DNA-Based Immunization against Lyssaviruses

Perrin, Pierre; Jacob, Yves; Tordo, Noël

doi:10.1159/000053998

Cited by 17 publications

(11 citation statements)

References 27 publications

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“…This response is related to the presence of antibodies. IL-4 detection has previously been reported by Perrin et al (25) after administration of a naked DNA rabies vaccine in dogs. Moreover, the production of IFN and high antibody titres in sheep vaccinated with a recombinant adenovirus and with an edible vaccine that express the GpVR have been demonstrated (2) .…”

Section: Discussionmentioning

confidence: 69%

“…Esta respuesta está relacionada con la presencia de anticuerpos. La detección de IL-4 fue previamente informada por Perrin et al (25) después de la administración de una vacuna de ADN desnudo contra la rabia en perros. Por otra parte se ha demostrado la producción de IFN- y altos títulos de anticuerpos en ovejas vacunadas con un adenovirus recombinante y con una vacuna comestible que expresa GpVR (2) .…”

Section: Discussionunclassified

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Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

Rojas-Anaya¹,

Esquivel-Guadarrama

Escribano-Romero

et al. 2015

Rev. Mex. Cienc. Pecu.

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RESUMENEl objetivo de este estudio fue comparar el comportamiento de IFN- e IL-4 en ratones inoculados con proteína G recombinante del virus de la rabia, que se expresa en maíz transgénico o baculovirus. Para este propósito, grupos de ratones se inocularon de la siguiente manera: Grupo 1, vacuna contra el virus de la rabia inactivado vía im; Grupo 2, proteína G recombinante derivada de plantas por vía oral; Grupo 3, proteína G expresada en baculovirus vía im; Grupo 4, proteína G expresada en baculovirus por vía oral; Grupo 5, maíz no transformado por vía oral. Los niveles de IFN- e IL-4 y los anticuerpos específicos se evaluaron cada 15 días. El desafío se realizó a los 60 días post inoculación (pi). Los grupos 1 y 3 promovieron una mejor respuesta humoral. Por otro lado, los resultados mostraron que los mismos grupos mostraron los mejores niveles de IFN- en el día 10 pi; mientras que la IL-4 se detectó en el día 15 pi. Para el estudio de supervivencia, el 83 % de los ratones inmunizados con vacuna inactivada, maíz y los inoculados im con extractos de baculovirus sobrevivieron la infección viral. La proteína G de la rabia recombinante expresada en baculovirus promovió IFN- y los anticuerpos de una manera similar a la vacuna de rabia inactivada. A pesar de esto los ratones alimentados con maíz transgénico sobrevivieron al desafío en el mismo porcentaje que el grupo 3. PALABRAS CLAVE INTRODUCCIÓNUn gran número de las vacunas contra la rabia se han desarrollado utilizando diferentes sistemas, incluyendo baculovirus y plantas (1)(2)(3)(4)(5)(6)(7) . Una de las principales ventajas del sistema de expresión de células de insecto-baculovirus es el alto rendimiento de la proteína recombinante producida por litro de cultivo celular (8) . Aunque se ha informado de la expresión de la proteína G del virus de la rabia en diferentes sistemas vegetales (7,9) el desarrollo de una vacuna oral contra la rabia reduciendo el costo de producción y distribución sería muy útil, especialmente para los países en desarrollo, donde la enfermedad es endémica en humanos y animales domésticos. La glicoproteína es el antígeno principal del virus de la rabia, es una proteína transmembrana, que consiste en un dominio citoplásmico, un dominio transmembranal y un ectodominio expuesto como trimero. El ectodominio está involucrado en la inducción de anticuerpos neutralizantes y linfocitos T citotóxicos y cooperadores (10,11) . Muchos estudios han demostrado que los extractos crudos del antígeno recombinante purificado de los extractos puros de las plantas o la planta entera pueden inducir respuestas inmunes locales o sistémicas (1,7,12) . En la literatura existen pocos estudios que han evaluado el tipo de citocinas inducidas por las vacunas antirrábicas de nueva generación (13) .El objetivo de este estudio fue comparar la respuesta de IFN- e IL-4 en ratones inoculados con proteína G recombinante del virus de la rabia (rGpRV) que se expresó tanto en células de insecto infectadas con baculovirus como en maíz transgénico. Para este propósito, la...

