DNA Barcoding for Discriminating the Economically Important<i>Cinnamomum verum</i>from Its Adulterants

Swetha, V. P.; Parvathy, V. A.; Sheeja, T. E.; Sasikumar, B.

doi:10.1080/08905436.2014.931239

Cited by 44 publications

(22 citation statements)

References 32 publications

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“…DNA barcoding has been used for identifying medicinal plants [ 64 ] and industrial quality assurance [ 65 ], such as for Smithia conferta Sm. [ 66 ], turmeric [ 67 ], Crocus sativus [ 68 , 69 ], Peucedanum praeruptorum [ 70 ], radix astragali [ 71 , 72 ], Cinnamomum verum [ 73 ], Sabia parviflora [ 74 ], Valeriana jatamansi [ 75 ], sandalwood [ 76 ], and Hippophae [ 30 ], which supports our findings. To date, it has been difficult to completely authenticate PR and its related products without relying on morphological characterization.…”

Section: Discussionsupporting

confidence: 82%

DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China

et al. 2018

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Polygonati rhizoma (PR), a traditional medicinal and edible product with various bioactive components (Polygonatum polysaccharides, saponins, phenols, and flavonoids), is widely consumed in China. However, other species with morphological characteristics similar to those of the actual components are being used to replace or adulterate PR, causing issues with quality control and product safety. The morphological similarity of PR and its substitutes makes classic morphological identification challenging. To address this issue, DNA barcoding-based identification using ITS2 and psbA-trnH sequences was applied in this study to evaluate the efficiency and accuracy of this approach in identifying PR samples collected from 39 different regions in China. The identification of PR by this method was confirmed by other methods (phylogeny-based and character-based methods), and all the samples were clearly and accurately distinguished. This study highlights the efficient and accurate nature of DNA barcoding in PR identification. Applying this technique will provide a means to differentiate PR from other altered formulations, thus improving product quality and safety for consumers of PR and its products.

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Section: Discussionsupporting

confidence: 82%

DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China

et al. 2018

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“…Similarly, genetic identifications using DNA barcodes for wild-collected medicinal plants [67], market adulterants in certified natural and commercial products [68], and genetically modified crops [69] are becoming more common. These applied uses of DNA barcodes for conservation and commercial purposes will undoubtedly increase in the future, especially as sequencing technology becomes simpler and less expensive.…”

Section: Reviewmentioning

confidence: 99%

DNA barcodes for ecology, evolution, and conservation

Kress

García‐Robledo

Uriarte

et al. 2015

Trends in Ecology & Evolution

425

336

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“…However, as previously mentioned, Cinnamomum is primarily found in tropical and subtropical Asia and Australia, and therefore the collection of specimens for examination has been limited. Several techniques used to identify Cinnamomum plants have been reported, including those based on RAPD (Joy and Maridass 2008; Sudmoon et al 2014), chloroplast DNA (e.g., trnL - F, trnL intron, matK, rbcL and trnH - psbA ) sequences (Sudmoon et al 2014; Kojoma et al 2002; Abeysinghe et al 2009; Swetha et al 2014a, b), and ITS nucleotide sequences (Ho et al 2015; Abeysinghe et al 2009). However, most of the research has focused on specimens from a limited number of species.…”

Section: Discussionmentioning

confidence: 99%

“…In Sri Lanka and India, C. verum is cultivated as one of the most important spices, and is treated as true cinnamon that is widely used in the food and cosmetic industries (Swetha et al 2014a, b). C. cassia, referred to as cassia cinnamon or Chinese cinnamon in Sri Lanka, is treated as an adulterant of C. verum (Thomas and Duethi 2001).…”

Section: Introductionmentioning

confidence: 99%

Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker

Doh

Lee

2016

Genes Genom

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In this study, we identified and evaluated the genetic relationships among Cinnamomum plants, which are used in traditional medicine. We also attempted to monitor the distribution of traditional medicines derived from Cinnamomum cassia by using DNA barcoding and a species-specific DNA marker. Plants of the genus Cinnamomum, and in particular C. cassia, are commonly used as medicinal herbs in the form of Cinnamomi Ramulus, Cinnamomi Cortex, and Cassiae Cortex Interior. However, it is difficult to distinguish among different Cinnamomum species based on morphological features, and so to overcome this limitation, nucleotide sequences of the internal transcribed spacer (ITS) region of Cinnamomum DNA were determined and compared. On the basis of the discrepancy in determined ITS sequences, a 408-bp product, amplified by the primer pair CC F1/CC R3, was developed as a C. cassia-specific DNA marker. Using the developed DNA marker in combination with the ITS 2 nucleotide sequence, we monitored imported and commercially supplied medicinal products derived from Cinnamomum plants in markets in Korean, China, and Japan. The results revealed that most of the specimens monitored were derived from C. cassia.Electronic supplementary materialThe online version of this article (doi:10.1007/s13258-016-0476-5) contains supplementary material, which is available to authorized users.

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DNA Barcoding for Discriminating the Economically ImportantCinnamomum verumfrom Its Adulterants

Cited by 44 publications

References 32 publications

DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China

DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China

DNA barcodes for ecology, evolution, and conservation

Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker

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