DNA barcodes, ISSR, RAPD and SCAR markers as potential quality control tools for molecular authentication of black and white mulberry

El-Haggar, Miran A.; Mahgoub, Yasmin A.; Aly, Hesham M.; Ghazy, Nabila M.; El-Fiky, Fathy K.; El-Hawiet, Amr

doi:10.1016/j.foodcont.2023.109821

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…ISSR primers can be used to target microsatellites found throughout the plant genome. Therefore, the markers have been found to be more repeatable than others, such as RAPD [58]. Five primers amplified 70 loci across 11 cultivars.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Relationships among Indian Jujube (Ziziphus mauritiana Lamk.) Cultivars Using Morphometric Characteristics, matK Barcoding, and ISSR Markers

Abdel-Sattar,

Al-Obeed,

Rihan

et al. 2024

Diversity

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Indian jujube displays genetic diversity and does not prominently display minute morphometric variations, and this makes correct identification a difficult and long-term task. However, little work has been conducted to bring jujube cultivars into domestication. So, the present study aimed to evaluate eleven cultivars of Indian jujube in terms of the fruit’s morphometric characteristics, as well as molecular marker studies by plastidial megakaryocyte-associated tyrosine kinase (matK) barcoding and inter-simple sequence repeats (ISSR) markers for species differentiation, identification, and relationships among Indian jujube cultivars. The results of the morphometric characteristics showed that the mean geometric diameter, surface area, sphericity, sphericity ratio, shape index, fruit length, fruit diameter, fruit weight, and seed weight varied among cultivars. The results also showed that the color values of L*, a*, and b* for fruits differed in different cultivars. In addition, the results showed a discrepancy in the genetic diversity parameters related to the matK barcoding, ISSR markers, and relationships among Indian jujube cultivars. Substantially, hierarchical clustering by heatmap revealed that ‘Zytoni’ and ‘Um-Sulaem’ with spines seem to be mono-clades distinct from other cultivars, which related to variations in the expression levels of genes. Therefore, they should be relied upon together to distinguish and identify cultivars in order to maximize the effectiveness of local germplasm conservation and exploitation.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Relationships among Indian Jujube (Ziziphus mauritiana Lamk.) Cultivars Using Morphometric Characteristics, matK Barcoding, and ISSR Markers

Abdel-Sattar,

Al-Obeed,

Rihan

et al. 2024

Diversity

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“…Using the electrophoresis spectrum of the DL 2000 marker as a reference, the bands were manually reviewed according to the electrophoretic mobility of each band. The clear bands were recorded as “1”, while those with missing or weak bands were recorded as “0”, and the original binary data matrix of the 0/1 format was established using Excel 2019 software [ 27 ]. The “0/1 matrix” was imported into the POPGene 32 software to calculate the genetic diversity index of six populations, including the number of polymorphic loci, number of alleles ( N a ), effective number of alleles ( N e ), Nei’s genetic diversity ( H e ), Shannon diversity index ( I ), percentage of polymorphic loci ( PPL ), total genetic diversity ( H t ), genetic diversity among populations ( H s ), gene differentiation coefficient ( G st ), and gene flow ( N m ) [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

The Genetic Diversity and Genetic Structure of the Germplasm Resources of the Medicinal Orchid Plant Habenaria dentata

Yang,

Tang,

Zou

et al. 2023

Genes

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Habenaria dentata has medicinal and ornamental value, but the number of wild populations is decreasing dramatically. Thus, conducting research on its genetic diversity and structure is necessary to provide a basis for its conservation. This study aimed to explore the genetic diversity of the wild plant H. dentata and protect and optimize its wild resources. The genetic diversity of 133 samples from six wild populations of H. dentata was analyzed using Inter Simple Sequence Repeat molecular markers to provide a scientific basis for the screening of improved germplasm resources. The results showed that the average number of alleles was 1.765, the average number of effective alleles was 1.424, the average Nei’s gene diversity index was 0.252, the average Shannon diversity index was 0.381, and the average percentage of polymorphic loci was 76.499%. The variation within the populations was 77.34%, and the variation between the populations was 22.66%. The gene flow was 1.705, which was greater than 1. The results of the cluster analysis showed that the six populations were mainly divided into four clusters and were not classified according to their geographical location. There was no significant correlation between the geographical location and genetic distance between the populations (r = 0.557, p > 0.05). The genetic diversity of H. dentata is high. Among the six wild populations, the genetic diversity of the Mulun population was the highest and this population can be used as a key protection unit. The study on the genetic diversity of H. dentata can not only reveal the reasons for the decrease in the number of individuals in the population to a certain extent, and put forward the protection strategy, but also provide a scientific basis for the breeding of excellent seed resources.

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Genetic diversity analysis of Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu based on ISSR

Zheng,

Xu,

Yang

et al. 2024

Journal of Holistic Integrative Pharmacy

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DNA barcodes, ISSR, RAPD and SCAR markers as potential quality control tools for molecular authentication of black and white mulberry

Cited by 4 publications

References 42 publications

Genetic Diversity and Relationships among Indian Jujube (Ziziphus mauritiana Lamk.) Cultivars Using Morphometric Characteristics, matK Barcoding, and ISSR Markers

Genetic Diversity and Relationships among Indian Jujube (Ziziphus mauritiana Lamk.) Cultivars Using Morphometric Characteristics, matK Barcoding, and ISSR Markers

The Genetic Diversity and Genetic Structure of the Germplasm Resources of the Medicinal Orchid Plant Habenaria dentata

Genetic diversity analysis of Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu based on ISSR

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