DNA Barcoding and Morphological Analyses Reveal a Cryptic Species of Miniopterus from India and Sri Lanka

Kusuminda, Tharaka; Mannakkara, Amani; Ukuwela, Kanishka D. B.; Крускоп, С.В.; AMARASINGHE, CHAMARA; Saikia, Uttam; Venugopal, P. Dilip; KARUNARATHNA, MATHISHA; Gamage, Rajika; Rüedi, Manuel; Csorba, Gábor; Yapa, Wipula; Patterson, Bruce D.

doi:10.3161/15081109acc2022.24.1.001

Cited by 7 publications

(4 citation statements)

References 60 publications

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“…In summary, the virome composition of different sample types obtained from M. fuliginosus bats in Sri Lanka was analyzed for the first time. Recent DNA barcoding and morphological studies on this species suggest that the Miniopterus bats inhabiting the island of Sri Lanka are in fact a new species of bat, not described hitherto and named as Miniopterus phillipsi [65].…”

Section: Discussionmentioning

confidence: 89%

Comparative virome analysis of individual shedding routes of Miniopterus fuliginosus bats inhabiting the Wavul Galge Cave, Sri Lanka

Muzeniek

Perera

Siriwardana

et al. 2022

Preprint

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Bats are described as the natural reservoir host for a wide range of viruses. Although an increasing number of bat-associated, potentially human pathogenic viruses were discovered in the past, the full picture of the bat viromes is not explored yet. In this study, the virome composition from Miniopterus fuliginosus bats inhabiting the Wavul Galge cave, Sri Lanka, was analyzed. To assess different possible shedding routes, oral swabs, feces and urine were collected and analyzed individually by using metagenomic NGS. The data obtained was further evaluated by using phylogenetic reconstructions. Two different alphacoronavirus strains were detected in feces and urine samples. Furthermore, a paramyxovirus was detected in urine samples. Sequences related to Picornaviridae, Retroviridae, Iflaviridae, unclassified Riboviria and Astroviridae were identified in feces samples, and further sequences related to Astroviridae in urine samples. No further viruses were detected in oral swab samples. The comparative virome analysis in this study revealed a diversity in the virome composition between the collected sample types which also represent different potential shedding routes for the detected viruses. At the same time, several viruses were detected for the first time in bats in Sri Lanka. The detection of two different coronaviruses in the samples indicates the potential general persistence of this virus species in M. fuliginosus bats. Based on phylogenetics, the identified viruses are closer related to bat-associated viruses with comparably low human pathogenic potential. In further studies, the seasonal variation of the virome will be analyzed to identify possible shedding patterns for particular viruses.

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Section: Discussionmentioning

confidence: 89%

Comparative virome analysis of individual shedding routes of Miniopterus fuliginosus bats inhabiting the Wavul Galge Cave, Sri Lanka

Muzeniek

Perera

Siriwardana

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In summary, the virome composition of different sample types obtained from M. phillipsi bats in Sri Lanka was analyzed for the first time. Recently, DNA barcoding and morphological studies on this species suggest that the Miniopterus bats inhabiting the island of Sri Lanka are in fact a new species of bat not described hitherto and named Miniopterus phillipsi 66 . All studies on Miniopterus bats from Sri Lanka, conducted before the renaming of the species, identified them as Miniopterus fuliginosus due to lack of more precise information in the database.…”

Section: Discussionmentioning

confidence: 97%

Comparative virome analysis of individual shedding routes of Miniopterus phillipsi bats inhabiting the Wavul Galge cave, Sri Lanka

