DNA barcoding for seahorse identification and its potential as antioxidant and stimulant indicator

Nurilmala, Mala; Sarı, Elmas; Abdullah, Asadatun; Hizbullah, Hanifah Husein; Butet, Nurlisa A.; Sudrajat, Agus Oman

doi:10.1088/1755-1315/404/1/012002

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…The longer branches indicated the species has a greater DNA base difference than other species. Conversely, the shorter a branch shows the difference in nucleotide bases and the less evolutionary rate (Nurilmala et al, 2020).…”

Section: Molecular Phylogeneticsmentioning

confidence: 99%

Molecular characterization and antioxidant activity of Volkameria inermis L.

Sundari

Shanthi

2022

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The present investigation aimed to study the genomic characterization and free radical scavenging potential of a traditional plant Volkameria inermis. A chloroplast genome rbcL was used in molecular characterization and it revealed the phylogeny and evolutionary affinities of V. inermis with the outgroups of the family through BLAST search based on Neighbor-Joining (NJ) analysis. The free radical scavenging potential of various extracts of V. inermis was observed at different concentrations (20, 40, 60, 80 and 100 μg/mL) in DPPH and ABTS in vitro models. Ascorbic acid (DPPH) and Trolox (ABTS) were used as standard. In DPPH assay, among the different extracts (hexane, chloroform, acetone, ethanol, methanol and water) tested, methanol showed maximum scavenging activity with the IC50 value of 28.33μg/mL followed by acetone with the IC50 value of 31.937μg/mL. The other extracts, ethanol and water extracts showed moderate activity with the IC 50 value of 82.558 μg/mL and 81.758μg/mL; chloroform and hexane showed very lower antioxidant activity and the IC50 values are 518.776μg/mL, 1066.16μg/mL accordingly. In ABTS assay, methanol was observed as a highly active extracts with the IC50 value of 70.196μg/mL followed by ethanol extract (103.078μg/mL). The results showed that the methanol extract exhibits strong antioxidant activity in DPPH and ABTS assays. The scavenging efficiency showed dose dependent increase in concentration absorption compared to Ascorbic acid and Trolox. Our findings provide the molecular identification and evidence for the potential of Volkameria inermis as a source of natural antioxidants.

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Section: Molecular Phylogeneticsmentioning

confidence: 99%

Molecular characterization and antioxidant activity of Volkameria inermis L.

Sundari

Shanthi

2022

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“…Mitochondrial DNA (mtDNA) is often used in DNA barcoding of many organisms, including fish (Abdullah et al 2019;Nurilmala et al 2020) because of the relatively small genome size. Also, mtDNA undergoes faster evolution making it more effective for the identification of adjacent interspecies (Amaral et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

Nurilmala¹,

Ajitama²,

Jacoeb³

et al. 2020

Biodiversitas

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Abstract. Nurilmala M, Atjitama Y, Jacob AM, Indarwati AR, Nugraha R. 2020. Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers. Biodiversitas 21: 5650-5656. Shrimp crackers are snacks made from starch with the addition of shrimp and other ingredients. Authentication of shrimp crackers becomes a quality control method in line with its traceability since the morphological characters are not recognized in those products. The failure of the authentication will lead to adulteration of shrimp products. The study aimed to design shrimp-specific polymerase chain reaction (PCR) primers by and used them to authenticate shrimp cracker products to assure their traceability. The primers were designed from cytochrome oxidase subunit I (COI) gene with a GC content of 50% and a melting temperature of 57 °C. The amplicon had a length of 613 bp. Fourteen shrimp crackers having DNA concentrations 0.3 to 8.1 ng/µL were evaluated. Eight out of fourteen DNA samples were successfully amplified using the specific primer and could be visualized on the agarose gel at 500-750 bp. Those DNA samples were identified belonging to shrimp species, namely Litopenaeus vannamei, Metapenaeus ensis, and Fenneropenaeus merguiensis with 95% to 100% identity. Meanwhile, the other six DNA samples underwent PCR using universal primers. One sample was amplified and identified as fish (Sphyraena flavicauda). These results indicate illegal substitution of shrimp using unidentified species occurred in shrimp crackers.

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Diversity of Seahorse Species (Hippocampus spp.) in the International Aquarium Trade

Koning

Hoeksema

2021

Diversity

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Seahorses (Hippocampus spp.) are threatened as a result of habitat degradation and overfishing. They have commercial value as traditional medicine, curio objects, and pets in the aquarium industry. There are 48 valid species, 27 of which are represented in the international aquarium trade. Most species in the aquarium industry are relatively large and were described early in the history of seahorse taxonomy. In 2002, seahorses became the first marine fishes for which the international trade became regulated by CITES (Convention for the International Trade in Endangered Species of Wild Fauna and Flora), with implementation in 2004. Since then, aquaculture has been developed to improve the sustainability of the seahorse trade. This review provides analyses of the roles of wild-caught and cultured individuals in the international aquarium trade of various Hippocampus species for the period 1997–2018. For all species, trade numbers declined after 2011. The proportion of cultured seahorses in the aquarium trade increased rapidly after their listing in CITES, although the industry is still struggling to produce large numbers of young in a cost-effective way, and its economic viability is technically challenging in terms of diet and disease. Whether seahorse aquaculture can benefit wild populations will largely depend on its capacity to provide an alternative livelihood for subsistence fishers in the source countries. For most species, CITES trade records of live animals in the aquarium industry started a few years earlier than those of dead bodies in the traditional medicine trade, despite the latter being 15 times higher in number. The use of DNA analysis in the species identification of seahorses has predominantly been applied to animals in the traditional medicine market, but not to the aquarium trade. Genetic tools have already been used in the description of new species and will also help to discover new species and in various other kinds of applications.

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DNA barcoding for seahorse identification and its potential as antioxidant and stimulant indicator

Cited by 3 publications

References 6 publications

Molecular characterization and antioxidant activity of Volkameria inermis L.

Molecular characterization and antioxidant activity of Volkameria inermis L.

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

Diversity of Seahorse Species (Hippocampus spp.) in the International Aquarium Trade

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