DNA-based screening for an intracellular cadherin mutation conferring non-recessive Cry1Ac resistance in field populations of Helicoverpa armigera

Zhang, Haonan; Tang, Manshu; Yang, Fan; Yang, Yihua; Wu, Yi

doi:10.1016/j.pestbp.2013.06.007

Cited by 23 publications

(26 citation statements)

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“…To put the diversity of pink bollworm cadherin from India in perspective, we note that only 22 cadherin resistance alleles have been reported previously based on more than a decade of work by several research teams analyzing thousands of individuals representing three major cotton pests. These previously reported cadherin resistance alleles consist of the four in pink bollworm from Arizona [38], [42], one in H. virescens from the southeastern United States [64], and 17 in H. armigera from northern China and western India [19]–[23], [63], [65]–[66]. Mis-splicing was reported for one cadherin resistance allele from pink bollworm [62] and another from H. armigera [63], as noted above, but not for the other previously reported cadherin alleles.…”

Section: Discussionmentioning

confidence: 97%

“…Although alternative splicing generated five cadherin isoforms in a Cry1Ac-resistant strain of T. ni [61], resistance in this strain is genetically linked with the ABCC2 gene, and is not associated with variation in either the transcripts or gDNA for cadherin [18], [55]. However, mutations in cadherin gDNA of pink bollworm and H. armigera that cause mis-splicing and produce a single altered transcript linked with resistance to Cry1Ac have been reported [62]–[63]. In H. armigera , four different indels in gDNA yield the same altered cDNA transcript that lacks exon 32 [63].…”

Section: Discussionmentioning

confidence: 98%

“…However, mutations in cadherin gDNA of pink bollworm and H. armigera that cause mis-splicing and produce a single altered transcript linked with resistance to Cry1Ac have been reported [62]–[63]. In H. armigera , four different indels in gDNA yield the same altered cDNA transcript that lacks exon 32 [63]. For the previously characterized pink bollworm cadherin resistance allele r3 , insertion of a non-LTR chicken-repeat retrotransposon ( CR1-1_Pg ) causes splicing out of exon 21 from mRNA [62].…”

Section: Discussionmentioning

confidence: 99%

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Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India

et al. 2014

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Evolution of resistance by insect pests can reduce the benefits of insecticidal proteins from Bacillus thuringiensis (Bt) that are used extensively in sprays and transgenic crops. Despite considerable knowledge of the genes conferring insect resistance to Bt toxins in laboratory-selected strains and in field populations exposed to Bt sprays, understanding of the genetic basis of field-evolved resistance to Bt crops remains limited. In particular, previous work has not identified the genes conferring resistance in any cases where field-evolved resistance has reduced the efficacy of a Bt crop. Here we report that mutations in a gene encoding a cadherin protein that binds Bt toxin Cry1Ac are associated with field-evolved resistance of pink bollworm (Pectinophora gossypiella) in India to Cry1Ac produced by transgenic cotton. We conducted laboratory bioassays that confirmed previously reported resistance to Cry1Ac in pink bollworm from the state of Gujarat, where Bt cotton producing Cry1Ac has been grown extensively. Analysis of DNA from 436 pink bollworm from seven populations in India detected none of the four cadherin resistance alleles previously reported to be linked with resistance to Cry1Ac in laboratory-selected strains of pink bollworm from Arizona. However, DNA sequencing of pink bollworm derived from resistant and susceptible field populations in India revealed eight novel, severely disrupted cadherin alleles associated with resistance to Cry1Ac. For these eight alleles, analysis of complementary DNA (cDNA) revealed a total of 19 transcript isoforms, each containing a premature stop codon, a deletion of at least 99 base pairs, or both. Seven of the eight disrupted alleles each produced two or more different transcript isoforms, which implicates alternative splicing of messenger RNA (mRNA). This represents the first example of alternative splicing associated with field-evolved resistance that reduced the efficacy of a Bt crop.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India

et al. 2014

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“…Produtores de algodão no Sul da Bahia tiveram 80% de suas plantações destruídas por essa praga (Czepak et al, 2013), que, por ser altamente polífaga, também atacou outras culturas na região Nordeste, Centro-Oeste e Sul do País (Ávila et al, 2013;Specht et al, 2013). Na Austrália, na Índia e na China, H. armigera é considerada a praga de maior impacto econômico (Liao et al, 2002;Ibargutxi et al, 2008;Zhang et al, 2013), e a severidade do ataque está relacionada a seu hábito alimentar polífago, a sua boa capacidade de dispersão e a sua adaptação a diferentes ambientes (Tay et al, 2013;Leite et al, 2014).…”

