DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals

Nyman, Tommi; Papadopoulou, Evangelia S.; Ylinen, Eeva; Wutke, Saskia; Michell, Craig; Sromek, Ludmila; Sinisalo, Tuula; Andrievskaya, E. R.; Alexeev, Vyacheslav; Kunnasranta, Mervi

doi:10.1016/j.ijppaw.2021.06.004

Cited by 6 publications

(4 citation statements)

References 46 publications

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Section: Discussionmentioning

confidence: 99%

“…Available evidence suggests that other species‐rich groups of ringed seal parasites exhibit parallel gradients in community diversity: many nematodes, trematodes, and cestodes found in seals in the Arctic are rare or have not been recorded in ringed seals inhabiting the Baltic Sea or lakes Saimaa and Ladoga (Delyamure et al, 1980 ; Kunnasranta et al, 2021 ; Nyman et al, 2021 ; Sinisalo et al, 2003 ). For cestodes, the low species richness in landlocked seals appears to follow from life‐cycle disruption caused by lack of intermediate or paratenic hosts (Nyman et al, 2021 ), and this might also be the case for nematodes. For example, nematodes belonging to the Contracaecum osculatum species complex that infect Arctic and Baltic seals predominantly circulate through pelagic or semi‐pelagic cod species (Gadidae) and clupeids (Clupeidae) (Johansen et al, 2010 ; Zuo et al, 2016 , 2018 ), which are absent from freshwater habitats in northern Europe.…”

Section: Discussionmentioning

confidence: 99%

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Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals

Sromek,

Ylinen,

Kunnasranta

et al. 2023

Ecology and Evolution

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Studies on host–parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple‐enzyme restriction‐site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population‐genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra‐host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals

Sromek,

Ylinen,

Kunnasranta

et al. 2023

Ecology and Evolution

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“…In contrast, parasites isolated from definitive hosts (cats and tigers) have fewer opportunities for gene exchange, thus leading to greater genetic difference. The influence of hosts on parasite genetic polymorphism is likewise limited by other conditions, such as habitat, activity range and whether the migration time of definitive hosts coincides with the period of infection [ 44 ]. Therefore, still more numerous factors need to be considered to infer the actual causes of gene exchange and genetic polymorphism of S. mansoni .…”

Section: Discussionmentioning

confidence: 99%

Genetic structure of Spirometra mansoni (Cestoda: Diphyllobothriidae) populations in China revealed by a Target SSR-seq method

Chen

Liu

et al. 2022

Parasites Vectors

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Background In China, the plerocercoid of the cestode Spirometra mansoni is the main causative agent of human and animal sparganosis. However, the population genetic structure of this parasite remains unclear. In this study, we genotyped S. mansoni isolates with the aim to improve current knowledge on the evolution and population diversity of this cestode. Methods We first screened 34 perfect simple sequence repeats (SSRs) using all available omic data and then constructed target sequencing technology (Target SSR-seq) based on the Illumina NovaSeq platform. Next, a series of STRUCTURE. clustering, principal component, analysis of molecular variance and TreeMix analyses were performed on 362 worm samples isolated from 12 different hosts in 16 geographical populations of China to identify the genetic structure. Results A total of 170 alleles were detected. The whole population could be organized and was found to be derived from the admixture of two ancestral clusters. TreeMix analysis hinted that possible gene flow occurred from Guizhou (GZ) to Sichuan (SC), SC to Jaingxi (JX), SC to Hubei (HB), GZ to Yunnan (YN) and GZ to Jiangsu (JS). Both neighbor-joining clustering and principal coordinate analysis showed that isolates from intermediate hosts tend to cluster together, while parasites from definitive hosts revealed greater genetic differences. Generally, a S. mansoni population was observed to harbor high genetic diversity, moderate genetic differentiation and a little genetic exchange among geographical populations. Conclusions A Target SSR-seq genotyping method was successfully developed, and an in-depth view of genetic diversity and genetic relationship will have important implications for the prevention and control of sparganosis. Graphical Abstract

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“…For the polymerase chain reaction, 20 ng of genomic DNA was employed (PCR). Primers for the cytochrome c oxidase 1 (COI) gene region (Table 1) were developed for PCR amplification (34). Cycling conditions (Initial denaturation 95 o C, 5 min (One cycle), denaturation 95 o C, 30 sec, annealing 60 o C, 35 sec, extension 72 o C 35 sec (39 cycle), final extension 72 o C, 5 min (One cycle).…”

Section: Molecular Analysismentioning

confidence: 99%

Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq

Alanad¹,

Farhood²

2023

IJVS

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Cestodes or tapeworms (Class Cestoda, phylum Platyhelminthes) are widespread parasites of birds, as they cause various damages, which may lead to the death of their hosts, especially when the infection is severe. The Black Francolin belongs to the Phasianidae family of pheasants and, in the order Galliformes, the gallinaceous birds, and it is called Francolinus francolinus. Considering it as a local bird in Asia, it was referred to as the black partridge. The current study was conducted from January to October 2019 to determine the cestoda infections within F. francolinus. Thirty-five birds from F. francolinus were examined for the presence of cestoda, of which 17 birds (48.57%) were infected with cestoda parasites. Molecular diagnosis based on sequences of cytochrome c oxidase 1 (COI) gene regions was performed to confirm the diagnosis of this cestoda. This revealed a close identification of up to 93.44-100% for COI gene regions with other cestoda species obtained from GenBank. Phylogenetic analysis supported the placement of this species within three families, Davaineidae, Taeniidae, and Hymenolepididae, with close relationships to the previously described species of Raillietina sp., R. mahnerti, R. hymenolepidoides, Ophryocotyloides dasi, Rodentolepis nana and Hydatigera parva based on the COI gene regions. This is the first study in Iraq to diagnose Cestoda in birds by molecular methods, where five species of cestoda parasitic on F. francolinus were recorded for the first time in Iraq.

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DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals

Cited by 6 publications

References 46 publications

Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals

Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals

Genetic structure of Spirometra mansoni (Cestoda: Diphyllobothriidae) populations in China revealed by a Target SSR-seq method

Molecular detection of cestoda in black francolin (Francolinus francolinus) of Babylon Province, Iraq

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