DNA Barcoding for Honey Biodiversity

Valentini, Alice; Miquel, Christian; Taberlet, Pierre

doi:10.3390/d2040610

Cited by 92 publications

(77 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While plant identification from DNA has presented numerous challenges (CBOL 2009), it has the advantage of providing finer definition of species‐specific foraging preferences for plant families whose pollen is difficult to distinguish morphologically (Bell et al., 2016; Bruni et al., 2015; Hawkins et al., 2015; Kraaijeveld et al., 2015; Pornon, Andalo, Burrus, & Escaravage, 2017). DNA sequencing has been used successfully to identify the foraging preference of honeybees and the floral composition of their honey (Bruni et al., 2015; De Vere et al., 2017; Galimberti et al., 2014; Hawkins et al., 2015; Jain, Jesus, Marchioro, & Araújo, 2013; Valentini, Miquel, & Taberlet, 2010). Several studies have also used pollen DNA sequencing to identify solitary bee foraging preferences (Sickel et al., 2015; Wilson, Sidhu, Levan, & Holway, 2010).…”

Section: Introductionmentioning

confidence: 99%

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Gresty

Clare

Devey

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Floral foraging resources are valuable for pollinator conservation on farmland, and their provision is encouraged by agri‐environment schemes in many countries. Across Europe, wildflower seed mixtures are widely sown on farmland to encourage pollinators, but the extent to which key pollinator groups such as solitary bees exploit and benefit from these resources is unclear. We used high‐throughput sequencing of 164 pollen samples extracted from the brood cells of six common cavity‐nesting solitary bee species (Osmia bicornis, Osmia caerulescens, Megachile versicolor, Megachile ligniseca, Megachile centuncularis and Hylaeus confusus) which are widely distributed across the UK and Europe. We documented their pollen use across 19 farms in southern England, UK, revealing their forage plants and examining the structure of their pollen transport networks. Of the 32 plant species included currently in sown wildflower mixes, 15 were recorded as present within close foraging range of the bees on the study farms, but only Ranunculus acris L. was identified within the pollen samples. Rosa canina L. was the most commonly found of the 23 plant species identified in the pollen samples, suggesting that, in addition to providing a nesting resource for Megachile leafcutter bees, it may be an important forage plant for these species. Higher levels of connectance and nestedness were characteristic of pollen transport networks on farms with abundant floral resources, which may increase resilience to species loss. Our data suggest that plant species promoted currently by agri‐environment schemes are not optimal for solitary bee foraging. If a diverse community of pollinators is to be supported on UK and European farmland, additional species such as R. canina should be encouraged to meet the foraging requirements of solitary bees.

show abstract

Section: Introductionmentioning

confidence: 99%

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Gresty

Clare

Devey

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Hence, removal of sugar from the honey samples will be important during the DNA extraction process. On the other hand, the high carbohydrate concentration in the honey helps DNA preservation, 11 as sugars stabilize nucleic acids, 12 and honey provides an airtight seal that prevents oxygen from entering and thus, preserving DNA from being degraded. 11 The presence of polyphenolic content makes the isolation of high-quality nucleic acids problematic; in addition, residual polyphenolics interfere in enzymatic reactions, such as PCR and endonuclease restriction digestion.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the high carbohydrate concentration in the honey helps DNA preservation, 11 as sugars stabilize nucleic acids, 12 and honey provides an airtight seal that prevents oxygen from entering and thus, preserving DNA from being degraded. 11 The presence of polyphenolic content makes the isolation of high-quality nucleic acids problematic; in addition, residual polyphenolics interfere in enzymatic reactions, such as PCR and endonuclease restriction digestion. 13 In the present study, initial incubation of the sample dissolved in water resulted in removal of sugar and polyphenols as supernatants after the centrifugation process.…”

Section: Discussionmentioning

confidence: 99%

Protocol for Optimal Quality and Quantity Pollen DNA Isolation from Honey Samples

Lalhmangaihi¹,

Ghatak²,

Laha³

et al. 2014

J Biomol Tech

View full text Add to dashboard Cite

The present study illustrates an optimized sample preparation method for an efficient DNA isolation from low quantities of honey samples. A conventional PCR-based method was validated, which potentially enables characterization of plant species from as low as 3 ml bee-honey samples. In the present study, an anionic detergent was used to lyse the hard outer pollen shell, and DTT was used for isolation of thiolated DNA, as it might facilitate protein digestion and assists in releasing the DNA into solution, as well as reduce cross-links between DNA and other biomolecules. Optimization of both the quantity of honey sample and time duration for DNA isolation was done during development of this method. With the use of this method, chloroplast DNA was successfully PCR amplified and sequenced from honey DNA samples.

show abstract

“…Several chemical methods were also proposed, such as aroma compounds, free amino acids or minerals and trace elements. Even if all these methods work well for identifying the geographic origins and for distinguishing honey with different botanical origins, they provide only limited information on the plant composition [51]. In fact, it is not always possibile to recognize individual species because some pollen is not well distinguishable by its morphological traits (e.g., Campulanaceae and Lamiaceae) [50].…”

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 99%

“…Recently, DNA barcoding was proposed as molecular tool for honey traceability exploitable in distinct steps of the supply chain. Some reserachers demonstrated that standard barcodes, such as trnL gene intron and rbcL gene region, are suitable for identifying plant traces from different honey samples, but anable for determining the botanical composition of individual honey species [50,51]. A synergism between multiple barcode markers among those conventionally used is in fact necessary to differentiate congeneric taxa.…”

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

View full text Add to dashboard Cite

DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

show abstract

DNA Barcoding for Honey Biodiversity

Cited by 92 publications

References 29 publications

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Flower preferences and pollen transport networks for cavity‐nesting solitary bees: Implications for the design of agri‐environment schemes

Protocol for Optimal Quality and Quantity Pollen DNA Isolation from Honey Samples

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

Contact Info

Product

Resources

About