DNA barcoding for identification of mosquitoes (Diptera: Culicidae) from the Ryukyu Archipelago, Japan

Abstract

Abstract:e utility of cytochrome c oxidase subunit I (COI) DNA barcoding for the identi cation of 37 mosquito species belonging to the genera Anopheles (6 spp.), Aedes (14 spp.) and Culex (17 spp.) including subspecies from nine islands of the Ryukyu Archipelago, Japan was examined. A 658 bp fragment of the COI gene was sequenced from 228 specimens. Nucleotide sequence divergences were calculated using the Kimura-two parameter (K2P) distance model and a neighbor-joining (NJ) phylogenetic tree analysis was performed to provide a graphic display of the patterns in divergence among species. e mean intraspeci c variations of 35 species including the medically important vector species showed a less than 2 range with 0 1.5 divergences, but higher divergences of more than 2 were detected in Ae. aureostriatus okinawanus Bohart (2.2 ) and Cx. hayashii ryukyuanus Tanaka et al. (3.3 ). ey showed di erences in divergence between specimens from di erent islands. All 37 species were discriminated as distinct clusters in the NJ tree. e results in this study prove the utility of DNA barcoding for identi cation of mosquitoes from the Ryukyu Archipelago, Japan.

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

“…DNA barcoding studies conducted in Pakistan and China reported 0–2.4 and 0–1.67% divergence ranges, respectively, similar to observations of the current study [5, 7, 54]. An intra-specific divergence of 1.84% has reported among the haplotypes of An.…”

Mosquito Biodiversity Patterns Around Urban Environments in South-Central Okinawa Island, Japan

“…Ecological studies on the Anopheles Hyrcanus group of Okinawa (Rueda et al 2005, Taira et al 2012 suggest that the species we found was An. sinsensis Weidemann.…”

“…These 2 species can be morphologically separated based on the presence of a fringe pale spot in the wings and the presence of abundant scales on the midcoxa (Tanaka et al 1979, Toma andMiyagi 1986), which according to DNA barcoding are highly precise and accurate identification characters unique to An. sinensis (Taira et al 2012). Thus, we are certain our samples do not belong to An.…”

“…lesteri, given the presence of both the fringe pale spot in the wings and abundant scales on the midcoxa of our samples. However, additional molecular tests (Li et al 2005) are required to see if our samples (Rueda et al 2005, Taira et al 2012). We collected 6 species that are vectors of medically important pathogens: Ae.…”

Identification of Three Distinct Groups of <i>Anopheles lindesayi</i> in Japan by Morphological and Genetic Analyses

“…Notably, the largest K2P distance (9.13%) observed between specimens from Hokkaido (E-Japan) and Nagasaki (W-Japan) is much greater than the intra-specific K2P divergence (<2%) based on COI barcoding region of mosquitoes (35,36). Maekawa et al (34) also reported a high K2P distance (8.5%) between specimens from Hokkaido (E-Japan) and Wakayama (W-Japan).…”