DNA Biosensor for the Detection of Hydrazines

Abstract

A double-stranded (ds) DNA-coated carbon paste electrode is employed as a remarkably sensitive biosensor for the detection of hydrazine compounds. The sensor relies on monitoring changes in the intrinsic anodic response of the surface-confined DNA resulting from its interaction with hydrazine compounds and requires no label or indicator. Short reaction times (1-10 min) are sufficient for monitoring part-per-billion levels of different hydrazines. Applicability to untreated natural water samples is illustrated. The response mechanism is discussed, along with prospects of using DNA biosensors for quantitaing other important molecules and elucidating DNA interactions and damage.

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

“…17,[87][88][89][90][91][92] Changes in the DNA redox properties were reported for studies of interaction between DNA and analyte. 88,89,91 Moreover, the amount of electro-active substrate trapped on the DNA layer was investigated.…”

“…17,[87][88][89][90][91][92] Changes in the DNA redox properties were reported for studies of interaction between DNA and analyte. 88,89,91 Moreover, the amount of electro-active substrate trapped on the DNA layer was investigated. 87 Thus, it was concluded that electrochemical biosensors offered an easier immobilization of the DNA layer and faster measurement Flow injection analysis with an evanescent wave biosensor was coupled for the detection of DNA intercalator on the basis of their different affinities for double-stranded DNA at a constant concentration of the ethidium bromide.…”

“…88 The DNA biosensor was based on the diminution of the guanine oxidation peak for the detection of hydrazine.…”

“…89,91 In contrast, only a few studies were devoted for the design of environmental DNA biosensors. 88,93 Two kinds of disposable DNA electrochemical biosensors were suggested for DNA hybridization and toxic compound detections. 91 It was observed that the single-use biosensor with screen-printed electrode (SPE) avoided contamination among samples and allowed constant sensitivity and reproducibility.…”

Eletrodos modificados com DNA: uma nova alternativa em eletroanálise

“…Diversos são os materiais de eletrodo que podem ser modificados com DNA, vale dizer, mercúrio 8,[91][92][93][94][95] , ouro [96][97][98][99][100][101] , grafite 95,102 , pasta de carbono 89,[103][104][105][106][107][108][109][110][111][112][113][114][115] e carbono vítreo 47,80,100,[116][117][118][119][120][121][122][123] . Muitos destes exemplos já estão sendo, genericamente, denominados biossensores eletroquímicos de DNA ou genossensores 78 .…”