DNA Barcode reveals mislabelling in the identification of marine fish swimming bladders for commercialization

Silva, Caio F. da; Daneluz, Cahique M.; Camacho-Oliveira, Raul B.; Prado, Fernanda Dotti do; Foresti, Fausto; Rodrigues, Carlos E.; Porto‐Foresti, Fábio

doi:10.1016/j.forsciint.2019.03.001

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…However, they are still sold on the market widely and even under the same trade name as sh maws from croakers. There is no doubt that equivocal species and different trade names lead to confusion in the sh maw market [14]. The identi cation results in this study increased our understanding of the species of sh maws on the market and provided a reference for the management of the sh maw market.…”

Section: Discussionmentioning

confidence: 73%

“…However, sh maws from croakers are replaced by maws from other sh species intentionally or unintentionally because of the lack of effective methods for species identi cation and classi cation. This has caused confusion in the sh maw market [10,14]. In the present study, various kinds of sh maw samples with different trade names, prices, morphotypes and sources were collected, and species identi cation was successfully performed using mtDNA 16S rRNA nucleotide sequences.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is di cult to accurately trace the sh species to which a maw belongs based on its shape, texture, size, thickness or other morphological characteristic [13]. This means that the same sh species may be sold under different names and at various prices due to the confusing relationships between species, names and prices [14]. Consumers also face a risk of paying very different prices for products of the same quality.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Commercial Fish Maws using mtDNA 16S rRNA Nucleotide Sequences

Zhang¹,

Deng²,

Li³

et al. 2020

Preprint

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Background: Fish maws (dried swim bladders of fish) have long been used as a medicinal material and valuable tonic food which are very popular in Southeast Asia. It is difficult to identify the original species of fish maws sold on the market due to a lack of taxonomic characteristics. The present study aims to investigate the origin of commercial fish maws using DNA sequencing based on a partial sequence of the mtDNA 16S rRNA gene.Methods: Mitochondrial genomic DNA was extracted from a total of 44 individual fish maw samples collected from different markets by a QIAamp® DNA Mini Kit. The fragments of the 16S rRNA gene were amplified by PCR and bidirectionally sequenced using an ABI 3730 genetic analyser. Sequence assembly was performed with DNASTAR SeqMan software, and the resulting sequence was compared with reference sequences in GenBank using BLAST. Interspecific divergence and intraspecific variation were calculated by the Kimura 2-parameter model. Sample sequences were clustered in MEGA 6 using a neighbour-joining tree.Results: Forty-two of 44 fish maw samples were sequenced successfully. Fourteen taxa matched known fish species in the DNA sequence database, and 10 of them were supported by high homogeneity (99-100%). According to the results, 88% of the total samples belonged to family Sciaenidae. The clustering and genetic distance results, including in-group divergence and out-group divergence, indicated conﬁdence in species identiﬁcations. Moreover, there was no strict correspondence between the commercial names provided by traders and the origin of the fish maws. This study also found a probable correlation between the molecular characteristics and morphological features of fish maws from croakers and non-croakers.Conclusion: In this study, DNA sequencing based on mtDNA 16S rRNA was carried out successfully to identify the origin of ~95% of the samples. This result indicated that 16S rRNA is a suitable barcode for identifying fish maws. The identification results allowed us to learn more about the fish species available in the fish maw market in Guangdong-Hongkong-Macao Great Bay. In addition, the association between trade category and species found in this study provides both consumers and merchants with an important reference for identifying the origin of fish maws.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Commercial Fish Maws using mtDNA 16S rRNA Nucleotide Sequences

