DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole

Abstract

Abstract:The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of K b = 1.05ˆ10 5 M´1. TCPC also displayed good photonuclease activity, which involves singlet oxygen species ( 1 O 2 ). The binding constant between TCPC and human serum albumin (HSA) is K A = 2.24ˆ10 5 M´1 with a simulated binding distance of 2.06 nm. The fluorescence quenching of HSA by TCPC followed a static quenching process. Site marker competitive displacement experiments indicated that warfarin site I is the main binding site. The secondary structure of HSA was changed upon interaction with TCPC, which was confirmed by UV-Visible and CD spectroscopy.

Potent and Selective Cytotoxic and Anti‐inflammatory Gold(III) Compounds Containing Cyclometalated Phosphine Sulfide Ligands

“…However,t he maximum emissionw avelength of HSA did not change during the interaction with complexes 2, 3,a nd 5,w hich suggests that the complexes interacted with the hydrophobic region located inside HSA withouta ltering the polarity around the tryptophan residues.I nc ontrast, ar edshift of the emission maximum in the fluorescences pectrum of the albumin was observed after incubation with complex 4, which suggests an increased hydrophobicity aroundt he tryptophanresidues. [25] In vitro anticancera ctivity…”

Synthesis, Characterization, DNA/HSA Interactions, and Anticancer Activity of Two Novel Copper(II) Complexes with 4-Chloro-3-Nitrobenzoic Acid Ligand

“…Hence, intercalative binding occurred between the DNA and each complex. The intrinsic binding constant ( K b ) was computed as shown in Equation (1) below [ 37 ]: where ε a designates the apparent complex extinction coefficient, ε f designates free complex extinction coefficient, ε b designates the bound complex extinction coefficient, and [DNA] designates the CT-DNA quantity. In the [DNA]/( ε a − ε f ) vs. [DNA] plot, K b is the slope: intercept ratio.…”

Modifications of expression of genes and proteins involved in DNA repair and nitric oxide metabolism by carbatonides [disodium-2,6-dimethyl-1,4-dihydropyridine- 3,5-bis(carbonyloxyacetate) derivatives] in intact and diabetic rats

“…All five compounds were capable of interacting with DNA, although their affinity to DNA was lower compared to other 1,4-DHPs (10,12). The increase in fluorescence of carbatone and propcarbatone in the presence of DNA indicates a probable intercalation mechanism; the quenching of fluorescence by metcarbatone and etcarbatone indicates binding to the outer surface of DNA molecule (18). Interestingly, styrylacarbatone manifested a higher affinity to DNA, although DNA quenched its fluorescence, ethidium bromide extrusion assay indicates intercalation.…”