DNA Barcode Goes Two-Dimensions: DNA QR Code Web Server

Liu, Chang; Shi, Linchun; Xu, Xiaolan; Li, Huan; Xing, Hang; Liang, Dong; Jiang, Kun; Pang, Xiaohui; Song, Jingyuan; Chen, Shilin

doi:10.1371/journal.pone.0035146

Cited by 39 publications

(45 citation statements)

References 25 publications

(26 reference statements)

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“…The team acknowledged the practicality of this application, given the recent proliferation of QR coding on food products, although they did not actually implement their technology with end-users (Vasquez-Briseno, Nieto-Hipolito & Jimenez-Garcia, 2010). Finally, a group of researchers from China have reported success with using QR codes to represent DNA sequences since the QR stores a larger amount of information than the standard barcode and is amenable to information storage, recognition and retrieval (Liu, et al, 2012). Apart from these studies, no additional evidence of QR code use in health care was located.…”

Section: Qr Background and Applicationmentioning

confidence: 99%

Use of Quick Response Coding to Create Interactive Patient and Provider Resources

Bellot¹,

Shaffer²,

Wang³

2015

J Nurs Educ

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Background: Since their creation more than 20 years ago, the proliferation of Quick Response (QR) codes has expanded tremendously. Little was found in the literature to support the innovative use of QR coding in the classroom or in health care provision. Thus, the authors created a doctoral-level practicum experience using QR coding to create interactive, individualized patient or provider resource guides. Method: Short, descriptive surveys were used before and after implementation of the practicum experience to determine students’ comfort level using QR technology, their knowledge base, ease of use, and overall satisfaction with the practicum. Results: Students reported high levels of satisfaction with this exercise, and all agreed that use of QR coding could have important implications in the clinical environment. Conclusion: This practicum experience was a creative, practical, and valuable example of integrating emerging technology into individualized patient care. [ J Nurs Educ. 2015;54(4):224–227.]

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Section: Qr Background and Applicationmentioning

confidence: 99%

Use of Quick Response Coding to Create Interactive Patient and Provider Resources

Bellot¹,

Shaffer²,

Wang³

2015

J Nurs Educ

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“…Mobile terminals (such as Android and iPhone devices) can be used as QR code scanners to read the information. Finally, the information was made available online (http://www.tcmbarcode.cn) to identify and analyze ITS2 sequences [27,31].…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…The two-dimensional DNA barcode is the most appropriate tool for DNA sequences, with the largest coding capacity and relatively high compression ratio [27]. In this study, ITS2 sequences of Shi-Liang tea species and closely related species were converted into colored barcode images based on our software code (Figure 3) [31,33].…”

Section: Two-dimensional Dna Barcodes To Monitor Market Circulation Amentioning

confidence: 99%

“…Xin et al [33] combined QR codes and DNA barcodes to construct a two-dimensional DNA barcode system to regulate the circulation of TCM. Liu et al [27] compared different types of one-dimensional and two-dimensional barcodes for five DNA barcode markers, namely, ITS2, rbcL, matK, psbA-trnH, and CO1, and developed a web server for further use of QR code-based DNA barcodes. In this study, we obtained a two-dimensional DNA barcode for Shi-Liang tea, which can be used commercially to monitor the circulation of She medicine.…”

Section: Application and Significance Of Two-dimensional Dna Barcodesmentioning

confidence: 99%

“…This barcode has the largest coding capacity and relatively high compression ratio. The two-dimensional DNA is also similar to the barcode scanning used in supermarkets to distinguish different kinds of goods [27]. In this study, DNA barcoding technology was used to identify Shi-Liang tea (C. salicifolius and C. zhejiangensis) and five other species in the family Calycanthaceae using ITS2 to ensure the accuracy and safety of Calycanthaceae in traditional folk medicine practice.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Zhou

et al. 2017

Applied Sciences

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Traditional She medicine is part of China's cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ) tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine.

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