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Section: Discussionmentioning

confidence: 69%

Section: Discussionunclassified

Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

Rojas-Anaya¹,

Esquivel-Guadarrama

Escribano-Romero

et al. 2015

Rev. Mex. Cienc. Pecu.

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show abstract

“…Antigen expression is increased when an untranslated region, with its splicing signals, follows the promoter. 26 The sequence encoding the antigen is 3 -flanked by the transcript termination/polyadenylation (polyA) signal of the SV40 or of the bovine growth hormone (BGH). The plasmid pcDNA3 and its derivatives, which contain the HCMVie promoter and the BGH polyA, have often been used as vectors for DNA vaccines (Fig.…”

Section: Dna Vaccine An Overviewmentioning

confidence: 99%

DNA Mediated Vaccines Delivery Through Nanoparticles

Shah¹,

Ali²,

Rostami³

et al. 2015

j nanosci nanotechnol

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Vaccination has led to the eradication of those diseases which had once claimed millions of lives worldwide; however, it is accompanied with a number of dis-advantages especially safety issues until the entry of DNA vaccines. The DNA vaccines have been emerged as the best remedy for problematic diseases being capable of producing humoral and cellular immune responses as well as the safest vaccines so far. However, the magnitude of immune responses produced in primates is lower than that in experimental animals. There are several reasons described theoretically for this limited efficacy and a number of novel approaches have been applied to boost their immune responses, e.g., use of more efficient promoters and coding optimization, addition of traditional or genetic adjuvants, electroporation, intradermal delivery and various prime-boost strategies. One of these strategies is controlled antigen administration of plasmid DNA through microspheres and nanoparticles. This approach is accompanied with a number of advantages to overcome the limitations of traditional delivery systems in terms of stability, solubility and pharmacology. Furthermore, the surface structure of a virus highly resembles with a nanoparticle because of their geometrical regularities and nanoscale dimensions; therefore, the engineering of nanoparticles is based upon principles of natural virus attack which will be the best tool for vaccination. There is evidence that these immune responses can be augmented by properly structured nanosized particles (nanoparticles) that may avoid DNA degradation and facilitate targeted delivery to antigen presenting cells. Adsorption, formulation or encapsulation with particles has been found to stabilize DNA formulations. The use of nanoparticles for DNA vaccine delivery is a platform technology and has been applied for delivery of a variety of existing and potential vaccines successfully.

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“…Antigen expression is increased when an un-translated region, with its splicing signals, follows the promoter. 52 The sequence encoding the antigen is 3 -flanked by the transcript termination/polyadenylation (polyA) signal of the SV40 or of the bovine growth hormone (BGH). The plasmid pcDNA3 and its derivatives, e.g., pVAX1 (Fig.…”

Section: The Biology Of Dna Vaccine and Underlying Conceptmentioning

confidence: 99%

Nanoparticles for DNA Vaccine Delivery

Shah¹,

He²,

Li³

et al. 2014

j biomed nanotechnol

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Nanotechnology is the development of engineered devices or materials at the micro molecular level in the nanometer range. The properties of nanoparticles that these could be designed, manufactured and introduced into the human body, have led to its application in various fields of medicine. They are being used for construction of diagnostic devices, contrast agents, analytical tools, physical therapy, drug discovery and drug delivery vehicles. The DNA vaccines have been emerged as best remedy for problematic diseases being capable of producing humoral and cellular immune responses as well as the safest vaccines so far. There are a large number of infectious diseases against which traditional vaccines failed to respond effectively. Especially, viral diseases and cancer where DNA vaccines seem to be the better option. However, the magnitude of immune responses produced by them in primates is not sufficient to be used in human beings. There is an evidence that these immune responses can be augmented by using properly structured nano-sized particles that may avoid DNA degradation and facilitate targeted and controlled delivery to antigen presenting cells. Adsorption, formulation or encapsulation with particles has been found to stabilize DNA formulations. The use of nanoparticles for vaccine delivery is a platform technology and has been applied for delivery of a variety of existing and potential vaccines successfully.

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DNA-Based Immunization against Lyssaviruses

Cited by 17 publications

References 27 publications

Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

Respuesta de IFN-ɤ e IL-4 en ratones inoculados con una proteína G recombinante del virus de la rabia

DNA Mediated Vaccines Delivery Through Nanoparticles

Nanoparticles for DNA Vaccine Delivery

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