DNA Barcoding on Bacteria: A Review

Abstract

Bacteria are omnipotent and they can be found everywhere. The study of bacterial pathogens has been happening from olden days to prevent epidemics, food spoilage, losses in agricultural production, and loss of lives. Modern techniques in DNA based species identification are considered. So, there is a need to acquire simple and quick identification technique. Hence, this review article covers the efficacy of DNA barcoding of bacteria. Routine DNA barcoding involves the production of PCR amplicons from particular regions to sequence them and these sequence data are used to identify or “barcode” that organism to make a distinction from other species.

A multiproxy approach to evaluate biocidal treatments on biodeteriorated majolica glazed tiles

“…Total DNA was extracted using the FastDNAV R SPIN for Soil Kit (MP Biomedicals, France) in conjunction with the FastPrepV R instrument, following the manufacturer's protocol. Standard DNA barcodes were used for identification (Hajibabaei et al, 2007;Schoch et al, 2012;Gismondi et al, 2013;Lebonah et al, 2014). PCR amplifications of cyanobacterial 16S rRNA gene fragments were performed using the primer pair Cya106F (5'-CGGACGGGTGAGTAACGCGTG A-3') and Cya781R (5'-GACTACTGGGGTATCTAATCCCWTT-3') as described by N€ ubel et al (1997).…”

Application of Molecular Methods for Traceability of Foodborne Pathogens in Food Safety Systems

“…As described by Lebonah et al (2014), this essentially involves identifying a short DNA sequence from a standard section of the bacterial genome and using this as a reference "barcode" for comparing unknown isolates against a reference catalogue of known barcode sequences of previously identified organisms (Hebert et al, 2003;Savolainen et al, 2005;Hajibabaei et al, 2007;Lebonah et al, 2014). As described by Lebonah et al (2014), this essentially involves identifying a short DNA sequence from a standard section of the bacterial genome and using this as a reference "barcode" for comparing unknown isolates against a reference catalogue of known barcode sequences of previously identified organisms (Hebert et al, 2003;Savolainen et al, 2005;Hajibabaei et al, 2007;Lebonah et al, 2014).…”

Sopas de diversidad para el análisis y monitoreo de artropofauna en ambientes antropizados

“…Con el fin de hacer más efectivo el análisis molecular y homogeneizar los métodos y calidad de datos surge el proyecto dna barcoding (http://www.barcodeoflife.org/), con el objetivo de estandarizar protocolos internacionales para identificar la mayor cantidad de especies del mundo. En el caso de las que pertenecen al reino animal se emplea como marcador molecular el citocromo c oxidasa i, para las plantas se utilizan matK, rbcL, rpoC1, rpoB4R, atpFatpH, psbKpsbI, trnHpsbA, para los hongos se ocupan principalmente segmentos its (Ratnashingam y Hebert, 2007;cbol Plant Working Group, 2009;Seifert, 2009) y para bacterias se emplean 16S, los grupos de rrna-i, -vii, -ix, -xii, -xv y -xx (Lebonah et al, 2014) . La secuencia obtenida del individuo colectado se ingresa a la base de datos que fue específicamente creada para este proyecto, bold Systems (http://www.barcodinglife.com/) que además de contener la información genética se complementa con la información del espécimen (colección de procedencia, datos de colecta, fotografías, mapa con puntos de colecta) y el protocolo de laboratorio.…”