DNA Analysis Detects Different Mislabeling Trend by Country in European Cod Fillets

Feldmann, Frederik; Ardura, Alba; Blanco-Fernandez, Carmen; García‐Vázquez, Eva

doi:10.3390/foods10071515

Cited by 12 publications

(11 citation statements)

References 47 publications

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“…Processed food products mixed with Atlantic cod and haddock have been reported in previous studies. For Atlantic cod, haddock was the most popular substitute species in France, Sweden, and Denmark [8]. Similar cases were found in Italy and Spain [7,28].…”

Section: Monitoringsupporting

confidence: 63%

“…Mislabeled products associated with Atlantic cod and haddock were expected to be reported more because those two fishes were repeatedly caught in a mixed fishery [29]. Haddock was also listed as the frequently replaced cod species in the IUCN database [7,8]. Most cod products were labeled only "cod" without representing a specific species.…”

Section: Monitoringmentioning

confidence: 99%

“…For instance, it is reported that the price of Atlantic cod is comparatively higher than that of the other Gadidae species (i.e., haddock and Alaska pollock) [5]. Likewise, several studies have confirmed cases of Atlantic cod being substituted with Pacific cod, Alaska pollock, or haddock [6][7][8][9]. Additionally, the error rate of cod labeling ranged from 0% to 60% in nine North Atlantic countries, and 7.4% and 28.4% labeling errors were found from 226 cod products in the UK and Ireland, respectively [10].…”

Section: Introductionmentioning

confidence: 99%

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A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

Lee

Kim

2021

Foods

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With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed fish products after processing because of their similar morphological characteristics. Substitution and mislabeling of codfish among different species are also happening deliberately or unintentionally. Thus, it is necessary to distinguish cod species to prevent fish adulteration and food fraud. In this study, we developed a multiplex PCR for simultaneously identifying five cod species within Gadidae using capillary electrophoresis. Then, their species-specific primer sets were designed by targeting the mitochondrial cytochrome b gene. Subsequently, the amplicon sizes obtained were 237 bp, 204 bp, 164 bp, 138 bp, and 98 bp for Atlantic cod, Pacific cod, blue whiting, haddock, and Alaska pollock, respectively. The specificity of each primer was further tested using 19 fish species, and no cross-reactivity was observed. The limit of detection of this multiplex PCR assay was 1 pg. The developed multiplex PCR assay can be applied to 40 commercial food products successfully. This detection method will be efficient for managing seafood authentication by simultaneously analyzing multiple cod species.

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Section: Monitoringsupporting

confidence: 63%

Section: Monitoringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

Lee

Kim

2021

Foods

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“…Fish mislabeling is a global issue, affecting both food security and biodiversity conservation (Staffen et al 2017). This practice, which has been identified in several countries (Feldmann et al 2021), involves the intentional or unintentional misidentification of fish sold in markets or restaurants (Galimberti et al 2013). Intentional mislabeling often involves the fraudulent sale of fish branded as having a higher economic or cultural value (Donlan and Luque 2018).…”

Section: Fish Mislabeling: a Global Issuementioning

confidence: 99%

Unveiling the Threats Beneath: Fish Mislabeling in the Brazilian Amazon Coast and its Impacts on the Critically Endangered Daggernose Shark

et al. 2023

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Fish mislabeling is a global issue with far‐reaching consequences for both food security and biodiversity conservation. This practice involves the intentional or unintentional misidentification of fish species sold in markets and restaurants, often driven by economic gain or cultural preferences. The mislabeling of threatened fish species further exacerbates the problem. This manuscript focuses on an emerging mislabeling practice in the Brazilian Amazon coast, which impacts a critically endangered elasmobranch species, the Daggernose Shark Isogomphodon oxyrhynchus. More specifically, the mislabeling of the Atlantic Tripletail Lobotes surinamensis as the Acoupa Weakfish Cynoscion acoupa has led to intensified fishing activities and incidental capture of the Daggernose Shark. Traditional enforcement approaches have shown limited effectiveness, and alternative conservation interventions, including participatory management and education programs for artisanal fishers, are needed to address this complex issue.

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“…Atlantic cod ( Gadus morhua ) is characterized by an excellent source of quality proteins, polyunsaturated fat and essential micronutrients, 1 and represents one of the most popular species in the Gadidae family. However, due to declining stocks in most source areas of the world, indistinguishable features for processed products, and poor transparency in the global supply chain, species adulteration frequently occurs with Atlantic cod 2‐4 . Moreover, a great variety of species have been identified in Atlantic cod products, including Pacific cod ( G. macrocephalus ), 2 Alaska pollock ( Theragra chalcogramma ), 5 saithe ( Pollachius virens ), 6 European hake ( Merluccius merluccius ), 7 Greenland halibut ( Reinhardtius hippoglossoides ), 3 anglerfish ( Lophius litulon ), 8 oilfish ( Ruvettus pretiosus , Lepidocybium flavobrunneum ) 9 and even pufferfish ( Lagochephalus spp.)…”

Section: Introductionmentioning

confidence: 99%

Closed‐tube visual detection of Atlantic cod (Gadus morhua) using self‐quenched primer coupled with a designed loop‐mediated isothermal amplification vessel

et al. 2023

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BACKGROUND Species adulteration has been widely revealed around the world, and the possible reasons include declining stocks in most source areas of the world, poor transparency in the global supply chain, and difficulty in distinguishing the features of processed products. The present work selected Atlantic cod (Gadus morhua) as a case study, and developed a novel loop‐mediated isothermal amplification (LAMP) assay for Atlantic cod authentication, where a self‐quenched primer and a newly designed reaction vessel were used to realize the endpoint visual detection of the target‐specific products. RESULTS A novel LAMP primer set was designed for Atlantic cod, and the inner primer BIP was selected to label the self‐quenched fluorogenic element. The fluorophore's dequenching only occurred along with LAMP elongation for the target species. No fluorescence could be observed with both single‐stranded DNA and partially complementary dsDNA of the non‐target species. With the novel reaction vessel, both amplification as well as detection were operated in an enclosed device, and visual differentiation of Atlantic cod, negative control, and false positive generated from primer dimers was achieved. The novel assay has proved its specificity and applicability, and could detect as little as 1 pg of Atlantic cod DNA. Moreover, Atlantic cod adulteration as low as 10% could be detected in haddock (Melanogrammus aeglefinus), and no cross‐reactivity was observed. CONCLUSION The established assay could be a useful tool to detect mislabeling incidents involving Atlantic cod considering the advantages of speed, simplicity and accuracy. © 2023 Society of Chemical Industry.

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DNA Analysis Detects Different Mislabeling Trend by Country in European Cod Fillets

Cited by 12 publications

References 47 publications

A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

Unveiling the Threats Beneath: Fish Mislabeling in the Brazilian Amazon Coast and its Impacts on the Critically Endangered Daggernose Shark

Closed‐tube visual detection of Atlantic cod (Gadus morhua) using self‐quenched primer coupled with a designed loop‐mediated isothermal amplification vessel

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