluorescence, sensing, quantum dots, dye-doped nanoparticles, rare earth-based nanoparticles

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“…Hu et al are able to achieve an even lower limit of detection for CEA (50 fM) in an analogous assay. 182 …”

Section: Detection Of Extracellular Cancer Biomarkersmentioning

confidence: 99%