Section: Introductionunclassified

“…agropec. bras., Brasília, v.50, n.11, p.999-1005, nov. 2015 DOI: 10.1590/S0100-204X2015001100002 de proteínas dessa bactéria, tóxicas a H. armigera, especialmente em países como China, Austrália e Índia (Liao et al, 2002;Zhang et al, 2013).…”

Section: Introductionunclassified

Toxicidade e capacidade de ligação de proteínas Cry1 a receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae)

Sebastião

Lemes

Figueiredo

et al. 2015

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a toxicidade e a capacidade de ligação das proteínas Cry1Aa, Cry1Ab, Cry1Ac e Cry1Ca, de Bacillus thuringiensis, a receptores intestinais de Helicoverpa armigera. Realizou-se análise de ligação das proteínas ativadas às vesículas de membrana da microvilosidade apical (VMMA) do intestino médio de H. armigera, além de ensaios de competição heteróloga para avaliar sua capacidade de ligação. Cry1Ac destacou-se como a proteína mais tóxica, seguida por Cry1Ab e Cry1Aa. A proteína Cry1Ca não foi tóxica às lagartas e, portanto, não foi possível determinar os seus parâmetros de toxicidade CL 50 e CL 90 . As proteínas Cry1Aa, Cry1Ab e Cry1Ac são capazes de se ligar a um mesmo receptor nas membranas intestinais, o que aumenta o risco do desenvolvimento de resistência cruzada. Portanto, a utilização conjunta dessas proteínas deve ser evitada.Termos para indexação: Bacillus thuringiensis, manejo da resistência, manejo integrado de pragas, piramidação de genes, proteínas inseticidas, vesículas de membrana. Toxicity and binding capacity of Cry1 proteins to Helicoverpa armigera (Lepidoptera: Noctuidae) intestine receptorsAbstract -The objective of this work was to evaluate the toxicity and the binding capacity of the Cry1Aa, Cry1Ab, Cry1Ac, and Cry1Ca proteins, from Bacillus thuringiensis, to Helicoverpa armigera intestine receptors. Binding analysis of the activated proteins to the brush-border membrane vesicles (BBMV) in the midgut of H. armigera, besides heterologous competition assays to evaluate their binding capacity, was performed. Cry1Ac stood out as the most toxic protein, followed by Cry1Ab and Cry1Aa. The Cry1Ca protein had no toxicity to the caterpillars and, therefore, it was not possible to evaluate its LC 50 and LC 90 toxicity parameters. The Cry1Aa, Cry1Ab, and Cry1Ac proteins are able to bind themselves to the same receptor in the midgut membrane, which increases the risk of developing cross-resistance. Therefore, the use of these proteins together should be avoided.

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Bt resistance alleles in field populations of pink bollworm from China: Similarities with the United States and decreased frequency from 2012 to 2015

Wang

Cong

et al. 2019

Pest Management Science

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BACKGROUND: Although most monitoring of pest resistance to widely cultivated transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) relies on bioassays, DNA screening for alleles associated with resistance has some advantages, particularly for rare, recessively inherited resistance. In China's Yangtze River Valley, where farmers first planted transgenic cotton producing Bt toxin Cry1Ac in 2000, bioassays have been used to monitor the recessive resistance of pink bollworm (Pectinophora gossypiella). Previous bioassay results show a small but significant increase in resistance to Cry1Ac during 2008-2010, followed by a significant decrease in resistance during 2011-2015 associated with extensive planting of second-generation hybrid cotton seeds that boosted the percentage of non-Bt cotton. Here we screened DNA from 19 748 pink bollworm collected during 2012-2015 from the Yangtze River Valley for seven alleles associated with resistance to Cry1Ac. These alleles were previously identified from lab-selected strains; three from the U.S. and four from China. RESULTS: The most common resistance allele was first identified from the U.S. and accounted for over 71% of all resistance alleles detected. Resistance was rare, with the total frequency of the seven resistance alleles showing a significant, 2.3-fold decrease from 0.0105 (95% CI: 0.0084-0.0132) in 2012 to 0.0046 (0.0031-0.0067) in 2015. CONCLUSIONS: The DNA screening data confirm results from bioassays showing pink bollworm resistance to Cry1Ac remained rare in the Yangtze River Valley from 2012-2015. The prevalence in China of the resistance allele identified from the U.S. implies a shared genetic basis of resistance that could facilitate molecular monitoring of resistance.

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DNA-based screening for an intracellular cadherin mutation conferring non-recessive Cry1Ac resistance in field populations of Helicoverpa armigera

Cited by 23 publications

References 39 publications

Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India

Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India

Toxicidade e capacidade de ligação de proteínas Cry1 a receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae)

Bt resistance alleles in field populations of pink bollworm from China: Similarities with the United States and decreased frequency from 2012 to 2015

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