Muzeniek

Perera

Siriwardana

et al. 2023

Sci Rep

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Bats are described as the natural reservoir host for a wide range of viruses. Although an increasing number of bat-associated, potentially human pathogenic viruses were discovered in the past, the full picture of the bat viromes is not explored yet. In this study, the virome composition of Miniopterus phillipsi bats (formerly known as Miniopterus fuliginosus bats in Sri Lanka) inhabiting the Wavul Galge cave, Sri Lanka, was analyzed. To assess different possible excretion routes, oral swabs, feces and urine were collected and analyzed individually by using metagenomic NGS. The data obtained was further evaluated by using phylogenetic reconstructions, whereby a special focus was set on RNA viruses that are typically associated with bats. Two different alphacoronavirus strains were detected in feces and urine samples. Furthermore, a paramyxovirus was detected in urine samples. Sequences related to Picornaviridae, Iflaviridae, unclassified Riboviria and Astroviridae were identified in feces samples and further sequences related to Astroviridae in urine samples. No viruses were detected in oral swab samples. The comparative virome analysis in this study revealed a diversity in the virome composition between the collected sample types which also represent different potential shedding routes for the detected viruses. At the same time, several novel viruses represent first reports of these pathogens from bats in Sri Lanka. The detection of two different coronaviruses in the samples indicates the potential general persistence of this virus species in M. phillipsi bats. Based on phylogenetics, the identified viruses are closely related to bat-associated viruses with comparably low estimation of human pathogenic potential. In further studies, the seasonal variation of the virome will be analyzed to identify possible shedding patterns for particular viruses.

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“…Miniopterus eschscholtzii was formerly acknowledged as a subspecies of M. schreibersii. However, subsequent molecular studies resulted in its reclassification as a distinct species (Akmali et al 2015;MMD 2021;Kusuminda et al 2022;Simmons & Cirranello 2023). FmaxE values of the Philippine endemic Miniopterus eschscholtzii recorded in this study were within the range of obtained values recorded from other localities within the Philippines: 48.5 kHz in Bohol Island (Phelps et al 2018), 45.6 kHz in Mt.…”

Section: Fm Bats (Miniopteridae and Vespertilionidae)mentioning

confidence: 99%

Echolocation call characterization of insectivorous bats from caves and karst areas in southern Luzon Island, Philippines

Duco,

de Guia,

Dimalibot

et al. 2023

J. Threat. Taxa

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Bats are excellent bioindicators and are increasingly used to assess ecosystem health and monitor changes in the environment. Due to increased awareness of the potential transmission of pathogens from bats to humans and recognizing the limitations of traditional bat sampling methods, the use of of non-invasive sampling techniques such as bat recorders were recommended for field-based monitoring studies. In the Philippines, however, bat bioacoustics is still a growing field, and the scarcity of acoustic data hinders the use of echolocation calls to conduct accurate inventories and population monitoring of echolocating bats. Here, we recorded and characterized echolocation calls of insectivorous bats from caves and karst areas located in southern Luzon Island, Philippines. In addition, we compared our results with other studies performed within and outside the country to identify possible regional and local variation in acoustic characters for some species. A total of 441 echolocation calls were recorded from six bat families: Hipposideridae (five species), Rhinolophidae (five species), Vespertilionidae (three species), Miniopteridae (two species), Megadermatidae (one species), and Emballonuridae (one species). Discriminant function analyses (DFA) with leave-one-out cross validation correctly classified bats emitting calls dominated with a constant frequency (CF) component (rhinolophids and hipposiderids) with >97% success and those producing frequency modulated (FM) calls (Miniopteridae and Vespertilionidae) with 88.9% success. We report echolocation calls for Philippine population of two species (Megaderma spasma and Hipposideros lekaguli) for the first time. Moreover, we present geographical variations in call frequencies for some species by comparing previously reported acoustic data elsewhere across the species’ range. This underscores the importance of establishing a readily accessible and comprehensive local reference library of echolocation calls which would serve as a valuable resource for examining taxonomic identities of echolocating bats, particularly those whose calls exhibit biogeographic variations.

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DNA Barcoding and Morphological Analyses Reveal a Cryptic Species of Miniopterus from India and Sri Lanka

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 7 publications

References 60 publications

Comparative virome analysis of individual shedding routes of Miniopterus fuliginosus bats inhabiting the Wavul Galge Cave, Sri Lanka

Comparative virome analysis of individual shedding routes of Miniopterus fuliginosus bats inhabiting the Wavul Galge Cave, Sri Lanka

Comparative virome analysis of individual shedding routes of Miniopterus phillipsi bats inhabiting the Wavul Galge cave, Sri Lanka

Echolocation call characterization of insectivorous bats from caves and karst areas in southern Luzon Island, Philippines

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