DNA Barcode as an effective tool in the identification of billfishes (Scombroidei, Teleostei) from exported specimens

Rodrigues, Carlos E.; Carvalho, Daniel Eduardo Visciano de; Garcia, Yasmin Sakimoto; Hashimoto, Diogo Teruo; Foresti, Fausto; Porto‐Foresti, Fábio

doi:10.1016/j.fsiae.2021.100028

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Extraction Pcr and Dna Sequencing1

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2024

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“…The thermocycling conditions consisted of initial denaturation at 95℃ (2 min) followed by denaturation at 94℃ (30 cycles, 45 s), annealing at 49.7 -60℃ (45 s); elongation at 72℃ (1 min), and final extension at 72℃ (10 min) before termination of the reaction at 4℃ [9,15]. The FishF1 primer region is typically short enough to be easily amplified using this PCR method, and its sequencing is technically feasible and facilitates efficient DNA barcoding workflow [25].…”

Section: Extraction Pcr and Dna Sequencingmentioning

confidence: 99%