DNA analysis by MALDI-TOF mass spectrometry

Gut, Ivo

doi:10.1002/humu.20023

Cited by 108 publications

(52 citation statements)

References 35 publications

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“…The potential of MALDI-TOF using metabolomic approaches is demonstrated by the successful profiling of low-molecular mass volatile compounds in the headspace (the air or empty space left above the contents in a sealed container) of C. albicans urine suspensions by another MS method, proton-transfer reaction MS [188]. MALDI-TOF instruments can record in excess of 10 spectra per second; since a mass spectrum provides many detection channels, a MALDI-TOF analysis is suitable for highthroughput, multichannel detection and quantitative analysis of DNA [189] and RNA [190]. Consequently, this method has become a powerful platform for the future science group study of nucleic acid sequence [191] and expression level changes [168] and holds tremendous potential, as yet untapped, for specific detection of fungal pathogens.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Biomarkers for Invasive Aspergillosis: The Challenges Continue

et al. 2014

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The incidence of invasive aspergillosis (IA), an opportunistic infection in immuno compromised individuals, is rising, but its early diagnosis remains challenging and treatment options are limited. Hence there is an urgent need to improve existing diagnostic procedures as well as develop novel approaches. The clinical usefulness of galactomannan and βdglucan, widely used assays detecting cellwall antigens of Aspergillus, is unclear and depends on clinicians' awareness of their practical limitations. This leaves room for new methods that utilize genomic, proteomic and metabolomics approaches as well as novel detection procedures, for example point ofcare lateralflow devices. Each of these strategies has its own limitations and it is likely that a combination of methods will be required to achieve optimal performance for the diagnosis of IA and subsequent appropriate patient management.

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Section: Mass Spectrometrymentioning

confidence: 99%

Biomarkers for Invasive Aspergillosis: The Challenges Continue

et al. 2014

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“…Analysis is carried out by MALDI-TOF mass spectrometry. Differences between the unknown sample and a reference sequence are determined by changes in the results pattern [21,22].…”

Section: Dna Sequencing By Maldi Tof Mass Spectrometrymentioning

confidence: 99%

Genetic Engineering and Cloning: Focus on Animal Biotechnology

Giassetti¹,

Maria²,

Assumpção³

et al. 2013

Genetic Engineering

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“…The negative ion mode has been less frequently employed, with the exception of its application toward specific compound classes, such as DNA [8] , sugars [1], and lipids [9].…”

Section: Introductionmentioning

confidence: 99%

Negative Ion MALDI Mass Spectrometry of Polyoxometalates (POMs): Mechanism of Singly Charged Anion Formation and Chemical Properties Evaluation

Boulicault

Alvès

Cole

2016

J. Am. Soc. Mass Spectrom.

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Abstract. MALDI-MS has been developed for the negative ion mode analysis of polyoxometalates (POMs). Matrix optimization was performed using a variety of matrix compounds. A first group of matrixes offers MALDI mass spectra containing abundant intact singly charged anionic adduct ions, as well as abundant in-source fragmentations at elevated laser powers. A relative ranking of the ability to induce POM fragmentation is found to be: DAN > CHCA > CNA > DIT> HABA > DCTB > IAA. Matrixes of a second group provide poorer quality MALDI mass spectra without observable fragments. Sample preparation, including the testing of salt additives, was performed to optimize signals for a model POM, POMc12, the core structure of which bears four negative charges. The matrix 9-cyanoanthracene (CNA) provided the best signals corresponding to singly charged intact POMc12 anions. Decompositions of these intact anionic species were examined in detail, and it was concluded that hydrogen radical-induced mechanisms were not prevalent, but rather that the observed prompt fragments originate from transferred energy derived from initial electronic excitation of the CNA matrix. Moreover, in obtained MALDI mass spectra, clear evidence of electron transfer to analyte POM species was found: a manifestation of the POMs ability to readily capture electrons. The affinity of polyanionic POMc12 toward a variety of cations was evaluated and the following affinity ranking was established: Fe 3+ > Al 3+ > Li + > Ga 3+ > Co 2+ > Cr 3+ > Cu 2+ > [Mn 2+ , Mg 2+ ] > [Na + , K + ]. Thus, from the available cationic species, specific adducts are preferentially formed, and evidence is given that these higher affinity POM complexes are formed in the gas phase during the early stages of plume expansion.

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DNA analysis by MALDI-TOF mass spectrometry

Cited by 108 publications

References 35 publications

Biomarkers for Invasive Aspergillosis: The Challenges Continue

Biomarkers for Invasive Aspergillosis: The Challenges Continue

Genetic Engineering and Cloning: Focus on Animal Biotechnology

Negative Ion MALDI Mass Spectrometry of Polyoxometalates (POMs): Mechanism of Singly Charged Anion Formation and Chemical Properties Evaluation

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