DNA Barcoding of genus Culicoides biting midges (Diptera: Ceratopogonidae) in the Brazilian Amazon

Carvalho, Luís Paulo Costa; Costa, Glaucilene da Silva; Júnior, Antônio Marques Pereira; Paulo, Paula Frassinetti Medeiros de; Silva, Geisiane Santos; Carioca, Angélica Lorena Pereira Mendes; Rodrigues, Bruno Leite; Pessoa, Felipe Arley Costa; Medeiros, Jansen Fernandes

doi:10.1016/j.actatropica.2022.106619

Cited by 4 publications

(3 citation statements)

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“…An argument supporting that hypothesis in our sample is that, despite of significant WGM between geographic locations, there was molecular divergence between populations ( Figure 3 ). Significantly, based on Cox1, the barcode gap between populations from Moselle and Corsica/Var is >2.5%; generally, a 2% gap is used to separate one species from the next ( 72 ). In contrast, Corsica and Var populations are grouped in different branch in our tree ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…Few studies have investigated Culicoides sequences of Cox1 in several areas ( 14 , 15 , 74 , 75 ). The intraspecific mtDNA genetic distances could vary considerably among species ( 72 , 76–80 ) and it is difficult to give a DNA barcode distance threshold to species delimitation. Nevertheless, a high value of intraspecific distance could be indicative of the early stages of speciation.…”

Section: Discussionmentioning

confidence: 99%

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Wing morphology variations in Culicoides circumscriptus from France

Hadj-Henni

Djerada²,

Millot

et al. 2023

Front. Vet. Sci.

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The biting midge Culicoides circumscriptus Kieffer, 1918 is a European widespread vector of avian malaria throughout the continent and is a possible vector of Akabane virus and Bluetongue virus. This species populates a wide range of environments in contrasting ecological settings often exposed to strong seasonal fluctuations. The main goals of this study were to investigate C. circumscriptus phenotypic variation at three departments in France (Corsica Island, Moselle and Var) and to determine if its phenotypes vary with the environment. Culicoides circumscriptus wing phenotypes were analyzed using a geometric morphometric approach based on anatomical landmarks and outlines of the wing. Dendogram trees based on landmarks and the outlines-2 set (cell m4) showed similar topologies and separated populations of C. circumscriptus. In contrast, another set of outlines-1 (covering the r-m cross vein, M, radiale and arculus) presented a different hierarchical clustering tree. The phenotypic variation observed in C. circumscriptus indicated that these populations are exposed to environmental and ecological pressures. Our results suggest the presence of phenotypic plasticity in this species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Wing morphology variations in Culicoides circumscriptus from France

Hadj-Henni

Djerada²,

Millot

et al. 2023

Front. Vet. Sci.

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“…The COI marker is an effective tool for unambiguously identifying different species of Culicoides . It shows a pattern of low intraspecific and high interspecific variation, even when cryptic species are present, being able to distinguish species even with a low grade of morphological differences (Pagès et al 2009, Ander et al 2013, Gopurenko et al 2015, Nielsen and Kristensen 2015, Carvalho et al 2022). However, COI-based mitochondrial sequence analyses may not be sufficient to explore the phylogenetic relationships between closely related or disputed taxa.…”

Section: Discussionmentioning

confidence: 99%

Shedding light on the controversial taxonomic status of Culicoides jamaicensis and Culicoides paolae (Diptera: Ceratopogonidae): an overseas trip among continents

Bravo‐Barriga

González

Parreira

et al. 2023

Journal of Medical Entomology

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Culicoides biting midges (Diptera: Ceratopogonidae) are small bloodsucking flies that act as vectors for various pathogens of medical and veterinary importance. This study aimed to examine, using a comprehensive approach, the controversial taxonomic status of 2 Culicoides species that are currently distributed in the Neotropical (Culicoides jamaicensis Edwards) and Palearctic (Culicoides paolae Boorman) areas and possess unique and distinctive features. Previous investigations based on morphological analysis have suggested that these 2 species may be synonyms. Our work updated the current geographical distribution of both species and analyzed new specimens from different geographic origins, together with publicly available sequences. We used 2 universal genetic markers (COI and 28S) to test this hypothesis. Our study reveals evidence that C. paolae and C. jamaicensis belong to the same species due to the following statements: (i) similar morphological features; (ii) low interspecific genetic variation; (iii) association with a single genetic cluster; (iv) inclusion within the subgenus Drymodesmyia, which has only been recorded in the New World; and (v) occurrence in habitats with moderate temperatures. We recommend that European and African specimens of C. paolae be considered from now on as C. jamaicensis. Our comprehensive approach shed new light on the taxonomic status of these 2 Culicoides species and has implications for future studies on their biology and ecology.

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