DNA-based hybridization chain reaction and biotin–streptavidin signal amplification for sensitive detection of Escherichia coli O157:H7 through ELISA

Guo, Qi; Han, Jiaojiao; Shan, Shan; Liu, Daofeng; Wu, Song-Song; Xiong, Yonghua; Lai, Weihua

doi:10.1016/j.bios.2016.07.049

Cited by 98 publications

(36 citation statements)

References 23 publications

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“…At present, E. coli detection methods mainly include the traditional culture counting method [3], molecular biological detection technology [4], immunological detection method [5], surface plasmon resonance (SPR) [6], Chemiluminescence immunoassay [7], etc. The traditional counting method for E. coli detection is based on culturing and isolation of bacteria in a specific medium, followed by observing strategy with three probes.…”

Section: Introductionmentioning

confidence: 99%

“…However, PCR technology is costly and prone to produce false negatives [9], which limits the extensive application of PCR. In addition, enzyme-linked immunosorbent assay (ELISA), as a common immunological detection technology, has also been used in bacterial test [5,10]. ELISA technology undergoes a color change by the catalysis of the enzyme in the specific recognition process.…”

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confidence: 99%

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Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

et al. 2019

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An aptamer-based electrochemical biosensor was successfully developed and applied in the rapid detection of pathogenic Escherichia coli (E. coli) in licorice extract. The thiolated capture probes were firstly immobilized on a gold electrode, and then the biotinylated aptamer probes for E. coli were introduced by hybridization with the capture probes. Due to the stronger interaction between the aptamer and the E. coli, a part of the biotinylated aptamers will dissociate from the capture probes in the presence of E. coli. The residual biotinylated aptamer probes can quantitatively bind with streptavidin-alkaline phosphatase. Subsequently, α-naphthyl phosphate substrate was catalytically hydrolyzed to generate electrochemical response, which could be recorded by a differential pulse voltammetry. The dependence of the peak current on the logarithm of E. coli concentration in the range from 5.0 × 10 2 colony forming units (CFU)/mL to 5.0 × 10 7 CFU/mL exhibited a linear trend with a detection limit of 80 CFU/mL. The relative standard deviation of 5 successive scans was 5.3%, 4.5% and 1.1% for 5.0 × 10 2 , 5.0 × 10 5 and 5.0 × 10 7 CFU/mL E. coli, respectively. In the detection of the licorice extract samples, the results obtained from the proposed strategy and traditional culture counting method were close to each other, but the time consumption was only~1/30 compared with the traditional method. These results demonstrate that the designed biosensor can be potentially utilized for rapid microbial examination in traditional Chinese medicine and relevant fields. Appl. Sci. 2019, 9, 295 2 of 15 bacterial colony morphology, color change, and biochemical reactions for quantitative detection. This detection method is economical and relatively simple to operate, but the detection time is generally 48-72 h or even longer, which cannot meet the needs for rapid detection. As an effective molecular biological detection technology, polymerase chain reaction (PCR) and the derived technology of PCR has been widely used in detecting E. coli [4,7]. PCR is based on the principle that DNA templates can denature at high temperatures, reactivate and extend (one cycle) at low temperatures by using appropriate DNA polymerase and primers. As the template DNA amplification product increases exponentially, increasing the number of cycles can magnify the specified DNA sequence by several million-fold in a short time, which greatly improves detection sensitivity [8]. However, PCR technology is costly and prone to produce false negatives [9], which limits the extensive application of PCR. In addition, enzyme-linked immunosorbent assay (ELISA), as a common immunological detection technology, has also been used in bacterial test [5,10]. ELISA technology undergoes a color change by the catalysis of the enzyme in the specific recognition process. Owing to the immune activity between antigen and antibody, ELISA method has superior specificity, high sensitivity and short detection time, and has been used in detection of different subtypes of E. col...

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

et al. 2019

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“…To overcome such limitations, polymerase chain reaction (PCR) [6] has been developed but this method used for E. coli O157:H7 detection also needs a detection time of about 24 h [7]. Several bioanalytical techniques have been developed over the last few years, like surface-enhanced raman spectroscopy (SERS) [8], flow cytometry [9], fluorescent methods [10], lateral flow immunoassay [11], hybridization chain reaction (HCR) [12], and amperometric immune sensors [13]. Among these approaches, the fluorescent technique has drawn a great deal of attention from researchers owing to its outstanding selectivity, extraordinary sensitivity, cost-effectiveness, and is non-disparaging [14].…”

Section: Introductionmentioning

confidence: 99%

Fluorimetric Detection of Single Pathogenic Bacterium in Milk and Sewage Water Using pH-Sensitive Fluorescent Carbon Dots and MALDI-TOF MS

et al. 2019

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The current study is focused on the application of water-soluble, fluorescent, and pH-sensitive carbon dots (CDs) as a nanoprobe for sensitive detection of pathogenic bacteria in milk and sewage water. The CDs were facilely synthesized through the controlled carbonization of sucrose using sulfuric acid and characterized through XRD, FTIR, TEM, UV-Vis Spectroscopy, and fluorescent analysis. The as-synthesized CDs were highly water-soluble, stable, and pH-sensitive fluorescent nanomaterials. The pH-related fluorescence study showed that the ratio of fluorescence intensity (Log[IF410/IF350]) changed linearly in the pH range between 4.9 and 6.9 in the Britton-Robison buffer. By determining the pH variation of the growth medium caused by the released acidic metabolites, the CDs-based ratiometric nanoprobe and MALDI-TOF mass spectrometry (MS) were used for the detection and identification of Escherichia coli O157:H7, respectively. The practical applicability of the pH-sensitive fluorescent CDs-based ratiometric nanoprobe was evaluated to detect Escherichia coli O157:H7 in real samples, i.e., milk and sewage water using agar count plate method with a limit of detection (LOD) up to 1 colony-forming unit per mL (CFU/mL).

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“…HCR's principle can make it possible to detect the target sensitively through signal amplification under mild conditions. In addition, the performance of HCR can be improved by fusion with signal reporter molecules, such as DNAzyme (Wang et al, ; Zhuang et al, ), Biotin receptor (Guo et al, ; Ying, Ju, Li et al, ), and Nano‐gold particle (Gao et al, ; Zou et al, ).…”

Section: Introductionmentioning

confidence: 99%

Colorimetric biosensor using dual‐amplification of enzyme‐free reaction through universal hybridization chain reaction system

Park

Rhee

Kim

et al. 2019

Biotech & Bioengineering

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On-site genetic detection needs to develop a sensitive and straightforward biosensor without special equipment, which can detect various genetic biomarkers. Hybridization chain reaction (HCR) amplifying signal isothermally could be considered as a good candidate for on-site detection. Here, we developed a novel genetic biosensor on the basis of enzyme-free dual-amplification of universal hybridization chain reaction (uHCR) and hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking

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DNA-based hybridization chain reaction and biotin–streptavidin signal amplification for sensitive detection of Escherichia coli O157:H7 through ELISA

Cited by 98 publications

References 23 publications

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

Fluorimetric Detection of Single Pathogenic Bacterium in Milk and Sewage Water Using pH-Sensitive Fluorescent Carbon Dots and MALDI-TOF MS

Colorimetric biosensor using dual‐amplification of enzyme‐free reaction through universal hybridization chain reaction system

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