DNA barcoding is an effective tool for differentiating &lt;I&gt;Pisolithus&lt;/I&gt; species from Macedonia

Rusevska, Katerina; Karadelev, Mitko; Phosri, Cherdchai; Dueñas, Margarita; Tellería, M. Teresa; Watling, Roy; Martı́n, Marı́a P.

doi:10.5248/130.1007

Cited by 6 publications

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“…In the genus Tulostoma, as in other gasteroid fungi (Kruger et al, 2001;Martín et al, 2013;Phosri et al, 2013;Rusevska et al, 2015), the barcoding region is very useful to discriminate species, mainly when the morphological features are very similar, such as a not clearly defined mouth, difficulty in defining the structure of the exoperidium, or indistinguishable spore ornamentation even under SEM. In general, such as in Jeppson et al (2017), terminal clades are highly supported for T. fimbriatum, T. wintherhoffii, or the clade of T. melanocyclum, T. squamosum, and T. subsquamosum.…”

Section: Discussionmentioning

confidence: 99%

Fungal DNA barcode (ITS nrDNA) reveals more diversity than expected in Tulostoma from Macedonia

Rusevska¹,

Calonge²,

Karadelev³

et al. 2019

Turk J Bot

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The genus Tulostoma Pers. (Order Agaricales; Basidiomycota) is characterized by a globose spore-sac, where the spores are formed, attached onto a stipe; they are known commonly as stalked puffballs. The sporesac is formed by the peridium that envelopes the gleba; when spores are mature, they are released via an opening found on the apical part of the sac called a mouth or stoma that can be tubular, circular, or elliptical; fibrillose or fimbriate; or a simple crevice. The current genus was initially divided into two sections: Eutylostoma and Schizostoma (Fischer, 1900, 1933; Petri, 1909; Fries, 1921), which are differentiated by mouth morphology. Pouzar (1958) offered a more precise classification based on the morphology and mode of opening of the exoperidium, as well as the morphology of the mouth and stipe, proposing four sections: Brumalia, with tubular mouth; Poculata, with mouth fibrillose; Fimbriata, with fimbriate mouth; and Volvulata, with irregular mouth. Wright (1987) supplemented Pouzar's classification with primary and secondary micromorphological characteristics. The primary characteristics are the endoperidium form, size, and color; exoperidium color, persistence, and decay; mouth form; and spore size and ornamentation. Secondary characteristics are stipe size, and color and morphology of stipe surface; thickness of capillitium hyphae; and morphology of capillitium transverse septa. In Wright's classification two subgenera are included: Tulostoma Pers. and Lacerostoma J. E. Wright, encompassing 137 species worldwide. As pointed out by Cunningham (1925), Moreno et al. (1992) considered that this genus consists of a taxonomic complex, since some characters are not always easy to differentiate. The genus has been studied in various areas of Europe, Asia Minor, and Africa. In the work of Kreisel (2001), 28 species were cited in Europe, 14 in Asia Minor, and 38 in Africa. In the Mediterranean area, Calonge et al. (2007) described 22 species for Spain, while Tkalčec et al. (2005) and Sesli and Denchev (2008) reported only five species in Croatia and Turkey. In Macedonia, based on morphological identification, Karadelev and Rusevska (2009) confirmed four species: Tulostoma brumale Pers., T. fimbriatum Fr., T. melanocyclum Bres., and T. squamosum (J.F. Gmel) Pers. (Table). Wright (1987) reported, for the first and only time, T. caespitosum Trab. Molecular analysis based on the internal transcribed spacers (ITS) of nuclear ribosomal DNA (nrDNA), accepted as the DNA barcode for fungi (Schoch et al., 2012), provides valuable information for species identification. Recently, Jeppson et al. (2017) demonstrated that the ITS region is very useful to discriminate Tulostoma species in Europe; in this paper the barcode sequence was obtained Abstract: With the aim of clarifying the number of Tulostoma species in Macedonia, and to verify some previous collections, molecular analyses were carried out on four morphologically identified species collected in different habitats (at the edges of oak or juniper forests, pine ...

