DNA barcoding of Deltocephalus Burmeister leafhoppers (Cicadellidae, Deltocephalinae, Deltocephalini) in China

Abstract

We investigated the feasibility of using the DNA barcode region in identifying Deltocephalus from China. Sequences of the barcode region of the mitochondrial COI gene were obtained for 98 specimens (Deltocephalusvulgaris – 88, Deltocephaluspulicaris – 5, Deltocephalusuncinatus – 5). The average genetic distances among morphological and geographical groups of D.vulgaris ranged from 0.9% to 6.3% and among the three species of Deltocephalus ranged from 16.4% to 21.9% without overlap, which effectively reveals the existence of a “DNA barcoding gap”. It is important to assess the status of these morphological variants and explore the genetic variation among Chinese populations of D.vulgaris because the status of this species has led to taxonomic confusion because specimens representing two distinct morphological variants based on the form of the aedeagus are often encountered at a single locality. Forty-five haplotypes (D.vulgaris – 36, D.pulicaris – 5, D.uncinatus – 4) were defined to perform the phylogenetic analyses; they revealed no distinct lineages corresponding either to the two morphotypes of D.vulgaris or to geographical populations. Thus, there is no evidence that these variants represent genetically distinct species.

The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae

“…The genetic distance reflects the distance of the genetic relationships between each tribe. Among the four tribes, Typhlocybini and Zyginellini have the smallest genetic distance, indicating that the relationship between the two is the closest, which is consistent with the results of morphological studies ( Zhang 1990 ; Huang 2013).…”

“… Jiao (2017) used MP and NJ methods to analyze the phylogenetic relationship between Alebrini and Dikraneurini based only on morphological data. The results showed that the two tribes’ monophyly was well supported, and its position in the evolutionary tree was similar to that of Zhang (1990) and showed that Alebrini is more primitive.…”

“…8E ). Dworakowska (1977 , 1990) discussed Typhlocybini with respect to other tribes, and postulated that the different connection modes of the hindwing peripheral vein and CuA represented different branches, and divided Zyginellini from Typhlocybini resulting in six tribes: Alebrini , Dikraneurini , Empoascini , Erythroneurini , Typhlocybini , and Zyginellin, while Zhang (1990) postulated that Erythroneurini evolved from Empoascini . Since then, Typhlocybinae -related research has followed Dworakowska’s six-tribe classification system (Fig.…”

Characterization of Two Complete Mitochondrial Genomes of Ledrinae (Hemiptera: Cicadellidae) and Phylogenetic Analysis

“…The mitochondrial gene cox1 is one of the commonly used barcodes for identifying species and inferring the phylogenetic relationship in leafhoppers [ 67 , 68 , 69 ], but it was the slowest evolving among the PCGs in these four Ledrinae species, while atp8 was a comparatively faster evolving gene. In this study, we found nad6 and atp8 could be evaluated as potential DNA markers for sibling species delimitation.…”

DNA barcodes for delineating Clerodendrum species of North East India

“…For ML analysis, the phylogenetic trees were constructed in RAXMLGUI v1.3.1, a graphical front-end for RAXML 45 . The clade support was assessed using ML with thorough bootstrap analyses, run 10 times starting from random seeds under GTRGAMMA model and 1,000 non-parametric bootstrap values 46 . The species forming separate clusters in the tree with bootstrap support > 50% were considered to be distinct.…”