DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis

Bayraç, Ceren; Eyidoğan, Füsun; Öktem, Hüseyin Avni

doi:10.1016/j.bios.2017.06.029

Cited by 102 publications

(50 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3C). In addition, aptamer-based colorimetric strategies for the detection of pathogenic cells have also been developed (Wu et al, 2012;Bayraç et al, 2017;Bruno, 2014;Yuan et al, 2014aYuan et al, , 2014bHu et al, 2017;Lavu et al, 2016). Consequently, the presence of free protamine is detected by a potential change of a polycation-sensitive membrane electrode.…”

Section: Aptamer-based Detection Of Pathogenic Whole Cellsmentioning

confidence: 99%

Nucleic acid aptamer-based methods for diagnosis of infections

Park

2018

Biosensors and Bioelectronics

137

View full text Add to dashboard Cite

Infectious diseases are a serious global problem, which not only take an enormous human toll but also incur tremendous economic losses. In combating infectious diseases, rapid and accurate diagnostic tests are required for pathogen identification at the point of care (POC). In this review, investigations of diagnostic strategies for infectious diseases that are based on aptamers, especially nucleic acid aptamers, oligonucleotides that have high affinities and specificities toward their targets, are described. Owing to their unique features including low cost of production, easy chemical modification, high chemical stability, reproducibility, and low levels of immunogenicity and toxicity, aptamers have been widely utilized as bio-recognition elements (bio-receptors) for the development of infection diagnostic systems. We discuss nucleic acid aptamer-based methods that have been developed for diagnosis of infections using a format that organizes discussion according to the target pathogenic analytes including toxins or proteins, whole cells and nucleic acids. Also included is, a summary of recent advances made in the sensitive detection of pathogenic bacteria utilizing the isothermal nucleic acid amplification method. Lastly, a nucleic acid aptamer-based POC system is described and future directions of studies in this area are discussed.

show abstract

Section: Aptamer-based Detection Of Pathogenic Whole Cellsmentioning

confidence: 99%

Nucleic acid aptamer-based methods for diagnosis of infections

Park

2018

Biosensors and Bioelectronics

137

View full text Add to dashboard Cite

show abstract

“…Therefore, it is considerable to develop very sensitive, selective, robustic and economical strategies which are suitable for onsite detection and quantification of pathogenic bacteria and may very beneficial to prevent and control several infectious and foodborne disease (Majdinasab et al 2018). Major efforts made in the direction of aptamers, high-end biosensors and aptaassays development are generally based on the optical methods include colorimetry (Bayraç et al 2017;Wu et al 2017), surface-enhanced Raman scattering (SERS) (Duan et al 2016a, c;Zhang et al 2015) chemiluminescence (Hao et al 2017a, b) fluorescence (Duan et al 2012) and electrochemical methods (Bayraç et al 2017;Luo et al 2012). Although colorimetry is the most commonly employed method, it offers low sensitivity; thus, fluorescence based detection methods are quite popular due to their higher sensitivity limit as compared to other assays.…”

Section: Aptamers In Bacteria Detectionmentioning

confidence: 99%

“…Similarly, Chen et al (2007) have also used whole cell SELEX strategy for the development of aptamer against Mycobacterium tuberculosis (H37Rv strain) (Chen et al 2007). DNA aptamers against a large number of pathogenic bacteria which can specifically bind to the various components of the bacterial cell have been developed and reported and potentially used as prospective therapeutic agents and diagnostic tools (Bayraç et al 2017;Brosel-Oliu et al 2018;Bruno and Sivils 2017;Dinshaw et al 2017;Duan et al 2016b, c;Hao et al 2017a;Sidhu et al 2016;Wu et al 2017).…”

Section: Aptamers In Bacteria Detectionmentioning

confidence: 99%

Recent Updates for Isolation of Aptamers for Various Biothreat Agents Using Different Strategies and Their Role in Detection Applications

Kumar

Sharma

2019

Aptamers

View full text Add to dashboard Cite

Aptamers, including naturally existing riboswitches, are single-stranded nucleic acids (DNA or RNA) and peptides molecules, which show the high affinity, selectivity, specificity, and versatility. Aptamers are selected by using the systematic evolution of ligands by exponential enrichment (SELEX) method against a particular target molecule. Aptamers can target the variety of molecules like proteins, carbohydrates, bacteria, viruses, toxins, and cancerous cells and can discriminate between closely related ligands. Aptamers used various strategies to sense the target binding like electrochemical, optical, mass-sensitive analytical technique etc. Thus, aptamers magnetizes the immense interest in the field of pathogen diagnostic and detection. In this chapter, we describe how the different strategies have updated for the isolation and selection of more efficient, novel and specific aptamers. In the last, we also provide insights about the potential aptamers used for the detection several biothreat agents like bacteria and parasites, and viruses.

show abstract

“…DNA molecules were single stranded DNA (ssDNA) used in previous study (Bayrac et al, 2017). They were supplied by Integrated DNA Technologies, Inc. (Iowa, USA) and their sequences were given at Table 1.…”

Section: Louismentioning

confidence: 99%

Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması

Bayraç

Sarikaya³

et al. 2019

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

Self Cite

View full text Add to dashboard Cite

Based on their unique properties various iron magnetic nanoparticles have proved to be excellent nanomaterials for applications in separation and concentration process. Immobilization of deoxyribonucleic acid (DNA) molecules on the magnetic beads are acutely important and have potential uses in many techniques such as DNA extraction, concentration, biosensors, microarrays and next generation sequencing. In this study the adsorption of single stranded DNA (ssDNA) via poly-l-lysine coated iron oxide magnetic beads was performed under varying conditions of poly-l-lysine amount, initial DNA concentration, ionic strength, bonding type and length of DNA. The adsorption process was examined via Langmuir and Freundlich isotherm models. The ionic interaction between negatively charged DNA and positively charged surface of magnetic beads showed multilayer adsorption with Freundlich adsorption isotherm, covalent bonding between modified DNA and surface by crosslinking provided higher adsorption efficiency with Langmuir adsorption isotherm. Both adsorption methods provided magnetic beads with favorable adsorption of ssDNA.

show abstract

DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis

Cited by 102 publications

References 45 publications

Nucleic acid aptamer-based methods for diagnosis of infections

Nucleic acid aptamer-based methods for diagnosis of infections

Recent Updates for Isolation of Aptamers for Various Biothreat Agents Using Different Strategies and Their Role in Detection Applications

Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması

Contact Info

Product

Resources

About