DNA binding proteins of Schistosoma mansoni recognizing a hexanucleotide motif occurring in genes regulated by steroids

Rumjanek, Franklin David; Braga, Vania; Kelly, Charles

doi:10.1016/0305-0491(89)90170-3

Cited by 3 publications

(1 citation statement)

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“…haematobium (Bobek et al 1989). The downstream, non-translated region of S. mansoni gene F-10 (Simpson et al 1987;Kunz et al 1987), displays a hexanucleotide and pentanucleotide sequence identical to hormone regulatory elements (HRE) present in genes regulated by steroids (Rumjanek, Braga & Kelly, 1989). These results hint at the association between steroid receptor proteins and the regulation of the transcription of the eggshell proteins.…”

Section: Introductionmentioning

confidence: 99%

F-10 nuclear binding proteins ofSchistosoma mansoni: structural and functional features

et al. 1995

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By incubating total protein extracts of Schistosoma mansoni with 3H-17-beta-estradiol and 20-hydroxyecdysone, steroid binding proteins were detected in both male and female worms. The interaction of nuclear proteins with a restriction fragment of the gender and stage-specific gene F-10 was investigated using the 'band-shift' technique. Male and female nuclear proteins bound in a distinct way to the fragment of this gene containing putative regulatory consensus motifs. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the oestrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females a profile similar to that obtained with male worm protein extracts. This effect of Tamoxifen could not be correlated to inhibition of protein biosynthesis. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.

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Section: Introductionmentioning

confidence: 99%

F-10 nuclear binding proteins ofSchistosoma mansoni: structural and functional features

et al. 1995

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Protein interactions with a gender-specific gene ofSchistosoma mansoni: characterization by DNase I footprinting, band shift and UV cross-linking

1993

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A schistosome gender specific gene (F-10), was used as a probe to characterize DNA binding proteins from adult male and female Schistoma mansoni. Using the band-shift and DNase I footprinting methods, it was found that proteins from male and female worms bound to the intact F-10 gene and to restriction fragments corresponding to different domains of the gene, generating relatively long protected sites. Clear differences between male and female proteins were only observed when nuclear proteins were tested. Thus, gender-specific binding was detected in fragments corresponding to the 5' and 3' ends. UV-induced cross-linking between schistosome proteins and a synthetic oligonucleotide bearing a steroid response element present in the 3' untranslated end of the F-10 gene, revealed a major DNA binding protein with a molecular mass of 30 kDa, in both male and female worms. These results suggested that the activation of transcription of the F-10 gene may depend essentially on nuclear proteins.

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Molecular aspects of sexual development and reproduction in nematodes and schistosomes

Boag

Newton²,

Gasser

2001

Advances in Parasitology Volume 50

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DNA binding proteins of Schistosoma mansoni recognizing a hexanucleotide motif occurring in genes regulated by steroids

Cited by 3 publications

References 14 publications

F-10 nuclear binding proteins ofSchistosoma mansoni: structural and functional features

F-10 nuclear binding proteins ofSchistosoma mansoni: structural and functional features

Protein interactions with a gender-specific gene ofSchistosoma mansoni: characterization by DNase I footprinting, band shift and UV cross-linking

Molecular aspects of sexual development and reproduction in nematodes and schistosomes

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