DNA Barcoding of Andaliman (Zanthoxylum acanthopodium DC) from North Sumatra Province of Indonesia using maturase K Gene

Suriani, Cicik; Prasetya, Eko; Harsono, Tri; Manurung, Johannes; Prakasa, Hary; Handayani, Dina; Jannah, Miftahul; Rachmawati, Yuanita

doi:10.21315/tlsr2021.32.2.2

Cited by 6 publications

(1 citation statement)

References 23 publications

(30 reference statements)

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“…It has higher mutations and undergoes more rapid evolution compared to rbcL. The matK also has the ability to differentiate several species in Angiosperms (Patwardhan et al, 2014) such as Sangihe Nutmeg (Myristica fragrans) (Tallei & Kolondam, 2015), flowering plants in Sumatra (Amandita et al, 2019), Metroxylon sagu (Abbas et al, 2020), andaliman (Zanthoxylum acanthopodium DC) from North Sumatra (Suriani et al, 2021), and some rare fruits in West Kalimantan (Candramila et al, 2023). Meanwhile, the trnL-trnL-trnF IGS sequence includes the intron region of the trnL(UAA) gene and the spacer between the trnL(UAA) and trnF(GAA) genes.…”

Section: Introductionmentioning

confidence: 99%

Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer

Roslim,

Herman,

Putri

et al. 2023

J Bio Bio Edu

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Maman is a traditional plant frequently used as food by people in Melayu Rokan of Riau Province. Morphological detection indicates that the scientific name of maman is Cleome gynandra L. The objective of this study is to confirm the taxonomic status of maman from Riau basedon matK and trnL-trnL-trnF intergenic spacer (IGS) sequences. Methods utilized in this study included sampling, DNA extraction, PCR, electrophoresis, sequencing, and data analysis. The fresh leaves were picked up from Rokan Hulu Regency, Riau Province. In this study, the matK and trnL-trnL-trnF IGS sequences of maman with a length of 754 bp and 938 bp, respectively, had been obtained. The BLASTn analysis based on both sequences showed that maman had 100% similarity to Gynandropsis gynandra. Cleome gynandra was synonymous with G. gynandra. There were 49 nucleotide variations, 16 critical nucleotides, and there were no indels in matK. Meanwhile, in trnL-trnL-trnF IGS there were 181 nucleotide variations, 13 critical nucleotides, and 61 indels. In conclusion, this study succeeded in confirming the taxonomic status of maman from Riau as C. gynandra syn. G. gynandra. The result enriched the abundance of DNA barcode sequences of this plant in GenBank which is useful for the molecular identification.

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Section: Introductionmentioning

confidence: 99%

Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer

Roslim,

Herman,

Putri

et al. 2023

J Bio Bio Edu

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Farm business analysis and marketing channels for Andaliman agroforestry in Humbang Hasundutan District

Pane

Rumaijuk

Jufri

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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As an endemic plant from the vicinity forest around Lake Toba area in North Sumatera Province, Andaliman (Zanthoxylum acanthopodium DC) is very beneficial. However, its utilization depends on Batak communities’ traditions so that it is not cultivated and marketed extensively. Due to the uncertain price at the farmers level which had become an obstacle in marketing Andaliman, this study was conducted to provide data and suggestions about farm business analysis and marketing channels for Andaliman agroforestry in Humbang Hasundutan District, hoping it can assist in formulating policies related to price stabilization. The study results show that the farmers’ income was still very varied between IDR 2,441.03-73,882.37 with an average IDR 31,080.32. The Andaliman’s marketing channels in Humbang Hasundutan District consisted of three channels, where Channel I was the longest and most common one, with four marketing agencies involved, namely the farmers, the village level middlemen, the district level middlemen, and the retailers.

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Genetic diversity and relationship of mango and its wild relatives (Mangifera spp.) based on morphological and molecular markers

Mursyidin

2023

Pesqui. Agropecu. Trop.

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Mango and its wild relatives (Mangifera spp.) are essential for future mango breeding, including preservation programs, because they provide many beneficial genes (agronomic traits), particularly those related to resistance to biotic and abiotic stressors. However, there is a limited understanding of the genetic diversity and relationships of this germplasm. This study aimed to determine the diversity and relationship between endemic mango and its wild relatives (Mangifera spp.) from Borneo Island, Indonesia, using leaf morphology and the internal transcribed spacer (ITS) region. Fifteen samples of Mangifera, covering 12 species, were used. Morphologically, the endemic Mangifera had a low diversity of only 0.22. Based on the ITS sequence, Mangifera endemic to Borneo had a high level of genetic diversity (0.069). In addition, this sequence had a total variable number of 215 bp, of which 110 bp were singleton sites, 89 informative parsimony and 41 indels. Phylogenetic analysis showed that Mangifera was grouped into three clusters for leaf morphological traits and four clades for the ITS region. In this case, the furthest relationship was pointed out by ‘Hampalam’ (M. laurina) and ‘Tambusui’ (M. macrocarpa), as well as by ‘Rawa-Rawa’ (M. similis) and ‘Samputar’ (M. torquenda). In contrast, the closest relationship was shown by ‘Hambawang Damar’ (M. foetida) and ‘Hambawang Puntara’ (M. foetida), including ‘Samputar’ (M. torquenda) and ‘Pauh’ (M. quadrifida). In particular, the common mango (M. indica) was closely related to ‘Asam Buluh’ and ‘Hampalam’ (M. laurina) and distantly related to ‘Pauh’ (M. quadrifida) and ‘Rawa-Rawa’ (M. similis).

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DNA Barcoding of Andaliman (Zanthoxylum acanthopodium DC) from North Sumatra Province of Indonesia using maturase K Gene

Cited by 6 publications

References 23 publications

Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer

Verification of Maman (Cleome gynandra (L.) Briq.) from Riau Based on matK and trnL-trnL-trnF Intergenic Spacer

Farm business analysis and marketing channels for Andaliman agroforestry in Humbang Hasundutan District

Genetic diversity and relationship of mango and its wild relatives (Mangifera spp.) based on morphological and molecular markers

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