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Section: Discussionmentioning

confidence: 99%

Fungal DNA barcode (ITS nrDNA) reveals more diversity than expected in Tulostoma from Macedonia

Rusevska¹,

Calonge²,

Karadelev³

et al. 2019

Turk J Bot

Self Cite

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“…It was introduced by Albertini and Schweinitz (1805) to accommodate the type species Pisolithus arenarius. Members of this genus were recorded from various habitats with regard to temperature, nutrient levels and heavy metal concentrations, and has been recorded from tropical, subtropical and temperate regions (Watling et al 1999;Martín et al 2013;Rusevska et al 2015;Jaouani et al 2015;Crous et al 2016;Mifsud & Mifsud 2022). Pisolithus species make mycorrhizal associations with different plant species in the families Casuarinaceae, Dipterocarpaceae, Fagaceae, Myrtaceae and Pinaceae (Marx 1977;Malloch & Kuja 1979).…”

Section: Introductionmentioning

confidence: 99%

Pisolithus albus (Sclerodermataceae), a first record from Egypt

Abdel-Aziz,

Bakhit

2023

Plant and Fungal Syst.

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Pisolithus albus (Basidiomycota, Sclerodermataceae) is reported here for the first time from Egypt where it was found on the Karaman Island in Sohag Governorate. It was found forming an ectomycorrhizal association with Eucalyptus occidentalis and Vachellia nilotica. The fungus was also reported near two potentially new hosts: Paspalum distichum and Dodonaea viscosa; however, the mycorrhizal connections with these two hosts is not proven. Phylogenetic analyses for the combined ITS and LSU rDNA sequences dataset placed the Egyptian specimen within the Pisolithus albus complex clade. It is described, illustrated, and phylogenetically analyzed in this article. Various hosts and locations of the taxon are reviewed.

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“…The genus is characterized by fruit bodies of variable shape and size, often with a well-developed rooting base (Jaouni et al, 2015). Most species are widely distributed across temperate to tropical regions and form ectomycorrhizal (ECM) associations with a variety of woody plants (Marx, 1977;Chambers, Cairney, 1999;Rusevska et al, 2015). The species of Pisolithus occur in a range of habitats including forests, orchards, urban sites, and eroded soils (Marx, 1977;Malloch, Kuja, 1979;Castellano, Trapp, 1991;Salah et al, 2009;Phosri et al, 2012;Martín et al, 2013;Jaouni et al, 2015;Rusevska et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The first record of Pisolithus arhizus (Sclerodermataceae, Basidiomycota) in Central Asia

Islomiddinov,

Mustafaev,

Shirqulova

et al. 2023

Ukr. Bot. J.

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Pisolithus is a genus of gasteroid mycorrhizal symbionts associated with trees of several families of angiosperms and gymnosperms and distributed almost worldwide. Here we report a new record of Pisolithus arhizus from Tashkent, Uzbekistan, the first record of this species in Central Asia. The fruit bodies of P. arhizus were collected in several locations within the city and identified based on morphological characters. The ectomycorrhizal fungus formed symbiotic relationships with Juniperus sp. and Quercus sp. We provide its morphological description and photographs and also discuss our findings in the context of previously known records of this species.

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DNA barcoding is an effective tool for differentiating <I>Pisolithus</I> species from Macedonia

Cited by 6 publications

References 0 publications

Fungal DNA barcode (ITS nrDNA) reveals more diversity than expected in Tulostoma from Macedonia

Fungal DNA barcode (ITS nrDNA) reveals more diversity than expected in Tulostoma from Macedonia

Pisolithus albus (Sclerodermataceae), a first record from Egypt

The first record of Pisolithus arhizus (Sclerodermataceae, Basidiomycota) in Central Asia

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