DNA-Based Dynamic Mimicry of Membrane Proteins for Programming Adaptive Cellular Interactions

Jin, Li; Xun, Kanyu; Zheng, Liping; Peng, Xueyu; Qiu, Liping; Tan, Weihong

doi:10.1021/jacs.0c11245

Cited by 85 publications

(73 citation statements)

References 36 publications

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“…For instance, in 2021, Tan et al developed a cell surface imaging system based on a nanoarchitecture that mimics the behavior of membrane proteins [119]. In this study, a T-cho3 DNA nanoscaffold was designed to interact with cell membranes.…”

Section: Biosensors Based On Dna Nanostructuresmentioning

confidence: 99%

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

et al. 2022

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Several biological macromolecules adopt bivalent or multivalent interactions to perform various cellular processes. In this regard, the development of molecular constructs presenting multiple ligands in a specific manner is becoming crucial for the understanding of multivalent interactions and for the detection of target macromolecules. Nucleic acids are attractive molecules to achieve this goal because they are capable of forming various, structurally well-defined 2D or 3D nanostructures and can bear multiple ligands on their structures with precisely controlled ligand–ligand distances. Thanks to the features of nucleic acids, researchers have proposed a wide range of bivalent and multivalent binding agents that strongly bind to target biomolecules; consequently, these findings have uncovered new biosensing strategies for biomolecule detection. To date, various bivalent and multivalent interactions of nucleic acid architectures have been applied to the design of biosensors with enhanced sensitivity and target accuracy. In this review, we describe not only basic biosensor designs but also recently designed biosensors operating through the bivalent and multivalent recognition of nucleic acid scaffolds. Based on these designs, strategies to transduce bi- or multivalent interaction signals into readable signals are discussed in detail, and the future prospects and challenges of the field of multivalence-based biosensors are explored.

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Section: Biosensors Based On Dna Nanostructuresmentioning

confidence: 99%

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

et al. 2022

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“…When combining the HCR amplification with this cholesterol-DNA tetrahedral scaffold, the cell-cell interaction could also be manipulated in response to external stimuli. [24] On sensing the corresponded biological signals, the membrane-anchored DNA tetrahedral scaffolds were activated, trigging HCR amplification and forming linear DNA constructs with multiple functional modules on the cell surface. Accordingly, the attached tandem functional modules displayed multivalent effects on cell-type-specific binding or killing, providing a new approach for studying multicellular assembly and communication.…”

Section: Molecular Recognition On the Cell Membrane And Programmed Cell-cell Interactionmentioning

confidence: 99%

Regulation of Biological Functions at the Cell Interface by DNA Nanostructures

Chen

Tian

Shang

et al. 2021

Advanced NanoBiomed Research

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The control over specific biological functions of a single cell or between different cells is an exciting goal in the fields of biophysics and biochemistry. Besides the fast‐developed chemical biology methods, such as chemical labeling and gene editing, researchers are still seeking for efficient ways to manipulate cell behaviors. Over the past decades, self‐assembled DNA nanostructures have emerged as novel and versatile tools for the study of biological science. Featured with structural programmability, customized functionality, and marked biocompatibility, DNA nanostructures have been immensely exploited for a variety of fascinating biological applications including bioimaging and drug delivery. In addition, DNA nanostructures also hold great potential in the regulation of physiological functions at various biological interfaces. In this minireview, the recent progress of the regulation of biological processes and functions at cell interface using DNA nanostructures is summarized. Current challenges and opportunities in applying DNA nanoassemblies as tools for cell behavior manipulation are also briefly discussed.

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“…DNA nanotechnology has emerged as a promising tool for mimicking the functionality of proteins [1,2]. Synthetic membrane constructs have attracted particular attention, as natural ion channels [3], membrane enzymes [4], and bilayer-remodeling proteins [5] play a crucial role in every cell's survival.…”

Section: Introductionmentioning

confidence: 99%

A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels

2022

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DNA nanotechnology makes use of hydrophobically modified constructs to create synthetic membrane protein mimics. However, nucleic acid structures exhibit poor insertion efficiency, leading to a low activity of membrane-spanning DNA protein mimics. It is suggested that non-ionic surfactants improve insertion efficiency, partly by disrupting hydrophobicity-mediated clusters. Here, we employed confocal microscopy and single-molecule transmembrane current measurements to assess the effects of the non-ionic surfactant octylpolyoxyethylene (oPOE) on the clustering behavior and membrane activity of cholesterol-modified DNA nanostructures. Our findings uncover the role of aggregation in preventing bilayer interactions of hydrophobically decorated constructs, and we highlight that premixing DNA structures with the surfactant does not disrupt the cholesterol-mediated aggregates. However, we observed the surfactant’s strong insertion-facilitating effect, particularly when introduced to the sample separately from DNA. Critically, we report a highly efficient membrane-spanning DNA construct from combining a non-aggregating design with the addition of the oPOE surfactant.

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DNA-Based Dynamic Mimicry of Membrane Proteins for Programming Adaptive Cellular Interactions

Cited by 85 publications

References 36 publications

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

Regulation of Biological Functions at the Cell Interface by DNA Nanostructures

A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels

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