DNA Biosensors-A Review

Kavita, .

doi:10.4172/2155-9538.1000222

Cited by 27 publications

(10 citation statements)

References 48 publications

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“…Finally, the reader instrument translates the signal to a measurable value which can be easily determined by the user (Figure 1). In the medical field, biosensors eliminate the need for sample preparation and, as a result, help reduce cost, increase testing efficiency and aid in onsite diagnosis, which helps communities living in remote areas and are cut off from access to the medical facilities which are generally clustered around urban areas (V, 2017).…”

Section: Potential Of Electrochemical Deoxyribonucleic Acid Biosensor...mentioning

confidence: 99%

DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

Stephen

Suchanti

Jain

et al. 2022

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Purpose Neglected tropical diseases (NTDs) are a set of infectious diseases that primarily affect low-income countries situated near the equator. Effective diagnostic tools hold the key to stemming the spread of these infectious diseases. However, specificity is a major concern associated with current diagnostic protocols. In this regard, electrochemical deoxyribonucleic acid (DNA) biosensors could play a crucial role, as highlighted by renewed interest in their research. The purpose of this study was to highlight the current scenario for the design and development of biosensors for the detection of NTDs related pathogens. This review highlights the different types of factors involved and the modifications used to enhance sensor properties. Design/methodology/approach The authors discuss the potential of electrochemical DNA biosensors as efficient, affordable diagnostic tools for the detection of pathogens associated with NTDs by reviewing available literature. This study discusses the biosensor components, mainly the probe selection and type of electrodes used, and their potential to improve the overall design of the biosensor. Further, this study analyses the different nanomaterials used in NTD-based electrochemical DNA biosensors and discusses how their incorporation could improve the overall sensitivity and specificity of the biosensor design. Finally, this study examines the impact such techniques could have in the future on mass screening of NTDs. Findings The findings provide an in-depth analysis of electrochemical DNA biosensors for the detection of pathogens associated with NTDs. Originality/value This review provides an update on the different types and modifications of DNA biosensors that have been designed for the diagnosis of NTD-related pathogens.

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Section: Potential Of Electrochemical Deoxyribonucleic Acid Biosensor...mentioning

confidence: 99%

DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

Stephen

Suchanti

Jain

et al. 2022

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“…Selective electrochemical biosensors are obtained by employing nucleic acid sequences as specific recognition elements. Biosensors based on nucleic acids have been widely explored over the last years due to their manifold physical, chemical, and biological properties [58]. Among them, aptasensors have been widely explored [59], basing on the use of aptamers, namely small artificial sequences of single-stranded DNA or RNA (ssDNA or ssRNA) capable of selectively recognize the specific target, including proteins, peptides, and various small molecules of analytical interest [60].…”

Section: Origami Paper-based Electrochemical Dna Sensorsmentioning

confidence: 99%

Origami Paper-Based Electrochemical (Bio)Sensors: State of the Art and Perspective

et al. 2021

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In the last 10 years, paper-based electrochemical biosensors have gathered attention from the scientific community for their unique advantages and sustainability vision. The use of papers in the design the electrochemical biosensors confers to these analytical tools several interesting features such as the management of the solution flow without external equipment, the fabrication of reagent-free devices exploiting the porosity of the paper to store the reagents, and the unprecedented capability to detect the target analyte in gas phase without any sampling system. Furthermore, cost-effective fabrication using printing technologies, including wax and screen-printing, combined with the use of this eco-friendly substrate and the possibility of reducing waste management after measuring by the incineration of the sensor, designate these type of sensors as eco-designed analytical tools. Additionally, the foldability feature of the paper has been recently exploited to design and fabricate 3D multifarious biosensors, which are able to detect different target analytes by using enzymes, antibodies, DNA, molecularly imprinted polymers, and cells as biocomponents. Interestingly, the 3D structure has recently boosted the self-powered paper-based biosensors, opening new frontiers in origami devices. This review aims to give an overview of the current state origami paper-based biosensors, pointing out how the foldability of the paper allows for the development of sensitive, selective, and easy-to-use smart and sustainable analytical devices.

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“…These findings would facilitate more precise Cas9 engineering in future. Nowadays, DNA-biosensors are intensively applied for resolution of actual bio-analytical and medical problems, such as identification of biological material by primary sequence of nucleotides, improvement of early diagnostic and treatment methods of oncological diseases, determination of pharmacological preparations of anti-cancerous action (Kavita, 2017). Application of DNA-biosensors significantly decreases both analysis cost and time of its carrying out as compared to traditional medico-biological methods.…”

Section: Liangzhen Zheng and Yuguang Mumentioning

confidence: 99%

Book of Abstracts. Albany 2019: The 20th Conversation

Sarma¹

2019

Journal of Biomolecular Structure and Dynamics

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The aim of structural biology is to explain life processes in terms of macromolecular interactions in the cell. These interactions typically involve more than two partners, and can run up to dozens. A full description will need to characterize all structures on the atomic level, and the way these structures change in the process. Because of the crowded environment of the cell, such characterization is presently (but see below) only possible when the group of interacting molecules (often organized into processive 'molecular machines') is isolated and studied in vitro. While X-ray crystallography has provided structures of a large number of molecular structures, the need for crystals diffracting to high resolution has severely limited the number of supramolecular assemblies and the range of conformers that can be studied with this technique. Single-particle cryo-electron microscopy is about to fill this gap, allowing functional processes to be studied in great detail without imposing restraints on the structures. There are many examples now for this expansion of Structural Biology toward a full characterization of a functional process. Future developments of single-particle cryo-EM include the study of short-lived intermediates in a nonequilibrium system by time-resolved techniques, and the characterization of continuous structural changes using data mining from large ensembles of molecule images. It is very interesting and promising that another technique of cryo-EM, cryo-electron tomography of FIB-milled cell sections, has started to contribute information about processes even within the cellular context. Distinct ribosome states, for instance, have already been identified and localized by subtomogram averaging. With this advance, we get closer to the fulfillment of the most ambitious aim of Structural Biology, the visualization and interpretation of molecular interactions in situ.

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DNA Biosensors-A Review

Cited by 27 publications

References 48 publications

DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

DNA biosensor based detection for neglected tropical disease: moving towards smart diagnosis

Origami Paper-Based Electrochemical (Bio)Sensors: State of the Art and Perspective

Book of Abstracts. Albany 2019: The 20th Conversation

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