DNA Barcoding of Medicinal Plants

Identification of Cumin (Cuminum cyminum) MicroRNAs through Deep Sequencing and Their Impact on Plant Secondary Metabolism

“…It suggests these cumin miRNAs can target a key regulator enzyme in both sterol and triterpenoid production due to squalene cyclization being a crucial branch point in their biosynthesis pathway [30]. Our results coincide with the findings of Srivastava et al (2018), who reported that miR5140 and miR159 families were involved in the gene regulation of cycloartenol synthase and sterol delta-7 reductase 1, which are directly involved in the biosynthesis of withanolides, a group of steroids produced by the medicinal plant Withania somnifera [17]. Similarly, Jeena et al (2021) identified the cleavage site of cycloartenol synthase by Bm-miR172c-5p from the medicinal plant Bacopa monnieri and suggested that this miRNA could be a key regulator in the plant response to abiotic stress [31].…”

“…Intriguingly, some of the previous research has also shown that a number of microRNAs are involved in secondary plant metabolism [9,14]. For instance, Barozai and colleagues (2013) identified 17 novel miRNAs in carrots, out of which five were involved in plant metabolism [16], while microRNAs miR5140, miR159, miR172, miR5303, miR5658, miR477, miR1426, miR530, and miR5079 from Withania somnifera (Indian ginseng) were found to be associated with the biosynthesis of steroid, stilbenoid, diarylheptanoid, gingerol, phenylpropanoid, isoquinoline, glycosyltransferases, ubiquinone, carotenoid, and other diterpenoids [17]. Similarly, miR4995, miR5021, and miR6300 from the medicinal plant Cinnamomum camphora are linked to the regulation of the biosynthesis of highly valuable terpenoids [18].…”

Genotype classification and fluorescence visual identification of the Rhizoma Paridis of Paris polyphylla var. yunnanensis based on the SNPs of ITS sequence 

“…It is widely used in the identi cation of authentic Chinese medicines, the identi cation of genuine products and substitutes, the identi cation of origin, the identi cation of multiorigin sources and genetic diversity, the identi cation of age, and so on. It plays an important role in ensuring the safety and effectiveness of traditional Chinese medicine, protecting the genetic diversity of medicinal plants, and nding or expanding new drug sources (Cai et al 2017;Srivastava et al 2016). Among them, ITS (ITS1/ITS2) sequence was recommended to be included in the core barcode of seed plants by China Plant Barcode Of Life Group (China Plant BOL Group) in 2011.…”