DNA banks and their role in facilitating the application of genomics to plant germplasm

Rice, Nicole F; Cordeiro, Giovanni M; Shepherd, Mervyn; Bundock, Peter C; Bradbury, Louis Mt; Pacey-Miller, Toni; Furtado, Agnelo; Henry, Robert J.

doi:10.1079/pgr2006113

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…Domesticated or economically important species from all countries are also being collected and stored. The international networking of DNA banks will be a key step in linking genomics tools to global plant diversity (Rice et al 2006 ).…”

Section: Conservation Of Dnamentioning

confidence: 99%

Gene Banking for Ex Situ Conservation of Plant Genetic Resources

Rajasekharan

2015

Plant Biology and Biotechnology

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Seed storage is one of the most widespread and valuable ex situ approaches to conservation. Extensive expertise has been developed in this fi eld by agencies and institutions involved with plant genetic resources over the past 30 years. Seed banking has considerable advantages over other methods of ex situ conservation such as ease of storage, economy of space, relatively low labour demands and, consequently, the capacity to maintain large samples at an economically viable cost. Depending on the species, seeds are dried to suitably low moisture content according to an appropriate protocol. Typically this will be less than 5 %. The seeds then are stored at −18 °C or below. Because seed DNA degrades with time, the seeds need to be periodically replanted and fresh seeds collected for another round of long-term storage. There are about six million accessions, or samples of a particular population, stored as seeds in about 1,300 gene banks throughout the world as of 2006. The procedure for seed storage along with the latest development on gene banking is discussed in this chapter.

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Section: Conservation Of Dnamentioning

confidence: 99%

Gene Banking for Ex Situ Conservation of Plant Genetic Resources

Rajasekharan

2015

Plant Biology and Biotechnology

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“…The establishment of DNA banks facilitates this screening of DNA from large numbers of plant accessions (Rice et al 2006). Markers at the DNA level represent the ideal tool since they characterize individuals directly at the level of genotype, without any concern on the effect of environment, plant tissue and developmental stage and, furthermore, their number is virtually infinite (Mondini et al 2009).…”

Section: Molecular Assessmentmentioning

confidence: 99%

Genetic Diversity Assessment in European Cynara Collections

Pagnotta

Noorani²

2013

Genomics of Plant Genetic Resources

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“…Seeds, buds, meristems and cells have been successfully cryopreserved in a number of forest tree species (Stanwood, 1985;Ahuja, 1986Ahuja, , 1989Ahuja, , 1999Bonner, 1990;Ryynänen, 1996). In addition, conservation of DNA at 2 20 to 2 808C in genebanks offers prospects for the application of genomics to germplasm conservation (Adams, 1997;Rice et al, 2006). Cryopreservation of germplasm has not been researched in the redwoods so far, but this avenue offers new options for storage of germplasm of the redwoods for future exploitation.…”

Section: Storage Of Germplasm Under Sub-zero and Ultra-low Temperaturesmentioning

confidence: 99%

Strategies for conservation of germplasm in endemic redwoods in the face of climate change: a review

Ahuja

2011

Plant Genet. Res.

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This study reviews the various conservation strategies applied to the four redwood species, namely coast redwood (Sequoia sempervirens), Sierra redwood or giant sequoia (Sequoiadendron giganteum), dawn redwood (Metasequoia glyptostroboides) and South American redwood or alerce (Fitzroya cupressoides), which are endemic in the USA, China and South America, respectively. All four redwood genera belong to the family Cupressaceae; they are monospecific, share a number of common phenotypic traits, including red wood, and are threatened in their native ranges due to human activity and a changing climate. Therefore, the management objective should be to conserve representative populations of the native species with as much genetic diversity as possible for their future survival. Those representative populations exhibiting relatively high levels of genetic diversity should be selected for germplasm preservation and monitored during the conservation phase by using molecular markers. In situ and ex situ strategies for the preservation of germplasm of the redwoods are discussed in this study. A holistic in situ gene conservation strategy calls for the regeneration of a large number of diverse redwood genotypes that exhibit adequate levels of neutral and adaptive genetic variability, by generative and vegetative methods for their preservation and maintenance in their endemic locations. At the same time, it would be desirable to conserve the redwoods in new ex situ reserves, away from their endemic locations with similar as well as different environmental conditions for testing their growth and survival capacities. In addition, other ex situ strategies involving biotechnological approaches for preservation of seeds, tissues, pollen and DNA in genebanks should also be fully exploited in the face of global climate change.

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DNA banks and their role in facilitating the application of genomics to plant germplasm

Cited by 11 publications

References 36 publications

Gene Banking for Ex Situ Conservation of Plant Genetic Resources

Gene Banking for Ex Situ Conservation of Plant Genetic Resources

Genetic Diversity Assessment in European Cynara Collections

Strategies for conservation of germplasm in endemic redwoods in the face of climate change: a review

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