DNA barcoding of arid wild plants using rbcL gene sequences

Abstract

ABSTRACT. DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We therefore tested the potential of the rbcL marker for the identification of wild plants belonging to diverse families of arid regions. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identification of the majority of the samples (92%) to genus level and only 17% to species level.

Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

“…Universal bar code should possess three characteristics, short sequence, universality, and unique identifiers (Stoeckle 2003), which was more applicable to algae than to land plants due to the absence of variable identifiers (Presting 2006). So far, single barcode region suitable to all plant taxa is not reported (Ballardini et al 2013); different barcodes are successful to different taxa, for example, rbcL locus for palmae (Naeem et al 2014;Bafeel et al 2012); ITS for Asteraceae (Gong et al 2015); multiple loci, ITS ? rpoB and rpoC1 for Resedaceae ; rbcL ?…”

Evaluating the utility of rbcL in identifying Nigerian speciesof the genus Afzelia Sm. (Fabaceae; Caesalpinioideae)

“…Based on the intraspecific and interspecific divergence, our results revealed between 0.000 and 0.001 intraspecific distance among species and their conspecifics and a mean interspecific distance of 0.002 ranging from 0.001 to 0.006; this depicts an overlap between intra-and interspecific divergence. Although the rbcL region has been used successfully in some studies (Meier et al, 2008;Bafeel et al, 2011Bafeel et al, , 2012Dong et al, 2014) to accurately identify species, this region could not accurately identify our understudied species. This could be due to the low variability of the rbcL region (Gao et al, 2010).…”

DNA Fingerprinting Profile and Quality Control Standardization of Folklore Medicinal Plant Exacum lawii

“…15,16 Sequences of the primers (Sigma grade) used for RAPD-PCR are given in Table 1. PCR was performed in 25 μl reaction mixture containing 40 ng DNA, 2.5 μl of 10X PCR buffer (500 μM dNTPs, 4.5 mM MgCl 2 , 0.5µM of each primer and 0.6 unit per µl Taq DNA polymerase (Bangalore Genei, India) in an Icycler PCR thermal cycler (Bio-Rad, USA).…”