DNA barcoding reveals commercial fraud related to yak jerky sold in China

Wang, Yunyu; He, Kai; Fan, Lu; Wang, Yushui; Wu, Shi-Fang; Murphy, Robert W.; Wang, Wenzhi; Zhang, Yaping

doi:10.1007/s11427-015-4979-0

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…For the past 17 years, DNA barcoding has drawn great attention as a powerful species identification tool. DNA barcoding is an emerging technology for reliable and concise species identification in the food and dietary supplement industry to determine the authenticity of mushrooms, traditional Chinese medicine, herbal dietary supplements, tea, meat, yak jerky, and aquatic products such as fish, crustaceans, and mollusks (Stoeckle et al, 2011;Little and Jeanson, 2013;Shen et al, 2016;Wang et al, 2016;Raja et al, 2017;Yacoub and Sadek, 2017;Hou et al, 2018). DNA barcoding authentication has considerable merits, one of which is that recognition requires only a tiny piece of tissue, is not affected by damaged and incomplete tissue, such as in powdered mycelium samples (Raja et al, 2017), and does not have growth stage limitations.…”

Section: Discussionmentioning

confidence: 99%

DNA Barcoding Mushroom Spawn Using EF-1α Barcodes: A Case Study in Oyster Mushrooms (Pleurotus)

Zhao

Cheng

et al. 2021

Front. Microbiol.

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Oyster mushrooms (genus Pleurotus) are widespread and comprise the most commonly cultivated edible mushrooms in the world. Species identification of oyster mushroom spawn based on cultural, morphological, and cultivated characteristics is time consuming and can be extraordinarily difficult, which has impeded mushroom breeding and caused economic loss for mushroom growers. To explore a precise and concise approach for species identification, the nuclear ribosomal internal transcribed spacer (ITS), 28S rDNA, and the widely used protein-coding marker translation elongation factor 1α (EF-1α) gene were evaluated as candidate DNA barcode markers to investigate their feasibility in identifying 13 oyster mushroom species. A total of 160 sequences of the candidate loci were analyzed. Intra- and interspecific divergences and the ease of nucleotide sequence acquisition were the criteria used to evaluate the candidate genes. EF-1α showed the best intra- and interspecific variation among the candidate markers and discriminated 84.6% of the species tested, only being unable to distinguish two closely related species Pleurotus citrinopileatus and Pleurotus cornucopiae. Furthermore, EF-1α was more likely to be acquired than ITS or 28S rDNA, with an 84% success rate of PCR amplification and sequencing. For ITS and 28S rDNA, the intraspecific differences of several species were distinctly larger than the interspecific differences, and the species identification efficiency of the two candidate markers was worse (61.5 and 46.2%, respectively). In addition, these markers had some sequencing problems, with 55 and 76% success rates of sequencing, respectively. Hence, we propose EF-1α as a possible DNA barcode marker for oyster mushroom spawn.

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Section: Discussionmentioning

confidence: 99%

DNA Barcoding Mushroom Spawn Using EF-1α Barcodes: A Case Study in Oyster Mushrooms (Pleurotus)

Zhao

Cheng

et al. 2021

Front. Microbiol.

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“…The Bos indicus type (n=34) were then analyzed for the presence of different haplogroups in the presence of reference sequences for haplogroup I1 and haplogroup I2 ( Lai et al, 2006, Wang et al, 2016 both haplogroups for Bos indicus type, I1 and I2, were found in this population (Figure 2). Among them, haplogroup I1 was the predominant (64.7%, n= 22) group followed by I2 (35.3%, n= 12).…”

Section: Variation In the Maternal Lineagementioning

confidence: 99%

Mitochondrial DNA polymorphisms in Nepalese Achhami cattle

Gorkhali

Dhakal

Sapkota

et al. 2020

Bang. J. Anim. Sci.

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Achhami cattle is claimed to be the world's smallest cattle which is found in Achham district of Sudur Paschim Province of Nepal. A study was carried out to investigate the polymorphism in the control region of the mitochondrial DNA polymorphism in these Achhami cattle. Thirty-seven blood samples were collected from different pocket areas of in Achham district. Our study revealed that Achhami cattle lie significantly within the indicine haplogroups rather than taurine (34 out of 37 samples) manifesting the later introgression by taurine cattle population. The taurine haplogroup, found within Achhami was different than Lulu cattle, which might be from the independent domestication event. Within indicine haplogroup, I1 type haplogroup (64.7%) was found dominant over I2 type haplogroup (35.3%). Achhami cattle revealed its uniqueness as it segregates from Indian cattle for indicine type as well as Chinese cattle for taurine type. In order to understand its ancestry, the whole genome should be studied together with the consideration of more population of cattle from the Asian region. Bang. J. Anim. Sci. 2020. 49 (1): 22-28

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“…Discovering the characteristics of wind speed frequency distribution in wind farms is the key to the research of wind energy resources. The wind speed frequency distribution refers to the probability density function of wind speed, which describes the complete statistical properties of wind speeds displaying random behaviour [6].…”

Section: Introductionmentioning

confidence: 99%

Frequency Distribution Model of Wind Speed Based on the Exponential Polynomial for Wind Farms

2019

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This study introduces and analyses existing models of wind speed frequency distribution in wind farms, such as the Weibull distribution model, the Rayleigh distribution model, and the lognormal distribution model. Inspired by the shortcomings of these models, we propose a distribution model based on an exponential polynomial, which can describe the actual wind speed frequency distribution. The fitting error of other common distribution models is too large at zero or low wind speeds. The proposed model can solve this problem. The exponential polynomial distribution model can fit multimodal distribution wind speed data as well as unimodal distribution wind speed data. We used the linear-least-squares method to acquire the parameters for the distribution model. Finally, we carried out contrast simulation experiments to validate the effectiveness and advantages of the proposed distribution model.

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DNA barcoding reveals commercial fraud related to yak jerky sold in China

Cited by 10 publications

References 10 publications

DNA Barcoding Mushroom Spawn Using EF-1α Barcodes: A Case Study in Oyster Mushrooms (Pleurotus)

DNA Barcoding Mushroom Spawn Using EF-1α Barcodes: A Case Study in Oyster Mushrooms (Pleurotus)

Mitochondrial DNA polymorphisms in Nepalese Achhami cattle

Frequency Distribution Model of Wind Speed Based on the Exponential Polynomial for Wind Farms

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