DNA Biosensors Based on Nanostructured Materials

Ferancová, Adriana; Labuda, Ján

doi:10.1002/9783527621507.ch11

Cited by 15 publications

(14 citation statements)

References 163 publications

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“…5,6 To ensure sensitivity of the electrochemical detection, nanostructured interface is frequently used. 6,10 The aim of this study is to find procedures allowing a simple and effective combination of the electrochemical and electric detection modes mentioned above to evaluate dsDNA damage caused by reactive oxygen species formed via Fenton reaction and by the Cu 2+ -quercetin system. 9 Regarding the DNA damage detection, we have recently fabricated and characterized carbon paste-based screen-printed electrodes covered by the interface of various types of electroconductive carbon nanotubes.…”

Section: Introductionmentioning

confidence: 99%

Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode

Ziyatdinova

Labuda

2011

Anal. Methods

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DNA biosensor (DNA/SWCNT-COOH-CHIT/SPCE) composed of dsDNA adsorptive layer on a carboxylated single-walled carbon nanotubes-chitosan composite deposited at a commercial carbon based screen-printed electrode has been prepared and applied to a complex investigation of damage to DNA by the Fenton type cleavage agent (hydroxyl radicals formed in the mixture of Cu 2+ , H 2 O 2 and ascorbic acid) and copper(II)-quercetin system in 0.1 M PBS pH 7.0 under aerobic conditions. The dsDNA damage detection is performed by using square-wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1 Â 10 À7 M thioridazine and 1 Â 10 À3 M K 4 [Fe(CN) 6 ]/K 3 Fe(CN) 6 in the 0.1 M phosphate buffer solution, pH 7.0. Initial enhancement of the intrinsic guanine and adenine moieties SWV response over that of original dsDNA one indicates opening of the helix structure in the first stage of damage. At the same time, decrease in the intercalated thioridazine response confirms damage of the helix structure in parallel to deep degradation of the DNA chain and its removal from the electrode surface as indicated by the CV and EIS measurements in the presence of the [Fe(CN) 6 ] 3À/4À redox indicator in solution.

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Section: Introductionmentioning

confidence: 99%

Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode

Ziyatdinova

Labuda

2011

Anal. Methods

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“…Carbon paste and other composite electrodes present a large number of advantages over other working electrodes, like low background currents and easy renewal [7]. For that reason composites based on the use of carbon nanotubes have received much attention [13].…”

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confidence: 99%

“…For that reason composites based on the use of carbon nanotubes have received much attention [13]. A variety of binders such as mineral oil or nujol, Teflon and bromoform can be used to produce CNT pastes or composites [7].…”

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confidence: 99%

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Recent Advances of Sensitive Electroanalytical Tools and Probes in the Study of DNA Structure

Girousi¹,

Serpi²,

Karastogianni³

et al. 2010

COC

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The research and development of electrochemical biosensors applied to life sciences during the past years were reviewed in the present article. In the first part of the article, a brief introduction on nanomaterial based electrochemical DNA biosensors is given. In the second part DNA biosensors and transition metal complexes with biological interest are described. Finally, the development trends of electrochemical biosensors in genetic and epigenetic testing are being discussed.

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“…Nanotechnology-enabled sensors are already widely used in the field of biosensors including NA-based sensors [42][43][44]. Gold nanoparticles [28] and carbon nanomaterials, particularly CNTs [45], have attracted attention due to their unique structural, electronic, mechanical, and chemical properties.…”

Section: Commentmentioning

confidence: 99%

Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

Labuda

Oliveira-Brett

Evtugyn

et al. 2010

Pure and Applied Chemistry

213

113

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An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electrochemical NA biosensors, including their critical evaluation, which should be valuable for a wide audience, from academic, biomedical, environmental, and food-testing, drug-developing, etc. laboratories to sensor producers.

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DNA Biosensors Based on Nanostructured Materials

Cited by 15 publications

References 163 publications

Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode

Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode

Recent Advances of Sensitive Electroanalytical Tools and Probes in the Study of DNA Structure

Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

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