Zhang¹,

Deng²,

Li³

et al. 2020

Preprint

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“…So far, a large number of studies have used mitochondrial sequences as DNA barcodes to identify fish species, such as cytochrome C oxidase subunit I ( COI ), cytochrome oxidase B ( Cytb ), and the control region (D-loop). COI was initially suggested to be an ideal standard DNA barcode for metazoans, and decades of research have proved its versatility in animals, including fish. ,− It is also widely applied to authenticate species in processed food. − Cytb gene has also been shown to be effective for fish species authentication. , Kappel et al demonstrated the feasibility of next-generation sequencing using two Cytb fragments to identify tuna components in the mixture tuna samples. Ma et al believed that Cytb gene was more suitable for the identification of Schizothorax spp.…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting Analysis of COI Sequences Distinguishes Pufferfish Species (Takifugu spp.) in China

Chen

Ding

Wang³

et al. 2021

J. Agric. Food Chem.

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Pufferfish is a traditional, delicious dish in Asia. However, eating wild or improperly processed pufferfish causes serious poisoning. This study aimed to exploit the high-resolution melting (HRM) method for authenticating four species of Takifugu pufferfish (Takifugu xanthopterus, T. fasciatus, T. flavidus, and T. rubripes). Candidate DNA barcodes, including the cytochrome c oxidase subunit I (COI), cytochrome oxidase b (Cytb), and the control region (D-loop), were analyzed, with COI selected as the optimal DNA barcode. An HRM method was developed to identify 57 commercial fish samples in China, including 33 commercial pufferfish products and 24 unlabeled fish products. The findings revealed that the pufferfish products were T. rubripes or T. fasciatus, and four T. xanthopterus samples were detected in unlabeled fish products. These results showed that DNA barcode coupled with HRM analysis was a rapid and efficient tool to identify pufferfish, which might aid in the prevention of consumer fraud or mislabeling of fish products.

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“…To align with international standards, the Taiwan Food and Drug Administration allows skipjack tuna to be used in 鮪 (for tuna)-labeled canned tuna products, even though it does not belong to the genus Thunnus, but other "pseudo-tunas" can no longer be used legally as a canned tuna ingredient. Seafood mislabeling is profuse worldwide [12][13][14][15][16][17][18][19][20][21]. Such mislabeling can be categorized into two types, i.e., deliberate or unintentional.…”

Section: Introductionmentioning

confidence: 99%

Product Authentication Using Two Mitochondrial Markers Reveals Inconsistent Labeling and Substitution of Canned Tuna Products in the Taiwanese Market

Chang

Kao

Huang

2021

Foods

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Fish of the tribe Thunnini represent a significant proportion of the stock caught by the fishing industry, with many of these fishes being collectively called tuna. However, only certain species can be used legally as an ingredient in canned tuna products, depending on regional food regulations. In Taiwan, only Thunnus species or Katsuwonus pelamis can be used as canned tuna. Here, we authenticated 90 canned tuna products, including 25 cat food samples, by sequencing two mitochondrial regions, 16S rRNA (16S) and the control region (CR). BLAST analysis revealed that Sarda orientalis, Euthynnus affinis, Auxis rochei, and Auxis thazard are all used as substitutes for legitimate tuna products. We found that 63.33% of investigated samples are true canned tuna, i.e., contain Thunnus species or skipjack tuna. We advocate that the Taiwanese government publishes an official standardized list of fishes, especially so that scientific, Chinese and vernacular names can be assigned unambiguously based on a “one species-one name policy”, thereby clarifying which species can be used in seafood products such as tuna. Furthermore, we feel that the large-scale and long-term monitoring of canned tuna products is warranted to fully assess the extent of tuna product adulteration in Taiwan.

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DNA Barcode reveals mislabelling in the identification of marine fish swimming bladders for commercialization

Cited by 9 publications

References 13 publications

Molecular Identification of Commercial Fish Maws using mtDNA 16S rRNA Nucleotide Sequences

Molecular Identification of Commercial Fish Maws using mtDNA 16S rRNA Nucleotide Sequences

High-Resolution Melting Analysis of COI Sequences Distinguishes Pufferfish Species (Takifugu spp.) in China

Product Authentication Using Two Mitochondrial Markers Reveals Inconsistent Labeling and Substitution of Canned Tuna Products in the Taiwanese Market

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