DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand

Sumruayphol, Suchada; Apiwathnasorn, Chamnarn; Ruangsittichai, Jiraporn; Sriwichai, Patchara; Attrapadung, Siriluck; Samung, Yudthana; Dujardin, Jean‐Pierre

doi:10.1016/j.actatropica.2016.03.010

Cited by 43 publications

(81 citation statements)

References 36 publications

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“…Indeed, when calculated on the standard COI sequence (Hebert et al, 2003) employed for DNA barcoding, the maximum intraspecific divergence was 0.012 (eight mutations in 658 bp), a value in line with those recently reported for A. scutellaris (0.008) and A. aegypti (0.022), but much greater than the value previously reported for A. albopictus (0.002; Sumruayphol et al, 2016). …”

Section: Resultssupporting

confidence: 86%

The Worldwide Spread of the Tiger Mosquito as Revealed by Mitogenome Haplogroup Diversity

et al. 2016

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In the last 40 years, the Asian tiger mosquito Aedes albopictus, indigenous to East Asia, has colonized every continent except Antarctica. Its spread is a major public health concern, given that this species is a competent vector for numerous arboviruses, including those causing dengue, chikungunya, West Nile, and the recently emerged Zika fever. To acquire more information on the ancestral source(s) of adventive populations and the overall diffusion process from its native range, we analyzed the mitogenome variation of 27 individuals from representative populations of Asia, the Americas, and Europe. Phylogenetic analyses revealed five haplogroups in Asia, but population surveys appear to indicate that only three of these (A1a1, A1a2, and A1b) were involved in the recent worldwide spread. We also found out that a derived lineage (A1a1a1) within A1a1, which is now common in Italy, most likely arose in North America from an ancestral Japanese source. These different genetic sources now coexist in many of the recently colonized areas, thus probably creating novel genomic combinations which might be one of the causes of the apparently growing ability of A. albopictus to expand its geographical range.

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Section: Resultssupporting

confidence: 86%

The Worldwide Spread of the Tiger Mosquito as Revealed by Mitogenome Haplogroup Diversity

et al. 2016

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“…Geometric morphometrics is increasingly applied to medically and economically important insects to distinguish morphologically similar species, especially cryptic taxa, and to detect intraspecific variation (Dujardin 2008, Ruangsittichai et al 2011, Lorenz et al 2012, Dujardin and Kitthawee 2013, Morales Vargas et al 2013, Demari-Silva et al 2014, Jaramillo-O et al 2015, Sumruayphol et al 2016). Wing veins provide many well-defined landmarks suitable for the landmark-based approach (Villegas et al 2002).…”

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confidence: 99%

Landmark and outline-based geometric morphometrics analysis of three Stomoxys flies (Diptera: Muscidae)

Changbunjong¹,

Sumruayphol²,

Weluwanarak³

et al. 2016

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Adult flies of the genus Stomoxys Geoffroy, 1762 (Diptera: Muscidae), especially S. pullus Austen, 1909, S. uruma Shinonaga et Kano, 1966 and S. indicus Picard, 1908, are morphologically similar and sometimes difficult to distinguish when using external morphological characteristics. These species may act as vectors and/or potential vectors of many pathogens (virus, bacteria and protozoa). Their correct identification is important to target the vectors involved in the transmission of the pathogens and also helps in the fly control program.The aim of the present study was to distinguish three species which are difficult to separate using traditional diagnostic characters for species of Stomoxys such as colour patterns and body proportions. Modern morphometrics, both landmark and outline-based, was used to access wing geometry of S. pullus, S. uruma and S. indicus. A total of 198 and 190 wing pictures were analysed for landmark-and outline-based approaches, respectively. Wing shape was able to separate species and sexes of the three Stomoxys flies with highly significant difference of Mahalanobis distances. The cross-validated classification scores ranged from 76% to 100% for landmark and 77% to 96% for outline-based morphometrics. The geometry of wing features appears to be a very useful, low-cost tool to distinguish among the vectors S. pullus, S. uruma and S. indicus.

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“…In Diptera, the wing measurements were used successfully for the quantification of both within and between species variations (Brown 1980; Klingenberg et al 1998; Alves and Bélo 2002; Hall et al 2014; Siomava et al 2016). The wing venation differs markedly between Diptera species, and it can be used for identification of mosquitoes (Dujardin 2011; Sumruayphol et al 2016), tephritid flies (Van Cann et al 2015), tsetse flies (Kaba et al 2016), screwworm flies (Lyra et al 2010), and stable flies (Changbunjong et al 2016). Our study is the first extensive attempt to investigate the usefulness of wing measurements for the identification of dipterans reported from animal carrion and dead human bodies.…”

Section: Discussionmentioning

confidence: 99%

Identification of Muscidae (Diptera) of medico-legal importance by means of wing measurements

2017

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Cadavers attract numerous species and genera of Muscidae, both regular elements of carrion insect assemblages, and accidental visitors. Identification of adult Muscidae may be considered difficult, particularly by non-experts. Since species identification is a vital first step in the analysis of entomological material in any forensic entomology orientated experiment and real cases, various alternative methods of species identification have been proposed. We investigated possibility of semiautomated identification by means of wing measurements as an alternative for classic morphology and DNA-based approaches. We examined genus-level identification success for 790 specimens representing 13 genera of the most common European cadavers visiting Muscidae. We found 99.8% of examined specimens correctly identified to the genus-level. Without error, the following were identified: Azelia, Eudasyphora, Graphomya, Hydrotaea, Musca, Muscina, Mydaea, Neomyia, Polietes, Stomoxys and Thricops. Genus-level misidentifications were found only in Helina and Phaonia. Discrimination of examined material on the species level within Hydrotaea (318 specimens representing eight species) and Muscina (163 specimens representing four species) showed lower, yet still high average identification success, 97.2 and 98.8%, respectively. Our results revealed relatively high success in both genus and species identification of Muscidae of medico-legal importance. Semiautomated identification by means of wing measurements can be used by non-experts and does not require sophisticated equipment. This method will facilitate the identification of forensically relevant muscids in comparison to more difficult and more time-consuming identification approaches based on taxonomic keys or DNA-based methods. However, for unambiguous identification of some taxa, we recommend complementary use of identification keys.

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DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand

Cited by 43 publications

References 36 publications

The Worldwide Spread of the Tiger Mosquito as Revealed by Mitogenome Haplogroup Diversity

The Worldwide Spread of the Tiger Mosquito as Revealed by Mitogenome Haplogroup Diversity

Landmark and outline-based geometric morphometrics analysis of three Stomoxys flies (Diptera: Muscidae)

Identification of Muscidae (Diptera) of medico-legal importance by means of wing measurements

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