DNA-based detection of &lt;i&gt;Leptospira wolffii&lt;/i&gt;, &lt;i&gt;Giardia intestinalis&lt;/i&gt; and &lt;i&gt;Toxoplasma gondii&lt;/i&gt; in environmental feces of wild animals in Korea

Kumari, Priyanka; Eo, Kyung-Yeon; Lee, Woo-Shin; Kimura, Junko; Yamamoto, Naomichi

doi:10.1292/jvms.20-0596

Cited by 11 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study showed that the prevalence of G. duodenalis was relatively low compared with the results of previous studies in the ROK [25,27,[30][31][32]. Moreover, the infection rate of G. duodenalis was considerably lower than that reported globally [3,13,33,34].…”

Section: Discussioncontrasting

confidence: 58%

Multilocus genotyping of Giardia duodenalis in pre-weaned calves with diarrhea in the Republic of Korea

et al. 2023

View full text Add to dashboard Cite

Giardia duodenalis is a protozoan parasite that infects humans, companion animals, livestock, and wildlife. Infections in cattle caused by this parasite are often asymptomatic, but such infections can cause diarrhea, reduced weight gain, and ill-thrift in young calves. Although G. duodenalis causes diarrhea in calves, only a few studies have been conducted on calves in the Republic of Korea (ROK). Here, we aimed to determine the prevalence and distribution of G. duodenalis assemblages in pre-weaned calves with diarrhea in the ROK, identify the association between the occurrence of G. duodenalis and the age of calf, and perform molecular characterization of G. duodenalis. We collected 455 fecal samples from pre-weaned native Korean calves (≤60 days old) with diarrhea in four different regions. G. duodenalis was detected using nested PCR targeting the beta-giardin (bg) gene, and positive samples were further genotyped for the glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes. The overall prevalence of G. duodenalis in calves with diarrhea was 4.4% (20/455) based on the analysis of bg. The highest prevalence was observed in calves aged 11−30 days (7.5%; 95% confidence interval: 3.7%–11.3%), whereas the lowest prevalence was observed in neonatal calves. From the 20 samples that were positive for bg, 16, 5, and 6 sequences were obtained following genotyping of bg, gdh, and tpi, respectively. Sequencing analysis of the bg gene revealed the presence of assemblage E (n = 15) and sub-assemblage AⅠ (n = 1) in the samples. Moreover, we detected mixed infections with assemblages E and A in two calves for the first time. Among the sequences obtained herein, two new subtypes of assemblage E were detected in gdh and tpi sequences each. The results suggest that G. duodenalis is an infectious agent causing diarrhea in calves, and pre-weaned calves are at a higher risk of infection than neonatal calves. Multilocus genotyping should be performed to confirm the presence of potentially zoonotic genotypes. These results highlight the importance of cattle as a source of zoonotic transmission of G. duodenalis to humans.

show abstract

Section: Discussioncontrasting

confidence: 58%

Multilocus genotyping of Giardia duodenalis in pre-weaned calves with diarrhea in the Republic of Korea

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Notably, the rates of Giardia infection in wild boars have been reported to be 40.7% in Poland, 22.5% in southern Spain, 3.1% in China, 1.7% in Croatia, and 1.3% in northwestern Spain (17, 23,24,36,37). However, no studies have been (27).…”

Section: Discussionmentioning

confidence: 99%

“…Studies of Giardia infections in wild boars and domestic pigs have been conducted worldwide (17, [21][22][23][24]. However, research on Giardia in Korea has mainly focused on environmental samplesincluding drinking water, cattle-like livestock animals, and companion animals, such as dogs-and studies on wild animals are lacking (25)(26)(27)(28)(29). No studies have been conducted on Giardia infections in wild boars, and only one study is available on domestic pigs (30).…”

mentioning

confidence: 99%

Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in Korea

Lee

Kwak

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Giardia duodenalis (syn. G. intestinalis, G. lamblia) is the only Giardia species that infects humans and most other mammals. Wild boars are a reservoir of many viruses, bacteria, and parasites that can be transmitted to livestock and humans. This study examined the infection rate of G. duodenalis in wild boars and confirmed its specificity by comparing assemblages through PCR amplification of the 18S rRNA, gdh, and β-giardin genes. Fecal samples were collected from roadkilled or trapped wild boars from April 2016 to December 2021 in Korea. DNA was extracted directly from 612 wild boar fecal specimens using a commercial kit. PCR was performed targeting the 18S rRNA region, β-giardin, and glutamate dehydrogenase genes of G. duodenalis. Some PCR-positive samples were selected for sequencing analysis. The obtained sequences were subsequently used for phylogenetic tree construction. Of the 612 samples tested, 125 (20.4%) were positive for G. duodenalis. The highest infection rate was detected in the central region (12.0%) and in autumn (12.7%). Among the risk factors, the seasonal factor was statistically significant (p = 0.012). Phylogenetic analysis revealed three genetic assemblages: A, B, and E. Assemblages A and B exhibited 100% identity with Giardia sequences isolated from human and farmed pigs in Korea and Japan. This result cannot be ignored because it indicates the possibility of zoonotic transmission. Therefore, continuous management and monitoring of this pathogen are necessary to prevent transmission and protect animal and human health.

show abstract

“…We analyzed 18 fecal samples of Eurasian otters ( N = 7) and leopard cats ( N = 11). The samples in this study have been studied in our previous studies for analyses of diet [ 15 ] and zoonotic parasites [ 16 ]. The location of fecal sampling is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

The host-specific resistome in environmental feces of Eurasian otters (Lutra lutra) and leopard cats (Prionailurus bengalensis) revealed by metagenomic sequencing

Kumari

Tripathi

et al. 2022

One Health

Self Cite

View full text Add to dashboard Cite

DNA-based detection of <i>Leptospira wolffii</i>, <i>Giardia intestinalis</i> and <i>Toxoplasma gondii</i> in environmental feces of wild animals in Korea

Cited by 11 publications

References 32 publications

Multilocus genotyping of Giardia duodenalis in pre-weaned calves with diarrhea in the Republic of Korea

Multilocus genotyping of Giardia duodenalis in pre-weaned calves with diarrhea in the Republic of Korea

Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in Korea

The host-specific resistome in environmental feces of Eurasian otters (Lutra lutra) and leopard cats (Prionailurus bengalensis) revealed by metagenomic sequencing

Contact Info

Product

Resources

About