DNA binding and antiradical potential of ethyl pyruvate: Key to the DNA radioprotection

Sharma, Deepti; Singh, Anju; Pathak, Mallika; Kaur, Lajpreet; Kumar, Vinod; Roy, Bal Gangadhar; Ojha, Himanshu

doi:10.1016/j.cbi.2020.109313

Cited by 14 publications

(5 citation statements)

References 37 publications

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“…2) showed that 25 112 results were annotated and divided into three functional groups, namely, biological process (33.52%), molecular function (28.65%) and cell component (37.83%). The 10 most significant differences were DNA binding, transcription DNA-templated, zinc ion binding, G2/M transition of mitotic cell cycle, adhesion of symbiont to host, tryptophan biosynthetic process, asexual spore wall assembly, differentially expressed genes are closely related to cell stress resistance, proliferation, tissue repair, adhesion and cell structure (Zámborszky, 2013;Swirnoff et al, 1995;Sharma et al, 2020). Results did not clearly reflect or speculate the existence of the gossypol-degrading gene in AN.…”

Section: Fig 2 Go Functional Annotation Of Differentially Expressed Genes Under the Gossypol Stressmentioning

confidence: 84%

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Zhang

Shu

Wang

et al. 2021

Preprint

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The effectiveness of the classic screening strategy was verified, by duplicating and verifying the degradation of gossypol by the Aspergillus niger. It can reduce the free gossypol content through biosorption but has no effect on the total gossypol content and cannot effectively degrade gossypol. And the most interesting thing we found the strain can secrete agarase, utilise agar as carbon source. In this case, that will mislead researchers and lead them to make wrong judgments. That turns out the usual methods of previous screening strategies are not rigorous enough, the classic screening method has defect in screening toxin-degrading strain, so agar control group should be added. In this study, some suggestions are put forward to optimise the same type of experiments and broaden the idea of detoxification by microorganisms and provide reference for screening effective toxin-degrading microorganisms.

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Section: Fig 2 Go Functional Annotation Of Differentially Expressed Genes Under the Gossypol Stressmentioning

confidence: 84%

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Zhang

Shu

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…showed that 25 112 results were annotated and divided into three functional groups, namely, biological process (33.52%), molecular function (28.65%) and cell component (37.83%). The 10 most signi cant differences were DNA binding, transcription DNA-templated, zinc ion binding, G2/M transition of mitotic cell cycle, adhesion of symbiont to host, tryptophan biosynthetic process, asexual spore wall assembly, differentially expressed genes are closely related to cell stress resistance, proliferation, tissue repair, adhesion and cell structure[17][18][19]. Results did not clearly re ect or speculate the existence of the gossypol-degrading gene in AN.…”

mentioning

confidence: 81%

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Zhang

Shu

Wang

et al. 2021

Preprint

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Background The gossypol, as an antinutritional factor or natural plant toxin, has antifertility effects on humans and animals, exists in cottonseed oil and meal, and is a typical food or feed contaminant. It is the classic screening method to using acetate gossypol as the sole carbon sources to prepare selective medium and screening gossypol-degrading microorganisms. By the classic screening strategy, many researchers have discovered that some microorganisms, and Aspergillus niger is the most reported microorganism. A strain of A. niger which can grow in an agar solid medium with gossypol as the sole carbon source is isolated from cattle rumen liquid was obtained by classic screening strategy. The effectiveness of the classic screening strategy was verified, by duplicating and verifying the degradation of gossypol by the A. niger. Results the A. niger can reduce the free gossypol (FG) content through biosorption but has no effect on the total gossypol (TG) content. And it can secrete agarase, utilise agar as carbon source. Conclusions The A. niger can secrete agarase, but cannot effectively degrade gossypol, In this case, it will mislead researchers and lead them to make wrong judgments. The usual methods of previous screening strategies are not rigorous enough, the classic screening method has defect in screening toxin-degrading strain, so agar control group should be added.

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“…The direct mechanism of action is determined by the direct interaction of endogenous or exogenous factors with the DNA molecule, which leads to the breakdown of chemical bonds at the DNA level and initiates changes in its spatial structure [44,45]. The mechanism of mediated action by exogenous and endogenous factors is realized through their metabolism and activation of intermediate products, whose interaction with DNA underlies its damage [46,47]. While exogenous and endogenous factors have a great potential for modifying genetic information, the relative contribution of internal and external factors to the incidence of cancer remains uncertain [6].…”

Section: Sources and Types Of Dna Damagementioning

confidence: 99%

Systems for Genetic Assessment of the Impact of Environmental Factors

Kislyak,

Dugan,

Yalovenko

2024

Innov Biosyst Bioeng

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One of the most important components of environmental protection is the development of hygiene standards aimed at shielding the human population from the adverse effects of environmental pollution. The European and American Chemical Societies have reported approximately 800,000 chemicals, with no available information on potential risks to human genetic health and negative environmental impact. Given the exponential increase in chemical compounds generated by humanity in various industries, the issue of effectivly identifying and accounting for various genetic and carcinogenic hazards is particularle relevant. The assessment of potential genotoxicity of environmental factors is an integral part of genetic safety assessment for both prokaryotic and eukaryotic organisms, including humans. The evaluation of the genetic activity of chemical compounds is a fundamentsl requirement for their comprehensive toxicological assessment. From the perspective of genetic and epigenetic mechanisms of influence, our review considers standard methods for detecting and assessing the potential genetic hazard associated with environmental factors. These methods are part of a standard, generally accepted test system battery. Additionally, the review covers some modern experimental methods that are not widely accepted today. A detailed analysis of approaches to the assessment of potential genetic mutagenic activity was carried out, presenting their main advantages and disadvantages. Taking into account the recommendations issued by the Organisation for Economic Co-operation and Development on testing hazardous chemical compounds that may affect human health, an attempt was made to find optimal approaches to solving the task of predicting genetic effects and their consequences for humans.

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DNA binding and antiradical potential of ethyl pyruvate: Key to the DNA radioprotection

Cited by 14 publications

References 37 publications

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

Systems for Genetic Assessment of the Impact of Environmental Factors

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