DNA barcoding

Ankola, Kunal; Mahadevegowda, LikhithGowda; Melichar, T.; Boregowda, Manjunatha H.

doi:10.1016/b978-0-12-820595-2.00018-7

Cited by 5 publications

(23 citation statements)

References 43 publications

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“…This is only practically feasible when the DNA barcodes have a low intraspecific and high interspecific variability, so the use of one barcode is not enough for all taxons. Hence, different barcodes are utilized for different groups of organisms" [8] (Table 1). The mitochondrial DNA is most favoured over nuclear DNA for barcode development due to the lack of introns and less recombination followed by a haploid of inheritance [9].…”

Section: Steps Involved In Dna Barcoding Procedures 1) Extraction And...mentioning

confidence: 99%

“…The mitochondrial DNA is most favoured over nuclear DNA for barcode development due to the lack of introns and less recombination followed by a haploid of inheritance [9]. Mitochondrial Cytochrome oxidase subunit 1 which is amplified to 648 bp is universally utilized as a standard barcode for animals followed by Cytb, 12S, or 16S [8]. Cytochrome oxidase subunit 1 is a maternally inherited mitochondrial enzyme complex that plays an important role in oxidative phosphorylation and is greatly conserved across living organisms [8].…”

Section: Steps Involved In Dna Barcoding Procedures 1) Extraction And...mentioning

confidence: 99%

“…Selection of primers is based on two criteria: -Versatility across a wide range of species and Affinity toward DNA with a balanced melting temperature. Due to this criterion, Cytochrome oxidase subunit 1 (COI) is considered as a universal primer for most animals and insects [8]. In some taxon, mini-barcoding is performed where this universal primer is not beneficial instead primers such as 16S rDNA are developed.…”

Section: ) Barcode and Polymerase Chain Reactionmentioning

confidence: 99%

“…Heat-stable DNA polymerases, the annealing temperature, and dNTP mixtures along with specific PCR conditions. Therefore, it is necessary to select more precise primers and PCR conditions for better amplification of barcoding locus and use of DNA signature to develop species-specific barcode [8].…”

Section: ) Barcode and Polymerase Chain Reactionmentioning

confidence: 99%

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Applications of DNA Barcoding in Fisheries: A Review

Shetty,

Shingadia

2023

UPJOZ

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The review article focuses on the widespread application of DNA barcoding in the fishery background. With the advancement in science and technology, there is a dire necessity for upgradation in approaches for taxonomy, wildlife conservation, and health management in the fishery industry. To reduce invasiveness, illegal fishing, and health complications in fish in both marine as well aquaculture ecosystems different required mitigative measures need to be enforced. DNA barcoding is one important tool in biological science. It involves the sequencing of a small DNA segment called a ‘barcode’ of 648 base pairs. In animals and protists, the specific barcode is the mitochondrial gene cytochrome oxidase Ⅰ (COⅠ or COX 1) and it has proven to be extremely effective in identifying metazoans such as birds, butterflies, fish, flies, and many other animal groups. The use of DNA Barcoding has been proven to be effective in taxonomical classification of every group of organisms. In fishery, the application of barcoding created an avenue in marine conservation and aquaculture caretaking.

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Section: Steps Involved In Dna Barcoding Procedures 1) Extraction And...mentioning

confidence: 99%

Section: Steps Involved In Dna Barcoding Procedures 1) Extraction And...mentioning

confidence: 99%

Section: ) Barcode and Polymerase Chain Reactionmentioning

confidence: 99%

Section: ) Barcode and Polymerase Chain Reactionmentioning

confidence: 99%

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Applications of DNA Barcoding in Fisheries: A Review

Shetty,

Shingadia

2023

UPJOZ

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“…This technique involves taking a body part of an organism, sequencing the DNA molecules contained in it through DNA amplification, and comparing it to databases with existing biological DNA sequences for identification. As of today, DNA barcoding is commonly used because it can be performed by anyone with the equipment and does not require any special knowledge (Ankola et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Advancing species identification: A non-invasive molecular approach through spider silk proteome analysis

Yamamoto,

Takasuka,

Mori

et al. 2024

Preprint

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Species identification is crucial in various scientific disciplines such as biology, ecology, medicine, and agriculture. While traditional methods rely on morphological characteristics, DNA barcoding has gained popularity due to its molecular biology approach. Nonetheless, DNA barcoding can be problematic for small animals such as insects, as it requires damaging their bodies for DNA extraction, impacting subsequent breeding and experiments. In this paper, we propose a non-invasive molecular method for species identification that examines the protein composition of animal produced biomaterials. We chose spider silk, with species-specific protein sequences, as our subject of analysis. First, we established a universal silk-dissolving method that applies to silks from various species. We constructed a bioinformatics pipeline employing metrics of significant difference through proteomic analysis to identify spider species by analyzing peptide sequences present in silk proteins. As a result, we achieved a species identification accuracy of 86% across 15 species. An appropriate reference dataset was successfully created, in addition, we also discovered some species are difficult to distinguish due to sequence similarities. This technology has been confirmed to be applicable to spider webs taken from the field. This non-invasive approach can complement DNA barcoding, especially in situations where it is infeasible, such as in studies involving spider-parasitoid wasps that eat spiders. Furthermore, it can be applied to other organisms that release biological substances, such as silkworm pupae, termite digestive enzymes, and tick saliva, aiding in species identification and pest control efforts.

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