DNA Barcoding to Detect Chilli Adulteration in Traded Black Pepper Powder

Parvathy, V. A.; Swetha, V. P.; Sheeja, T. E.; Leela, N. K.; Chempakam, B.; Sasikumar, B.

doi:10.1080/08905436.2013.870078

Cited by 79 publications

(34 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The technique is currently used for detecting plant-based adulteration in many commodities including medicinal plants, commercial tea packets, olive oil, and medicinal spices (Gismondi et al, 2013;Kumar et al, 2011;Mattia et al, 2010;Stoeckle et al, 2011;Srirama et al, 2010;Vijayan & Tsou, 2010;Yuan et al, 2011). A recent study (Parvathy et al, 2014) has shown the sensitivity of this technique to detect adulteration as low as 0.5% in market samples of black pepper. Other studies have also attested the efficacy of barcoding in authenticating some socio-economically important herbs (Lockley & Bardsley, 2000;Mafra et al, 2008;Newmaster et al, 2013;Parvathy et al, 2014;Stoeckle et al, 2011;Wallace et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A recent study (Parvathy et al, 2014) has shown the sensitivity of this technique to detect adulteration as low as 0.5% in market samples of black pepper. Other studies have also attested the efficacy of barcoding in authenticating some socio-economically important herbs (Lockley & Bardsley, 2000;Mafra et al, 2008;Newmaster et al, 2013;Parvathy et al, 2014;Stoeckle et al, 2011;Wallace et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Parvathy

Swetha

Sheeja

et al. 2015

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context: In its powdered form, turmeric [Curcuma longa L. (Zingiberaceae)], a spice of medical importance, is often adulterated lowering its quality. Objective: The study sought to detect plant-based adulterants in traded turmeric powder using DNA barcoding. Materials and methods: Accessions of Curcuma longa L., Curcuma zedoaria Rosc. (Zingiberaceae), and cassava starch served as reference samples. Three barcoding loci, namely ITS, rbcL, and matK, were used for PCR amplification of the reference samples and commercial samples representing 10 different companies. PCR success rate, sequencing efficiency, occurrence of SNPs, and BLAST analysis were used to assess the potential of the barcoding loci in authenticating the traded samples of turmeric. Results: The PCR and sequencing success of the loci rbcL and ITS were found to be 100%, whereas matK showed no amplification. ITS proved to be the ideal locus because it showed greater variability than rbcL in discriminating the Curcuma species. The presence of C. zedoaria could be detected in one of the samples whereas cassava starch, wheat, barley, and rye in other two samples although the label claimed nothing other than turmeric powder in the samples. Discussion and conclusion: Unlabeled materials in turmeric powder are considered as adulterants or fillers, added to increase the bulk weight and starch content of the commodity for economic gains. These adulterants pose potential health hazards to consumers who are allergic to these plants, lowering the product's medicinal value and belying the claim that the product is gluten free. The study proved DNA barcoding as an efficient tool for testing the integrity and the authenticity of commercial products of turmeric.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of plant-based adulterants in turmeric powder using DNA barcoding

Parvathy

Swetha

Sheeja

et al. 2015

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…2 with chili (Capsicum annuum and Capsicum spp.) powder (Parvathy et al, 2014). According to Dhanya et al, (2009), papaya seeds are the most common adulterant used in black pepper seed market since both papaya and black pepper seeds have similar appearances.…”

Section: Introductionmentioning

confidence: 99%

“…According to Dhanya et al, (2009), papaya seeds are the most common adulterant used in black pepper seed market since both papaya and black pepper seeds have similar appearances. Black pepper powder adulteration with chili powder is also common because both have pungency and chili is cheaper than black pepper (Parvathy et al, 2014). However, these deceitful practices can lower the acceptability of consumers on commercially available black pepper seeds and value added products.…”

Section: Introductionmentioning

confidence: 99%

“…DNA evidences overcome the inherent fallacies associated with traditional morphometric measurements of biological entities (Dhanya and Sasikumar, 2010) and can be considered as a 100% accurate system to authenticate biological commodities. Molecular markers such as Randomly Amplified Polymorphic DNA (RAPD), Sequence Characterized Amplified Region (SCAR) (Dhanya and Shasikumar, 2010), microsatellites (Chamikara et al, 2015) and DNA barcoding methods (Parvathy et al, 2014) can be used to detect the presence of DNA from the biological adulterants. However, the employment of molecular methods to detect the biological adulterants is often hindered by the difficulty of extracting good quality DNA from dried biological samples such as papaya seeds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Length Polymorphism of the Locus psbA-trnH is Idyllic to Detect the Adulterations of Black Pepper with Papaya Seeds and Chili

et al. 2016

View full text Add to dashboard Cite

Black pepper (Piper nigrum L.) is an important spice. The adulteration of black pepper seeds and powder with papaya seeds, green chili and red chili can be seen and limited studies have been conducted to detect these adulterants. The objectives of the present study were to assess the appropriateness of morphometric methods to discriminate papaya seed and chili adulterations in black pepper and to establish a DNA based strategy to detect these adulterations. A necessary adulteration series of seeds and powders were prepared for the analyses along with commercial samples. The appearance of the seed and powder samples were slightly different but not very distinct among the pure and adulterated samples emphasizing the need of a biochemical approach to detect the adulteration. The adulterated and commercial black pepper samples received lower pungency ranks compared to that of pure samples. QIAGEN DNeasy ® Plant Mini Kit was successful in extracting PCR amplifiable DNA from any sample without papaya seeds and the modified CTAB method was able to extract required PCR amenable DNA from any sample with papaya seed material. The universal DNA barcoding primer pair, psbA-trnH, was used to amplify the DNA. Black pepper DNA yielded 200 bp band, chili and papaya DNA yielded 450 bp band and DNA from adulterated samples produced both 200 bp and 450 bp bands. Therefore this strategy can be used to detect papaya / chili adulterations in black pepper.

show abstract