DNA barcoding common non-native freshwater fish species in Turkey: Low genetic diversity but high population structuring

Abstract

Negative impacts of introduced non-native freshwater species on native species have been increasingly recognized in the world as well as in Turkey. However, there has been relatively little attention on genetic characterization of alien freshwater fishes in their non-native distribution range and virtually no study has been conducted in Turkey despite its crucial importance in invasion biology. The purpose of this study was to elucidate genetic diversity of common non-native freshwater fish species (Carassius auratus, Carassius gibelio, Gambusia holbrooki, Lepomis gibbosus, and Pseudorasbora parva) using mitochondrial Cytochrome c oxidase subunit I (COI) sequences; known as DNA barcodes. Through the whole dataset, seventeen haplotypes (haplotype diversity = 0.8908) were found containing 145 COI sequences. Mean Kimura two-parameter genetic distances were calculated as 0.209 for interspecific distance and 0.009 for intraspecific variation. COI barcode diversity among populations of the same species was found to be low, especially for C. gibelio, G. holbrooki, and L. gibbosus populations which were 0.5%, 0.6%, and 0.3%, respectively. Our results clearly demonstrate the effectiveness of the DNA barcoding approach both for identifications at species level and revealing intraspecific variation among populations, which could be used for effective management measures for invasive species and conservation strategies for indigenous and endemic species.

DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River

“…; Keskin et al. ; Loh et al. ) and is regularly used for a variety of applications, such as fishery management, biodiversity assessments and conservation (Triantafyllidis et al.…”

“…Sequences for the same species are generally considered to be correctly identified when they form a monophyletic cluster on a neighbor-joining (NJ) tree with intraspecific distances that are below a given threshold (Srivathsan and Meier 2012). At present, this approach has proven to be highly efficient and reliable in many fish groups (Ward et al 2005;Hubert et al 2008Hubert et al , 2010Rock et al 2008;Keskin et al 2013;Loh et al 2014) and is regularly used for a variety of applications, such as fishery management, biodiversity assessments and conservation (Triantafyllidis et al 2011;Weigt et al 2012;Keskin et al 2013;Loh et al 2014;Shen et al 2016).…”

DNA barcoding the ichthyofauna of the Yangtze River: Insights from the molecular inventory of a mega‐diverse temperate fauna

“…Recent large-scale assessments of diverse ichthyofauna confirmed that the use of the cytochrome oxidase I gene successfully capture a large portion of the alpha diversity for well-known fauna (April, Mayden, Hanner, & Bernatchez, 2011;Hubert, Hanner, & Holm, 2008a;Ward, Zemlak, Innes, Last, & Hebert, 2005a) but also helped in highlighting unrecognized (i.e., cryptic) diversity (Durand, Hubert, Shen, & Borsa, 2017;Hubert, Meyer, & Bruggemann, 2012;Jaafar, Taylor, Mohd Nor, Bruyn, & Carvalho, 2012;Pereira, Pazian, Hanner, Foresti, & Oliveira, 2011;Rock, Costa, & Walker, 2008;Winterbottom, Hanner, Burridge, & Zur, 2014). Landscape features such as fragmentation, however, proved to be a challenge to accurately delimit species through DNA barcodes (Geiger, Herder, & Monaghan, 2014;Keskin, Agdamar, & Tarkan, 2013;Knebelsberger, Dunz, Neumann, & Geiger, 2015). In the context of the YR ichthyofauna, a preliminary assessment of the effectiveness of DNA barcoding in identifying YR freshwater fishes confirmed its potential for conservation purposes (Shen, Guan, Wang, & Gan, 2016b;Shen, Kang, Chen, & He, 2016a).…”

Demersal Fish Diversity and Molecular Taxonomy in the Bering Sea and Chukchi Sea

“…Therefore, the use of DNA barcodes as an accurate and effective method of species identi cation is currently favored by an increasing number of researchers. Recent studies have indicated that this technology is highly reliable and e cient in many sh groups, including freshwater shes (Keskin et al 2013) Moreover, it is widely used in a variety of elds, such as biodiversity assessment, sh larva identi cation and shery management (Gao 2015;Panprommin et al 2020).…”