DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

Ashfaq, Muhammad; Hebert, Paul D. N.

doi:10.1139/gen-2016-0024

Cited by 57 publications

(48 citation statements)

References 148 publications

(136 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA barcoding has aided our understanding of species community compositions, food webs and genetic variation within species (Baker et al 2016;Littlefair & Clare 2016;Roslin & Majaneva 2016) and is an important and useful asset in biosecurity (Ashfaq & Hebert 2016;DOI 10.1163/1876312X-00002172 Hodgetts et al 2016) and biomonitoring of freshwater ecosystems (Brodin et al 2013;Carew et al 2013). DNA barcodes can uncover cryptic species diversity (Macher et al 2016;Witt et al 2006;Yang et al 2012), and indicate species boundaries with additional morphological and ecological data.…”

Section: Introductionmentioning

confidence: 99%

DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera)

Stur

Ekrem

2018

Insect Syst. Evol.

View full text Add to dashboard Cite

For over a decade, DNA barcoding has proven an effective modern tool in taxonomy, evolutionary biology and biodiversity research. Many new species have been discovered and described with DNA barcodes as part of their diagnostic features. Using DNA barcodes, we uncovered a number of potential species within the Tanytarsus curticornis and Tanytarsus heusdensis species complexes (Diptera: Chironomidae) and detected morphological differences a posteriori that support the description of new species. Unusually large intraspecific divergence in COI p-distance (up to 10%) was observed for two species complexes. In total, eight species new to science are described and figured: T. adustus sp. n., T. heberti sp. n., T. madeiraensis sp. n., T. pseudoheusdensis sp. n., T. songi sp. n., T. thomasi sp. n., T. tongmuensis sp. n. and T. wangi sp. n.. Tanytarsus reei and T. tamaoctavus are redescribed, and T. tusimatneous is listed as a new junior synonym of T. tamaduodecimus. The diagnostic characters of the remaining species of the complexes are discussed. Keys to males and pupae are given.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera)

Stur

Ekrem

2018

Insect Syst. Evol.

View full text Add to dashboard Cite

show abstract

“…In fact, intraspecific divergence reached 16.7% in Coleophora trifolii , while specimens of Glyphodes onychinalis were assigned to six BINs. Prior studies have also reported cases of high intraspecific divergence and resultant BIN splits in Lepidoptera [57,58]. For example, 8% of the 1541 species of Noctuoidea analyzed from sites across Canada showed >2% intraspecific divergence and a similar incidence of BIN splits [13].…”

Section: Discussionmentioning

confidence: 99%

Mapping global biodiversity connections with DNA barcodes: Lepidoptera of Pakistan

Ashfaq

Akhtar

Rafi³

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Sequences from the DNA barcode region of the mitochondrial COI gene are an effective tool for specimen identification and for the discovery of new species. The Barcode of Life Data Systems (BOLD) (www.boldsystems.org) currently hosts 4.5 million records from animals which have been assigned to more than 490,000 different Barcode Index Numbers (BINs), which serve as a proxy for species. Because a fourth of these BINs derive from Lepidoptera, BOLD has a strong capability to both identify specimens in this order and to support studies of faunal overlap. DNA barcode sequences were obtained from 4503 moths from 329 sites across Pakistan, specimens that represented 981 BINs from 52 families. Among 379 species with a Linnaean name assignment, all were represented by a single BIN excepting five species that showed a BIN split. Less than half (44%) of the 981 BINs had counterparts in other countries; the remaining BINs were unique to Pakistan. Another 218 BINs of Lepidoptera from Pakistan were coupled with the 981 from this study before being compared with all 116,768 BINs for this order. As expected, faunal overlap was highest with India (21%), Sri Lanka (21%), United Arab Emirates (20%) and with other Asian nations (2.1%), but it was very low with other continents including Africa (0.6%), Europe (1.3%), Australia (0.6%), Oceania (1.0%), North America (0.1%), and South America (0.1%). This study indicates the way in which DNA barcoding facilitates measures of faunal overlap even when taxa have not been assigned to a Linnean species.

show abstract

“…It is now widely employed in practical contexts ranging from monitoring pests [14] and species at risk [15] to supporting the detection of invasive species [16] and suppressing food fraud [17] and deceptive herbal products [18]. Reflecting a major international effort to build a reference library of DNA barcodes for all known species, records for nearly 600,000 species are now available in the Barcode of Life Data Systems (BOLD) [19].…”

Section: Dna Barcodingmentioning

confidence: 99%

The School Malaise Trap Program: Coupling educational outreach with scientific discovery

et al. 2017

View full text Add to dashboard Cite

The School Malaise Trap Program (SMTP) provides a technologically sophisticated and scientifically relevant educational experience that exposes students to the diversity of life, enhancing their understanding of biodiversity while promoting environmental stewardship. Since 2013, the SMTP has allowed 15,000 students at 350 primary and secondary schools to explore insect diversity in Canadian schoolyards. Students at each school collected hundreds of insects for an analysis of DNA sequence variation that enabled their rapid identification to a species. Through this hands-on approach, they participated in a learning exercise that conveys a real sense of scientific discovery. As well, the students contributed valuable data to the largest biodiversity genomics initiative ever undertaken: the International Barcode of Life project. To date, the SMTP has sequenced over 80,000 insect specimens, which includes representatives of 7,990 different species, nearly a tenth of the Canadian fauna. Both surprisingly and importantly, the collections generated the first DNA barcode records for 1,288 Canadian species.

show abstract

DNA barcodes for bio-surveillance: regulated and economically important arthropod plant pests

Cited by 57 publications

References 148 publications

DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera)

DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera)

Mapping global biodiversity connections with DNA barcodes: Lepidoptera of Pakistan

The School Malaise Trap Program: Coupling educational outreach with scientific discovery

Contact Info

Product

Resources

About