DNA binding and antibacterial properties of ternary lanthanide complexes with salicylic acid and phenanthroline

Abstract

Twelve ternary lanthanide complexes RE(sal)3phen (RE3+ = La3+, Pr3+, Nd3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Tm3+, Yb3+, Lu3+, sal = salicylic acid, phen = phenanthroline) were prepared. Interactions between the complexes and calf thymus DNA (ct‐DNA) were investigated using UV–visible spectrophotometry, fluorescence quench experiment and viscosity measurement. Hypochromicity and red shift of the absorption spectra of complexes were observed in the presence of DNA. The enhanced emission intensity of ethidium bromide (EB) in the presence of DNA was quenched by the addition of lanthanide complexes, which indicated that the lanthanide complexes displaced EB from its binding sites in DNA. Based on the systematic research of the binding constant (Kb) and the fluorescence quenching constant (Kq) of the 12 complexes, we found that the complexes with smaller lanthanide ion radius had stronger binding abilities with DNA. Viscosity measurement showed that the relative viscosity of the DNA solution was enhanced with increasing the amounts of the complexes. All these results suggested that the complexes could bind to DNA and the major binding mode was intercalative binding. Moreover, all these complexes exhibited excellent antibacterial abilities against Escherichia coli. Also, the antibacterial activities of complexes with heavy rare earth were higher than those of complexes with light rare earth. Copyright © 2014 John Wiley & Sons, Ltd.

Lanthanum induced B-to-Z transition in self-assembled Y-shaped branched DNA structure

“…Though condensation was recorded upto 10 mM of REEs, invariably the process slowed down after 5 mM of REEs. Commonly, binding strength between DNA and complexes parallels the magnitude of the hypochromism42434445. Interestingly, the hypochromicity and red-shift emerging in the absorbance spectra advocates for strong binding of the REEs to the bDNA structures (Figs S3–S7)42434445.…”

“…Commonly, binding strength between DNA and complexes parallels the magnitude of the hypochromism42434445. Interestingly, the hypochromicity and red-shift emerging in the absorbance spectra advocates for strong binding of the REEs to the bDNA structures (Figs S3–S7)42434445. While other bDNA structures showed typical condensation by interaction with La 3+ , Gd 3+ or Dy 3+ , with increasing the concentration of LaCl 3 , US2 bDNA showed a dramatic alteration of the spectra with a characteristic positive peak at 262 nm and a negative peak at 298 nm, that is indicative of B-Z transition (Fig.…”

Probing the biomolecular (DNA/BSA) interaction by new Pd(II) complex via in-depth experimental and computational perspectives: synthesis, characterization, cytotoxicity, and DFT approach

“…Thus, the binding mode of the [Pd(bpy)(proli-dtc)]NO 3 complex to CT-DNA is likely to be intercalation, which is aligned with the finding of UV-Vis experiment and can be further verified via viscosity measurements too. The aforementioned methods have been applied for some metal complexes [72]. BSA structure possesses three intrinsic fluorophores, namely phenylalanine, tyrosine and tryptophan.…”

Produção de compostos de coordenação como ação de permanência e êxito dos estudantes do curso de licenciatura em química/ Production of coordination compounds as action of permanence and success of students in the chemistry license course

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